东北林业大学学报
東北林業大學學報
동북임업대학학보
JOURNAL OF NORTHEAST FORESTRY UNIVERSITY
2010年
2期
79-82
,共4页
苏凤艳%魏吉祥%王卓聪%王春雨%王全凯
囌鳳豔%魏吉祥%王卓聰%王春雨%王全凱
소봉염%위길상%왕탁총%왕춘우%왕전개
水貂%犬瘟热%分离鉴定
水貂%犬瘟熱%分離鑒定
수초%견온열%분리감정
Mink%Canine distemper virus%Isolation and identification
从疑似犬瘟热病死水貂的肝脏中分离出1株病毒,并进行了系统的鉴定.结果表明:该毒株接种于Vero传代细胞后,出现了典型而有规律的病变;其病毒理化特性与犬瘟热病毒相同;致细胞病变作用可被兔抗CDV阳性血清阻断;间接免疫荧光试验表明接种病毒的BHK-21细胞出现特异性的亮绿色荧光,而正常细胞则未见绿色荧光;对提取接种病料后的Vero细胞培养物中的总RNA进行RT-PCR扩增,得到了324bp和1053bp的目的片段,证明该毒株为CDV.
從疑似犬瘟熱病死水貂的肝髒中分離齣1株病毒,併進行瞭繫統的鑒定.結果錶明:該毒株接種于Vero傳代細胞後,齣現瞭典型而有規律的病變;其病毒理化特性與犬瘟熱病毒相同;緻細胞病變作用可被兔抗CDV暘性血清阻斷;間接免疫熒光試驗錶明接種病毒的BHK-21細胞齣現特異性的亮綠色熒光,而正常細胞則未見綠色熒光;對提取接種病料後的Vero細胞培養物中的總RNA進行RT-PCR擴增,得到瞭324bp和1053bp的目的片段,證明該毒株為CDV.
종의사견온열병사수초적간장중분리출1주병독,병진행료계통적감정.결과표명:해독주접충우Vero전대세포후,출현료전형이유규률적병변;기병독이화특성여견온열병독상동;치세포병변작용가피토항CDV양성혈청조단;간접면역형광시험표명접충병독적BHK-21세포출현특이성적량록색형광,이정상세포칙미견록색형광;대제취접충병료후적Vero세포배양물중적총RNA진행RT-PCR확증,득도료324bp화1053bp적목적편단,증명해독주위CDV.
A strain of canine distemper virus (CDV) was isolated and identified systemically from the liver of a dead mink suspected of having canine distemper. Results indicated that typical and disciplinary pathological changes appeared on Vero cells after being inoculated with the treated pathological material, and the physical and chemical characteristics of the isolated virus were similar to those of CDV. The cytopathogenic effect was interdicted by rabbit anti-CDV positive serum. Indirect immunofluorescent assay showed that idiosyncratic bright green fluorescence appeared on BHK-21 cells inoculated with CDV, but not in the normal BHK-21 cells. The total RNA extracted from Vero cell culture inoculated with the virus were amplified by reverse transcription-polymerase chain reaction (RT-PCR), and two objective segments (324bp and 1053bp) were obtained. Thus, the isolated virus strain was proved to be CDV.