CAJ | 학술논문

分析了水稻籼粳交加倍单倍体(DH)群体中影响白背飞虱抗虫性和感虫性的QTL.虽然DH株系的亲本窄叶青8号和京系17没有拒取食抗性,但是白背飞虱在6个DH株系中的取食受到了强烈的抑制,可能属超亲分离.在第3染色体的粳型片段中检测到1个影响蜜露分泌的微效QTL.粳稻亲本京系17具有杀卵抗性.DH株系中的杀卵特性是通过叶鞘上杀卵反应产生的坏死症状表现的.在DH株系分蘖早期和中期,将4个杀卵作用的QTL定位在第1、2、6和8染色体的粳型片段上.出现在分蘖中期的另一个QTL被定位在第9染色体的籼型片段上.在分蘖盛期至孕穗期,杀卵位点减少至2个.整个试验期间对每个DH株系的最高杀卵级别的分析显示,在染色体2、6和9上共有4个QTL.两个主效QTL位于近邻第6染色体的粳型片段.在第1、3和5染色体上检测到3个影响第2代白背飞虱若虫密度的QTL.第3染色体上起主要作用的QTL源自粳稻亲本;第5染色体上的微效QTL源自籼稻亲本.两个白背飞虱为害的QTL位于第8和第10染色体的籼型片段,另一个QTL位于第3染色体的粳型片段.这些QTL被认为与水稻品种对白背飞虱田间抗性表达有关.
분석료수도선갱교가배단배체(DH)군체중영향백배비슬항충성화감충성적QTL.수연DH주계적친본착협청8호화경계17몰유거취식항성,단시백배비슬재6개DH주계중적취식수도료강렬적억제,가능속초친분리.재제3염색체적갱형편단중검측도1개영향밀로분비적미효QTL.갱도친본경계17구유살란항성.DH주계중적살란특성시통과협초상살란반응산생적배사증상표현적.재DH주계분얼조기화중기,장4개살란작용적QTL정위재제1、2、6화8염색체적갱형편단상.출현재분얼중기적령일개QTL피정위재제9염색체적선형편단상.재분얼성기지잉수기,살란위점감소지2개.정개시험기간대매개DH주계적최고살란급별적분석현시,재염색체2、6화9상공유4개QTL.량개주효QTL위우근린제6염색체적갱형편단.재제1、3화5염색체상검측도3개영향제2대백배비슬약충밀도적QTL.제3염색체상기주요작용적QTL원자갱도친본;제5염색체상적미효QTL원자선도친본.량개백배비슬위해적QTL위우제8화제10염색체적선형편단,령일개QTL위우제3염색체적갱형편단.저사QTL피인위여수도품충대백배비슬전간항성표체유관.
Quantitative trait loci (QTLs) affecting the whitebacked planthopper (WBPH) resistance and susceptibility in an indica/japonica doubled halpoid (DH) rice population were analyzed. Although the parents of DH lines, Zhaiyeqing 8 (ZYQ-8, indica) and Jingxi 17 (JX-17, japonica), had no sucking inhibitory resistance, WBPH sucking was strongly suppressed in six DH lines for possible transgressive segregation. A minor QTL affecting honeydew excretion was detected at a japonica segment of chromosome 3. Japonica parent JX-17 had ovicidal resistance to WBPH. Ovicidal trait in DH lines was phenotyped by necrotic symptoms on the leaf sheaths due to ovicidal response. Four ovicidal QTLs were located on japonica segments of chromosomes 1, 2, 6, and 8 in DH lines at early- and mid-tillering stages. One more QTL appearing at mid-tillering stage was located on an indica segment of chromosome 9. The ovicidal loci reduced to two at the maximum tillering to booting stage. Analysis based on the maximum score of ovicidal symptom for each DH line throughout the experimental period revealed four QTLs on chromosomes 2, 6 and 9. Two major QTLs were located closely on japonica segments of chromosome 6. Three QTLs affecting the density of the second-generation WBPH nymphs were detected on chromosomes 1, 3 and 5. A main-effect QTL on chromosome 3 was derived from the japonica parent. A minor QTL on chromosome 5 came from the indica parent. Two QTLs for WBPH damages were located on indica segments of chromosomes 8 and 10, and the other QTL on a japonica segment of chromosome 3. These QTLs were considered to be associated with the expression of field resistance of rice varieties to WBPH.