中华耳鼻咽喉头颈外科杂志
中華耳鼻嚥喉頭頸外科雜誌
중화이비인후두경외과잡지
CHINESE JOURNAL OF OTORHINOLARYNGOLOGY HEAD AND NECK SURGERY
2012年
10期
831-835
,共5页
刘洋%俞琳琳%韩军%程靖宁%杨大章
劉洋%俞琳琳%韓軍%程靖寧%楊大章
류양%유림림%한군%정정저%양대장
胆脂瘤,中耳%金属硫蛋白%白细胞介素1α%白细胞介素6%细胞系
膽脂瘤,中耳%金屬硫蛋白%白細胞介素1α%白細胞介素6%細胞繫
담지류,중이%금속류단백%백세포개소1α%백세포개소6%세포계
Cholesteatoma,middle ear%Metallothionein%Interleukin-1 alpha%Interleukin-6%Cell line
目的 观察金属硫蛋白2( metallothionein 2,MT-2)及白细胞介素1α(IL-1α)、白细胞介素6(IL-6)在中耳胆脂瘤组织中的表达及相关性,探讨其在中耳胆脂瘤发生发展过程中的作用.方法 采用免疫组化和反转录聚合酶链反应(RT-PCR)技术检测25例中耳胆脂瘤组织和7例正常外耳道皮肤组织中MT-2及IL-1α、IL-6蛋白和MT-2 mRNA的表达情况并分析其相关性.进一步以胆脂瘤鳞屑刺激人角质细胞系HaCaT,观察细胞MT-2 mRNA和蛋白质的表达变化.结果 MT-2、IL-1α及IL-6在中耳胆脂瘤中的表达明显高于正常外耳道皮肤,差异具有统计学意义(P值均＜0.05)；MT-2mRNA在中耳胆脂瘤和正常外耳道皮肤中的表达差异具有统计学意义(t=15.38,P＜0.05).中耳胆脂瘤组织中MT-2与IL-1α的表达呈正相关关系(r=0.856,P＜0.05),与IL-6的表达亦呈正相关关系(r=0.714,P＜0.05).在胆脂瘤鳞屑刺激下,HaCaT细胞MT-2的mRNA和蛋白质表达均有明显增强,其表达变化与鳞屑组织呈浓度依赖关系.结论 MT-2和IL-1α、IL-6的异常表达可能在中耳胆脂瘤的发生、发展过程中起一定的作用.胆脂瘤鳞屑组织可能通过促进MT-2的过量表达而参与了胆脂瘤上皮的过度增殖.
目的 觀察金屬硫蛋白2( metallothionein 2,MT-2)及白細胞介素1α(IL-1α)、白細胞介素6(IL-6)在中耳膽脂瘤組織中的錶達及相關性,探討其在中耳膽脂瘤髮生髮展過程中的作用.方法 採用免疫組化和反轉錄聚閤酶鏈反應(RT-PCR)技術檢測25例中耳膽脂瘤組織和7例正常外耳道皮膚組織中MT-2及IL-1α、IL-6蛋白和MT-2 mRNA的錶達情況併分析其相關性.進一步以膽脂瘤鱗屑刺激人角質細胞繫HaCaT,觀察細胞MT-2 mRNA和蛋白質的錶達變化.結果 MT-2、IL-1α及IL-6在中耳膽脂瘤中的錶達明顯高于正常外耳道皮膚,差異具有統計學意義(P值均＜0.05)；MT-2mRNA在中耳膽脂瘤和正常外耳道皮膚中的錶達差異具有統計學意義(t=15.38,P＜0.05).中耳膽脂瘤組織中MT-2與IL-1α的錶達呈正相關關繫(r=0.856,P＜0.05),與IL-6的錶達亦呈正相關關繫(r=0.714,P＜0.05).在膽脂瘤鱗屑刺激下,HaCaT細胞MT-2的mRNA和蛋白質錶達均有明顯增彊,其錶達變化與鱗屑組織呈濃度依賴關繫.結論 MT-2和IL-1α、IL-6的異常錶達可能在中耳膽脂瘤的髮生、髮展過程中起一定的作用.膽脂瘤鱗屑組織可能通過促進MT-2的過量錶達而參與瞭膽脂瘤上皮的過度增殖.
목적 관찰금속류단백2( metallothionein 2,MT-2)급백세포개소1α(IL-1α)、백세포개소6(IL-6)재중이담지류조직중적표체급상관성,탐토기재중이담지류발생발전과정중적작용.방법 채용면역조화화반전록취합매련반응(RT-PCR)기술검측25례중이담지류조직화7례정상외이도피부조직중MT-2급IL-1α、IL-6단백화MT-2 mRNA적표체정황병분석기상관성.진일보이담지류린설자격인각질세포계HaCaT,관찰세포MT-2 mRNA화단백질적표체변화.결과 MT-2、IL-1α급IL-6재중이담지류중적표체명현고우정상외이도피부,차이구유통계학의의(P치균＜0.05)；MT-2mRNA재중이담지류화정상외이도피부중적표체차이구유통계학의의(t=15.38,P＜0.05).중이담지류조직중MT-2여IL-1α적표체정정상관관계(r=0.856,P＜0.05),여IL-6적표체역정정상관관계(r=0.714,P＜0.05).재담지류린설자격하,HaCaT세포MT-2적mRNA화단백질표체균유명현증강,기표체변화여린설조직정농도의뢰관계.결론 MT-2화IL-1α、IL-6적이상표체가능재중이담지류적발생、발전과정중기일정적작용.담지류린설조직가능통과촉진MT-2적과량표체이삼여료담지류상피적과도증식.
Objective To detect the expression and distribution of metallothionein 2 (MT-2) in middle ear cholesteatoma,and to explore the relationship of MT-2 with interleukin 1α (IL-1α) and interleukin 6 (IL-6),and well as to explore the role of MT-2 in the pathogenetic mechanism of middle ear cholesteatoma.Methods Using the immunohistochemistry and reverse transcription polymerase chain reaction to examine the expressions of MT-2,IL-1 α and IL-6 protein and MT-2 mRNA in twenty-five middle ears' cholesteatoma and seven normal external ears' canal skin.The influence of cholesteatoma debris on the MT-2 mRNA and protein of HaCaT cell were further analyzed.Results According to immunohistochemistry research,the expressions of MT-2,IL-1 α and IL-6 were extremely higher in middle ear cholesteatoma than those in normal external ears' canal skin(P ＜ 0.05 ).The result of RT-PCR showed that there was significant difference between the expression of MT-2 mRNA of and middle ear cholesteatoma than those in normal external ear canal skin( t =15.38,P ＜ 0.05 ).There was a positive correlation between the expression of MT-2 and that of IL-1 α( r =0.856,P ＜ 0.05).There was also positive correlation between the expression of MT-2 and that of IL-6 ( r =0.714,P ＜ 0.05 ). MT-2 mRNA and protein of HaCaT cell significantly increased when exposed to cholesteatoma debris in a dose dependent manner.Conclusions MT-2,IL-1 αand IL-6 may play an important role in the pathogenetic process of middle ear cholesteatoma.Cholesteatoma debris may involve in the proliferation of middle ear cholesteatoma by activation of MT-2.
