中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2012年
5期
963-965
,共3页
孙国柱%王目纲%刘性强%杨雷芳%韩仰军%赵宗茂
孫國柱%王目綱%劉性彊%楊雷芳%韓仰軍%趙宗茂
손국주%왕목강%류성강%양뢰방%한앙군%조종무
液压%脑损伤%模型,动物
液壓%腦損傷%模型,動物
액압%뇌손상%모형,동물
Fluid percussion%Brain injury%Model,animal
目的 建立不同分级液压冲击脑损伤动物模型,比较其病理学变化.方法 采用0.1、0.2、0.3MPa3的种不同打击压力,制作不同分级大鼠液压冲击脑损伤模型,观察生命体征变化、比较死亡率,观察造模后1、6、12、24h、3、7d时神经功能评分和脑水含量,比较其组织学演变.结果 应用0.1 MPa冲击,动物出现一过性呼吸减弱,死亡率2.08％,神经功能评分最低降至(7.17±0.75),但恢复较快,脑含水量最高达(81.12±0.03),脑损伤仅累及冲击伤处脑皮层的浅层；应用0.2 MPa冲击,动物呼吸暂停时间(10.88±2.69)s,死亡率4.17％,神经功能评分最低降至(4.83±0.75),脑水含量最高达(82.74±1.11),脑损伤深达海马结构；应用0.3 MPa冲击,大鼠呼吸暂停时间(20.60±3.02)s,死亡率16.67％,神经功能评分最低降至(2.67±0.52),而且恢复较慢,脑水含量最高达( 83.89±0.04),脑损伤深达海马下结构、脑干.结论轻、中、重3种不同损伤程度的脑损伤模型能够复制,具有损伤程度稳定且与人类脑损伤后病理变化相似的特点.
目的 建立不同分級液壓遲擊腦損傷動物模型,比較其病理學變化.方法 採用0.1、0.2、0.3MPa3的種不同打擊壓力,製作不同分級大鼠液壓遲擊腦損傷模型,觀察生命體徵變化、比較死亡率,觀察造模後1、6、12、24h、3、7d時神經功能評分和腦水含量,比較其組織學縯變.結果 應用0.1 MPa遲擊,動物齣現一過性呼吸減弱,死亡率2.08％,神經功能評分最低降至(7.17±0.75),但恢複較快,腦含水量最高達(81.12±0.03),腦損傷僅纍及遲擊傷處腦皮層的淺層；應用0.2 MPa遲擊,動物呼吸暫停時間(10.88±2.69)s,死亡率4.17％,神經功能評分最低降至(4.83±0.75),腦水含量最高達(82.74±1.11),腦損傷深達海馬結構；應用0.3 MPa遲擊,大鼠呼吸暫停時間(20.60±3.02)s,死亡率16.67％,神經功能評分最低降至(2.67±0.52),而且恢複較慢,腦水含量最高達( 83.89±0.04),腦損傷深達海馬下結構、腦榦.結論輕、中、重3種不同損傷程度的腦損傷模型能夠複製,具有損傷程度穩定且與人類腦損傷後病理變化相似的特點.
목적 건립불동분급액압충격뇌손상동물모형,비교기병이학변화.방법 채용0.1、0.2、0.3MPa3적충불동타격압력,제작불동분급대서액압충격뇌손상모형,관찰생명체정변화、비교사망솔,관찰조모후1、6、12、24h、3、7d시신경공능평분화뇌수함량,비교기조직학연변.결과 응용0.1 MPa충격,동물출현일과성호흡감약,사망솔2.08％,신경공능평분최저강지(7.17±0.75),단회복교쾌,뇌함수량최고체(81.12±0.03),뇌손상부루급충격상처뇌피층적천층；응용0.2 MPa충격,동물호흡잠정시간(10.88±2.69)s,사망솔4.17％,신경공능평분최저강지(4.83±0.75),뇌수함량최고체(82.74±1.11),뇌손상심체해마결구；응용0.3 MPa충격,대서호흡잠정시간(20.60±3.02)s,사망솔16.67％,신경공능평분최저강지(2.67±0.52),이차회복교만,뇌수함량최고체( 83.89±0.04),뇌손상심체해마하결구、뇌간.결론경、중、중3충불동손상정도적뇌손상모형능구복제,구유손상정도은정차여인류뇌손상후병리변화상사적특점.
Objective To establish the graded fluid percussion models of brain injury in rats and compare their pathological changes.Methods The fluid percussion models of brain injury in rats were established by using the improved device with three kinds of impact pressure of 0.1,0.2 and 0.3 MPa,and vital signs and mortality rate were observed.Behavior changes,brain water content and histological changes were observed by Shapira and Wahld method,dry-wet measure and light microscopy at 1st,6th,12th,24th h,and 3rd,7th day after operation respectively.Results The animals subject to impact pressure of 0.1 MPa showed temporary hypopnea with mortality rate of 2.08％,those to 0.2 MPa suffered apnoea of ( 10.88 ± 2.69) s with mortality rate of 4.17％,and those to 0.3 MPa suffered apnoea of (20.60 ± 3.02) s with mortality rate of 16.67％.Wih the impact pressure increased,nervous function score was minimumally decreased to (7.17 ±0.75) in0.1 MPa group,(4.83 ±0.75) in0.2 MPa group and (2.67 ±0.52) in 0.3 MPa group respectively,and recovered more slowly.Brain water content maximumly reached to (81.12 ± 0.03 ),( 82.74 ± 1.11 ) and ( 83.89 ± 0.04) at 24 h,respectively.The brain injury was involved in the outer layer of cerebral cortex,hippocampal formation and brain stem,and histological observation verified above findings.Conclusion Graded fluid percussion model of brain injury in rats can be produced stabily with the same pathological characteristics as those of human.
