CAJ | 학술논문

利用电喷雾质谱(ESI-MS)法建立龙胆质谱指纹图谱,研究其中代表性裂环烯醚萜苷类成分的ESI-MS规律.采用甲醇超声法提取龙胆中裂环烯醚萜苷类成分,在正离子方式检测模式下直接进样,应用一级全扫描质谱建立其特征图谱.运用化学模式识别方法对图谱数据进行分析,发现秋季龙胆样品的聚集程度优于春季龙胆样品,根据春季、秋季药材样品的差异,可以对采收期进行区分;裂环烯醚萜苷类成分在二级质谱中易发生脱水、乙酰基、葡萄糖基等.电喷雾质谱法的精密度、稳定性、重现性均符合要求,且具有特征性强、分析速度快、样品用量少等优点.
이용전분무질보(ESI-MS)법건립룡담질보지문도보,연구기중대표성렬배희미첩감류성분적ESI-MS규률.채용갑순초성법제취룡담중렬배희미첩감류성분,재정리자방식검측모식하직접진양,응용일급전소묘질보건립기특정도보.운용화학모식식별방법대도보수거진행분석,발현추계룡담양품적취집정도우우춘계룡담양품,근거춘계、추계약재양품적차이,가이대채수기진행구분;렬배희미첩감류성분재이급질보중역발생탈수、을선기、포도당기등.전분무질보법적정밀도、은정성、중현성균부합요구,차구유특정성강、분석속도쾌、양품용량소등우점.
The fingerprint of Gentiana scabra Bge. was established by electrospray ionization mass spectrometry (ESI-MS). Main secoiridoid glycosides of Gentiana scabra Bge. and their fragmentment pathway were analyzed by ESI-MS. Secoiridoid glycosides were extracted form Gentiana scabra Bge. by methanol supersonics,and extracts were analyzed directly in positive ion mode by ESI-MS. The results of ESI-MS fingerprint were processed by chemical pattern recognition method. The samples form autumn are in good quality. The result also can differentiate samples from different seasons. Secoiridoid glycosides are fragmented through losing H_2O, acetyl and glucosyl in ESI-MS~2. Electrospray ionization mass spectrometry method is good precision, stability and reproducibility. This method is simple, rapid, and can be used for identification and study of characteristic fingerprint.