CAJ | 학술논문

在黑暗条件下筛选拟南芥滞绿突变体时,得到一株突变体,dst6,表现出典型的油菜素突变体的特征. 遗传分析表明其是单基因隐性突变. 利用简单序列多态性标记,将其定位于2号染色体的底部. 粗定位的结果和形态学特征表明dst6可能是一个det2等位基因突变株. 通过对dst6中DET2基因的测序分析,证明了dst6可能是一个新的det2等位基因突变株.
재흑암조건하사선의남개체록돌변체시,득도일주돌변체,dst6,표현출전형적유채소돌변체적특정. 유전분석표명기시단기인은성돌변. 이용간단서렬다태성표기,장기정위우2호염색체적저부. 조정위적결과화형태학특정표명dst6가능시일개det2등위기인돌변주. 통과대dst6중DET2기인적측서분석,증명료dst6가능시일개신적det2등위기인돌변주.
In an attempt to screen stay-green mutants in Arabidopsis in the absence of light,a mutant displaying the characteristics of brassinosteroids mutants was isolated. This mutant,dst6,carries a single recessive mutation. Using molecular genetic markers,the mutation was mapped to the bottom of chromosome Ⅱ,which is around the region of DET2,one of the genes known to cause brassinosteroids mutation. The analysis of cDNA and genomic sequence of DET2 in dst6 revealed a frame-shifting insertion. These results suggest that dst6 is a putative new allele of det2.