中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2010年
6期
837-839
,共3页
蔡健华%赵任%万方军%刘坤%于颖彦%朱正纲
蔡健華%趙任%萬方軍%劉坤%于穎彥%硃正綱
채건화%조임%만방군%류곤%우영언%주정강
大肠癌%原位种植%肝转移%模型%动物
大腸癌%原位種植%肝轉移%模型%動物
대장암%원위충식%간전이%모형%동물
Colorectal cancer%Orthotopic implantation%liver met astases%Models,animal
目的 比较3种不同的人大肠癌裸鼠原位种植肝转移模型,建立稳定有效的人大肠癌裸鼠原位种植肝转移模型.方法 逐步传代法皮下成瘤传5代,采用84只BALB/C-nu/nu裸鼠,随机分为4组,结肠原位荷包缝合法和结肠原位纤维蛋白胶黏合法和结肠微注射1×106和1×105SW1116细胞法建立大肠癌裸鼠原位种植肝转移模型.各组裸鼠死亡后立即解剖观察原位以及肝脏、肺、脾脏、胰腺、肠系膜等脏器组织的转移并且进行苏木素-伊红(HE)染色进行镜下观察有无转移.结果 荷包缝合组和纤维蛋白胶组结肠原位种植肝转移成功率均为100%比微注射法50.0%和14.3%高,纤维蛋白胶法比其他两种方法所形成的结肠原位肿瘤和肝转移瘤均大而多.HE染色表明肝转移肿瘤性质与结肠原位种植瘤相同.结论 结肠原位纤维蛋白胶黏合法比较其他二种原位种植肝转移模型的手术成功率高,肝转移率高、操作简单.
目的 比較3種不同的人大腸癌裸鼠原位種植肝轉移模型,建立穩定有效的人大腸癌裸鼠原位種植肝轉移模型.方法 逐步傳代法皮下成瘤傳5代,採用84隻BALB/C-nu/nu裸鼠,隨機分為4組,結腸原位荷包縫閤法和結腸原位纖維蛋白膠黏閤法和結腸微註射1×106和1×105SW1116細胞法建立大腸癌裸鼠原位種植肝轉移模型.各組裸鼠死亡後立即解剖觀察原位以及肝髒、肺、脾髒、胰腺、腸繫膜等髒器組織的轉移併且進行囌木素-伊紅(HE)染色進行鏡下觀察有無轉移.結果 荷包縫閤組和纖維蛋白膠組結腸原位種植肝轉移成功率均為100%比微註射法50.0%和14.3%高,纖維蛋白膠法比其他兩種方法所形成的結腸原位腫瘤和肝轉移瘤均大而多.HE染色錶明肝轉移腫瘤性質與結腸原位種植瘤相同.結論 結腸原位纖維蛋白膠黏閤法比較其他二種原位種植肝轉移模型的手術成功率高,肝轉移率高、操作簡單.
목적 비교3충불동적인대장암라서원위충식간전이모형,건립은정유효적인대장암라서원위충식간전이모형.방법 축보전대법피하성류전5대,채용84지BALB/C-nu/nu라서,수궤분위4조,결장원위하포봉합법화결장원위섬유단백효점합법화결장미주사1×106화1×105SW1116세포법건립대장암라서원위충식간전이모형.각조라서사망후립즉해부관찰원위이급간장、폐、비장、이선、장계막등장기조직적전이병차진행소목소-이홍(HE)염색진행경하관찰유무전이.결과 하포봉합조화섬유단백효조결장원위충식간전이성공솔균위100%비미주사법50.0%화14.3%고,섬유단백효법비기타량충방법소형성적결장원위종류화간전이류균대이다.HE염색표명간전이종류성질여결장원위충식류상동.결론 결장원위섬유단백효점합법비교기타이충원위충식간전이모형적수술성공솔고,간전이솔고、조작간단.
Objective To establish and compare three orthotopic implantation nude mice models of colorectal cancer metastasis to liver. Methods Human SW1116 cell line was inoculated into 5 BALB/ C nude mice. Eighty-four nude mice were divided equally into pouch, fibrin glue, 1×106 cell and 1 × 105 cell microinjection groups. After animals were killed, the metastasis of cancer in situ, the liver, the lung, the spleen, the pancreas, and the mesentery was observed under the microscopy after HE staining. Results The metastasis rate in groups of pouch, fibrin glue, 1×1106 cell and 1×105 cell microinjection was 100% , 100% , 50. 0% and 14. 3% respectively. The liver metastasis tumor in fibrin glue group was bigger than other groups. HE staining indicated that the liver metastasis tumor was derived from colon tumor. Conclusion Among the three orthotopic implantation nude mice models of colorectal cancer metastasis to liver, the fibrin glue method has a high success rate of operation, a high metastasis rate of the liver, and is simple to manipulate.
