CAJ | 학술논문

目的探讨受体血清(recipient serum,RS)“封闭”供肝与眼镜蛇毒因子(CVF)抑制肝移植超急性排斥反应(HAR)的协同作用.方法采用改良“双套管法”制作豚鼠→SD大鼠原位肝移植HAR模型.取豚鼠和SD大鼠各24只,分别作为供体和受体.供受体随机配对.移植前采集受体大鼠近交系其他个体血清,灭活补体备用.实验随机分为4组(n=6),受体血清(RS)实验组移植前用0.1％ RS的林格氏液(Ringer)“封闭”供肝,CVF实验组受体大鼠在术前24h经尾静脉注射CVF 50 μg/kg,另设RS+CVF联合实验组和Ringer液对照组.采用改良“双套管法”行豚鼠→SD大鼠原位肝移植；观察供肝植入后形态学改变、受体存活时间、移植肝病理损害程度和受体肝功能.结果各实验组大鼠异种供肝植入后均未见明显花斑样改变.各实验组大鼠移植后存活时间较对照组[(45.2±13.9)min]均明显延长(P＜0.05),其中RS+CVF组大鼠存活时间[(161.5±30.9) min]长于RS组[(88.1±19.7) min](P＜0.01)或CVF组[(125.2±25.5)min] (P＜0.05).各实验组大鼠血清丙氨酸转氨酶(ALT)均明显低于对照组[(126.1±23.3)U/L] (P＜0.05),RS+ CVF组ALT[(63.2±13.9)U/L]明显低于CVF组[(79.9±18.1)U/L](P＜0.05)或RS组[(106.1±19.3)U/L](P＜0.01).各实验组移植肝血栓、出血、水肿等病理损害(积分)均较对照组明显减轻(P＜0.05),其中RS+CVF组大鼠移植肝病理损害最轻(P＜0.05).结论受体血清“封闭”供肝与CVF对肝移植HAR均具有一定的抑制作用,两者具有协同作用.
목적 탐토수체혈청(recipient serum,RS)“봉폐”공간여안경사독인자(CVF)억제간이식초급성배척반응(HAR)적협동작용.방법 채용개량“쌍투관법”제작돈서→SD대서원위간이식HAR모형.취돈서화SD대서각24지,분별작위공체화수체.공수체수궤배대.이식전채집수체대서근교계기타개체혈청,멸활보체비용.실험수궤분위4조(n=6),수체혈청(RS)실험조이식전용0.1％ RS적림격씨액(Ringer)“봉폐”공간,CVF실험조수체대서재술전24h경미정맥주사CVF 50 μg/kg,령설RS+CVF연합실험조화Ringer액대조조.채용개량“쌍투관법”행돈서→SD대서원위간이식；관찰공간식입후형태학개변、수체존활시간、이식간병리손해정도화수체간공능.결과 각실험조대서이충공간식입후균미견명현화반양개변.각실험조대서이식후존활시간교대조조[(45.2±13.9)min]균명현연장(P＜0.05),기중RS+CVF조대서존활시간[(161.5±30.9) min]장우RS조[(88.1±19.7) min](P＜0.01)혹CVF조[(125.2±25.5)min] (P＜0.05).각실험조대서혈청병안산전안매(ALT)균명현저우대조조[(126.1±23.3)U/L] (P＜0.05),RS+ CVF조ALT[(63.2±13.9)U/L]명현저우CVF조[(79.9±18.1)U/L](P＜0.05)혹RS조[(106.1±19.3)U/L](P＜0.01).각실험조이식간혈전、출혈、수종등병리손해(적분)균교대조조명현감경(P＜0.05),기중RS+CVF조대서이식간병리손해최경(P＜0.05).결론 수체혈청“봉폐”공간여CVF대간이식HAR균구유일정적억제작용,량자구유협동작용.
Objective To investigate synergistic effect of donor livers blocked by recipient blood serum (RS) and cobra venom factor (CVF) treatment to inhibit hyperacute rejection (HAR) happened in liver xenotransplantation.Methods The SD rat blood serum was prepared for pre-perfusing the donor livers before experiment.24 pairs of guinea-pig (GP) and Sprague-Dawley (S.D.) rats were choiced respectively and pair-matched between GP donor and rat recipient randomly.Before transplantation,donor livers of GPs were pre-perfused by 0.5％ SD rat serum.Paired animals were divided into 4 groups randomly such as donor liver perfused by RS,recipient treated by CVF,RS+ CVF performed and Ringer solution as a control.The orthotopic liver xenotransplantations was performed with two-cuff technique.The survival time and liver function of recipients,morphological and pathological changes of rat livers were observed.Results There was no piebaldism change on the recipient liver from experimental group.The survival time of recipients from RS+CVF group [(161.5±30.9) min]was longer than that of control[(45.2 ± 13.9) min] and CVF[(125.2 ± 25.5) min] or RS groups [(88.1±19.7) min] (P＜0.05).The ALT in serum of recipients from RS+CVF [(63.2±13.9)U/L]was lower than that from congtrol group [(126.1±23.3)U/L](P＜0.01) and CVF group [(79.9±18.1)U/L](P＜0.05) or RS group [(106.1±19.3)U/L](P＜0.01) The histological damages including thrombosis,interstitial bleeding and edema of recipient liver from RS+CVF group were alleviated markebly than that of other groups (P＜0.05).Conclusion There was a synergistic effect to inhibit HAR happened in liver xenotransplantation by blocking the donor liver with recipient blood serum and CVF treatment significantly.