中华检验医学杂志
中華檢驗醫學雜誌
중화검험의학잡지
CHINESE JOURNAL OF LABORATORY MEDICINE
2010年
10期
931-935
,共5页
肠球菌,粪%细菌蛋白质类%纤毛,细菌%生物膜%泌尿道感染
腸毬菌,糞%細菌蛋白質類%纖毛,細菌%生物膜%泌尿道感染
장구균,분%세균단백질류%섬모,세균%생물막%비뇨도감염
Enterococcus faecalis%Bacterial proteins%Fimbriae,bacterial%Biofilms%Urinary tract infections
目的研究粪肠球菌esp、gelE、ebpA基因和QS-fsr系统与粪肠球菌生物被膜形成的相关性.方法收集2007年10月至2008年6月从湘雅三医院临床尿路感染患者尿液及导尿管中分离的粪肠球菌24株,按生物被膜阳性粪肠球菌及相应的生物被膜阴性粪肠球菌分为2组.使用RT-PCR分别对生物被膜阳性组和阴性组细菌的esp、ebpA、gelE和fsrB基因荧光强度比进行检测;利用real-time PCR分别检测生物被膜阳性组和阴性组细菌的esp、ebpA、gelE和fsrB 4种与生物被膜形成相关的基因的表达,并通过2-△△Ct方法进行相对定量.分析粪肠球菌esp、gelE、ebpA基因及QS-fsr系统与生物被膜形成的关系.结果 real-time PCR检测显示生物被膜阳性组粪肠球菌esp和ebpA表达量分别是生物被膜阴性组的298倍和59倍,生物被膜阳性组粪肠球菌gelE和fsrB表达量分别是生物被膜阴性组的1/244和1/249.生物被膜组和浮游生物组细菌esp、ebpA、gelE和fsB基因的荧光强度比,差异无统计学意义(秩和检验^值分别为92、79、42和34,P均＞0.05).结论 esp、ebpA、gelE和fsrB基因与粪肠球菌生物被膜形成密切相关.esp和ebpA能促进生物被膜形成,gelE和fsB能抑制生物被膜形成.提示fsr系统能调节esp、ebpA和gelE基因的表达.
目的研究糞腸毬菌esp、gelE、ebpA基因和QS-fsr繫統與糞腸毬菌生物被膜形成的相關性.方法收集2007年10月至2008年6月從湘雅三醫院臨床尿路感染患者尿液及導尿管中分離的糞腸毬菌24株,按生物被膜暘性糞腸毬菌及相應的生物被膜陰性糞腸毬菌分為2組.使用RT-PCR分彆對生物被膜暘性組和陰性組細菌的esp、ebpA、gelE和fsrB基因熒光彊度比進行檢測;利用real-time PCR分彆檢測生物被膜暘性組和陰性組細菌的esp、ebpA、gelE和fsrB 4種與生物被膜形成相關的基因的錶達,併通過2-△△Ct方法進行相對定量.分析糞腸毬菌esp、gelE、ebpA基因及QS-fsr繫統與生物被膜形成的關繫.結果 real-time PCR檢測顯示生物被膜暘性組糞腸毬菌esp和ebpA錶達量分彆是生物被膜陰性組的298倍和59倍,生物被膜暘性組糞腸毬菌gelE和fsrB錶達量分彆是生物被膜陰性組的1/244和1/249.生物被膜組和浮遊生物組細菌esp、ebpA、gelE和fsB基因的熒光彊度比,差異無統計學意義(秩和檢驗^值分彆為92、79、42和34,P均＞0.05).結論 esp、ebpA、gelE和fsrB基因與糞腸毬菌生物被膜形成密切相關.esp和ebpA能促進生物被膜形成,gelE和fsB能抑製生物被膜形成.提示fsr繫統能調節esp、ebpA和gelE基因的錶達.
목적연구분장구균esp、gelE、ebpA기인화QS-fsr계통여분장구균생물피막형성적상관성.방법수집2007년10월지2008년6월종상아삼의원림상뇨로감염환자뇨액급도뇨관중분리적분장구균24주,안생물피막양성분장구균급상응적생물피막음성분장구균분위2조.사용RT-PCR분별대생물피막양성조화음성조세균적esp、ebpA、gelE화fsrB기인형광강도비진행검측;이용real-time PCR분별검측생물피막양성조화음성조세균적esp、ebpA、gelE화fsrB 4충여생물피막형성상관적기인적표체,병통과2-△△Ct방법진행상대정량.분석분장구균esp、gelE、ebpA기인급QS-fsr계통여생물피막형성적관계.결과 real-time PCR검측현시생물피막양성조분장구균esp화ebpA표체량분별시생물피막음성조적298배화59배,생물피막양성조분장구균gelE화fsrB표체량분별시생물피막음성조적1/244화1/249.생물피막조화부유생물조세균esp、ebpA、gelE화fsB기인적형광강도비,차이무통계학의의(질화검험^치분별위92、79、42화34,P균＞0.05).결론 esp、ebpA、gelE화fsrB기인여분장구균생물피막형성밀절상관.esp화ebpA능촉진생물피막형성,gelE화fsB능억제생물피막형성.제시fsr계통능조절esp、ebpA화gelE기인적표체.
Objective To investigate the association of esp, gelE, ebpA and QS-fsr system and biofilm formation in Enterococcus faecalis. Methods Totally 24 isolates of Enterococcus faecalis were collected from urine and catheter of clinical urine tract infection patients in Third Xiangya Hospital from Oct. 2007 to Jun. 2008, and were divided into biofilm group and non-biofilm group. The luminance ratios of esp, gelE, ebpA and fsrrB of Enterococcus faecalis in biofilm group and non-biofilm group were detected by RT-PCR. And the expression of esp, gelE, ebpA, fsrrB genes in different groups were detected by real-time PCR and were relatively quantitated through 2-△△Ct method. Moreover, the relevancies between that fourgenes and biofilm formation in Enterococcus faecalis were analyzed respectively. Results The expression of esp and ebpA in biofilm group were 298 times and 59 times more than the non-biofilm group. The expression level ofgelE and fsrB in biofilm group were 1/244 and 1/249 times less than the non-biofilm group, and the luminance ratios of esp, gelE, ebpA and fsrB were not significant between the two groups (rank sum was 92,79, 42 and 34 respectively,all P ＞ 0. 05 ). Conclusions The results showed that the biofilm formation in Enterococcus faecalis was promoted by esp and ebpA, and was inhabited by gelE and fsrB, which suggested that the expression of esp, ebpA and gelE genes was regulated by fsr system.
