CAJ | 학술논문

利用绿色荧光蛋白基因结合鼠Talin基因表达技术及水稻转基因技术,在未成熟花粉发育期(即生殖细胞在形成后从靠壁部位移向中央部位的阶段)的水稻(Oryza sativa L.)内发现了一系列前人未曾报道过的微丝骨架的形成和多变过程.在这一发育阶段,未成熟花粉内的生殖细胞呈圆形,中央部位存有一个大液泡,大量微丝在细胞的中央胞质内形成.微丝首先在营养核的核膜表面形成两个集结中心,中心内的微丝呈短粗状.尔后,中心微丝不断延长,最终在细胞中央的胞质内形成一个非常复杂的类似多个纺锤体结合在一起的网络结构.这一网络的中间部位经常包围着营养核和生殖细胞,网络的部分微丝则与存在周缘细胞质(或称周质)的微丝网络形成连接,在连接点部位则形成一些由微丝环状组成的结构.未成熟花粉中央的微丝网络可能与营养核和生殖细胞在未成熟花粉内的运动有密切关系.
이용록색형광단백기인결합서Talin기인표체기술급수도전기인기술,재미성숙화분발육기(즉생식세포재형성후종고벽부위이향중앙부위적계단)적수도(Oryza sativa L.)내발현료일계렬전인미증보도과적미사골가적형성화다변과정.재저일발육계단,미성숙화분내적생식세포정원형,중앙부위존유일개대액포,대량미사재세포적중앙포질내형성.미사수선재영양핵적핵막표면형성량개집결중심,중심내적미사정단조상.이후,중심미사불단연장,최종재세포중앙적포질내형성일개비상복잡적유사다개방추체결합재일기적망락결구.저일망락적중간부위경상포위착영양핵화생식세포,망락적부분미사칙여존재주연세포질(혹칭주질)적미사망락형성련접,재련접점부위칙형성일사유미사배상조성적결구.미성숙화분중앙적미사망락가능여영양핵화생식세포재미성숙화분내적운동유밀절관계.
Green fluorescent protein (GFP) fused to the F-actin binding domain of mouse talin labels the actin cytoskeleton in the immature pollen of stable transformed rice (Oryza sativa L.) plants.Actin microfilaments could be visualized only in the late-developmental stage of the immature pollen.During this developmental stage,microfilaments,initially composed of very short fibrils,develop into a very complex and novel network that sometimes totally and sometimes partially encloses the vegetative nucleus and the spherical shaped generative cell in the central cytoplasm of the immature pollen.The behavior of the actin microfilamentous structure throughout the late-developmental stage of the immature pollen is extremely dynamic,and the likelihood of this structure in generating forces for vegetative nucleus and generative cell movement in the immature pollen has been discussed.No actin filaments were visualized in the spherical generative cells.