CAJ | 학술논문

目的探讨超声造影评价低温条件下兔肾皮质血流灌注变化的应用价值.方法将20只新西兰大白兔随机分为5组,每组4只:第1组为对照组,第2组-15℃～-23℃低温处理8h,第3组-15℃～-23℃低温处理4h,第4组-6℃～-14℃低温处理8h,第5组-6℃～-14℃低温处理4h.经耳缘静脉团注SonoVue(0.1 ml/kg)进行肾灌注造影检查,观察并采集注射造影剂后0～3 min的动态图像,并对获得的时间-强度曲线(TIC)进行分析,参数包括上升支斜率(A)、下降支斜率(α)、曲线下面积(AUC)、达峰时间(TTP)、达峰强度绝对值(DPI).结果与正常组比较,第2组和第4组的A、α明显减小(P＜0.05),AUC显著增大(P＜0.05),TTP明显延长(P＜0.05),第3组和第5组仅表现为A减小(P＜0.05),各组的DPI差异均无统计学意义；与第3组和第4组比较,第2组AUC增大(P＜0.05),TTP延长(P＜0.05).结论超声造影能较早反映低温条件下兔肾皮质血流动力学变化,对低温所致肾功能损害的早期诊断有一定的应用价值.
목적 탐토초성조영평개저온조건하토신피질혈류관주변화적응용개치.방법 장20지신서란대백토수궤분위5조,매조4지:제1조위대조조,제2조-15℃～-23℃저온처리8h,제3조-15℃～-23℃저온처리4h,제4조-6℃～-14℃저온처리8h,제5조-6℃～-14℃저온처리4h.경이연정맥단주SonoVue(0.1 ml/kg)진행신관주조영검사,관찰병채집주사조영제후0～3 min적동태도상,병대획득적시간-강도곡선(TIC)진행분석,삼수포괄상승지사솔(A)、하강지사솔(α)、곡선하면적(AUC)、체봉시간(TTP)、체봉강도절대치(DPI).결과 여정상조비교,제2조화제4조적A、α명현감소(P＜0.05),AUC현저증대(P＜0.05),TTP명현연장(P＜0.05),제3조화제5조부표현위A감소(P＜0.05),각조적DPI차이균무통계학의의；여제3조화제4조비교,제2조AUC증대(P＜0.05),TTP연장(P＜0.05).결론 초성조영능교조반영저온조건하토신피질혈류동역학변화,대저온소치신공능손해적조기진단유일정적응용개치.
Objective To explore the value of contrast-enhanced ultrasound(CEUS) in the evaluation of the renal cortical perfusion in rabbits in low temperature conditions.Methods 20 New Zealand rabbits were randomly divided into five groups,4 rabbits in each group:1) with the first group as the control group (under the conditions of room temperature) ; 2)The second group was treated under-15℃--23℃ for 8hours; 3)the third group was treated under-15℃--23℃ for 4 hours; 4)the fourth group was treated under-6℃--14℃ for 8 hours; 5) the fifth group was treated under-6℃--14℃ for 4 hours.SonoVue was injected via the ear vein at a dose of 0.1 ml/kg,the renal perfusion imaging was made,observing the dynamic image of 0-3 min after the injection and analyzing the time-intensity curves(TIC)obtained from the following parameters:the slope rate of ascending curve(A),the slope rate of descending curve(α),area under the curve (AUC),time to peak (TTP),and derived peak intensity(DPI).Results Compared with the control group,the A,α of the second and the fourth group were significantly decreased (P ＜ 0.05),while the AUC was significantly increased (P ＜ 0.05) and the TTP was significantly prolonged(P ＜0.05),for the third and the fifth group,only the A was decreased (P ＜0.05),and the DPI in each group showed no significant difference.Compared with the third and the fourth group,the AUC of the second group was increased and the TTP was prolonged (P ＜0.05).Conclusions Contrast-enhanced ultrasound can reflect the earlier renal cortical hemodynamic changes in rabbits in the low-temperature conditions,and is of great value for the early diagnosis of renal damage caused by low temperature.