CAJ | 학술논문

研究证明,传统膜片钳放大器在电流钳模式下记录到的快速电压信号会存在失真,且造成失真的根本原因是由于膜片钳的探头电路设计.为了克服这些缺陷重新设计了一种探头,新探头电路不仅能像传统的电压跟随器一样测量瞬态电压,而且适用于传统的电压钳工作模式.此外,一种命名为电压钳控制的电流钳技术被应用来改进传统的膜片钳放大器.用可变的低通滤波器来调整电压钳模块的响应速度,从而在实现膜电位钳位的同时准确记录快速电压信号.桥平衡电路用来消除命令电流流过串联电阻时带来的电压误差.而传统膜片钳中的快电容补偿环节则被改进用来补偿电极分布电容和探头放大器输入电容并提高电流钳模式下系统的响应速度.细胞模型实验结果表明,改进后的膜片钳放大器能够满足电生理研究中快速电位变化测量的需要.
연구증명,전통막편겸방대기재전류겸모식하기록도적쾌속전압신호회존재실진,차조성실진적근본원인시유우막편겸적탐두전로설계.위료극복저사결함중신설계료일충탐두,신탐두전로불부능상전통적전압근수기일양측량순태전압,이차괄용우전통적전압겸공작모식.차외,일충명명위전압겸공제적전류겸기술피응용래개진전통적막편겸방대기.용가변적저통려파기래조정전압겸모괴적향응속도,종이재실현막전위겸위적동시준학기록쾌속전압신호.교평형전로용래소제명령전류류과천련전조시대래적전압오차.이전통막편겸중적쾌전용보상배절칙피개진용래보상전겁분포전용화탐두방대기수입전용병제고전류겸모식하계통적향응속도.세포모형실험결과표명,개진후적막편겸방대기능구만족전생리연구중쾌속전위변화측량적수요.
Previous research rcvealed that distortion is detected in transient voltage signal recorded with traditional patch clamp amplifier under current clamp mode, which is essentially resulted by electronic design of the headstage of the patch clamp. A new kind of headstage is designed to modify the defect, the circuit of which not only measures the transient potentials as the classical voltage follower does but also is quite suitable for the standard voltage-clamp mode. Furthermore, the technique of voltage-clamp-controlled current-clamp is applied for modifying the conventional patch-clamp amplifier, the variable low-pass filter is added into the circuit to reduce the response speed of voltage-clamp module, thus the transient potentials changes can be measured while membrane potential is kept at a constant value. Bridge balance circuitry is designed to eliminate the voltage drop while the variable current injected into the electrode. And fast capacitance compensation stage of conventional PCA is modified to nentralize the capacitance and accelerate system response speed for current-clamp mode. The experirnental results on cell model demonstrate that modified PCA meets the rcquirement of monitoring transient potential changes in electrophysiology research.