食品科学
食品科學
식품과학
FOOD SCIENCE
2009年
21期
338-342
,共5页
曹晋宜%韩军丽%王友湘%陈庆森%阎亚丽
曹晉宜%韓軍麗%王友湘%陳慶森%閻亞麗
조진의%한군려%왕우상%진경삼%염아려
瑞士乳杆菌%细胞因子%肠道黏膜
瑞士乳桿菌%細胞因子%腸道黏膜
서사유간균%세포인자%장도점막
Lactobacillus helveticus%cytokine%intestinal mucosa
了解服用益生菌小鼠肠道黏膜和组织中细胞因子的变化情况,对阐明其对小鼠肠道黏膜免疫的影响具有重要的价值.本实验将60只小鼠随机分成4组:日食对照组、生理盐水对照组、瑞士乳杆菌灌喂组和大肠杆菌对照组,连续灌喂15d,采集小鼠小肠利用ELISA法测得各组小鼠肠道黏膜和肠道组织中细胞因子的含量,并作分析.研究结果证实,与对照组相比,瑞士乳杆菌灌胃组小鼠肠黏膜和组织中4种细胞因了明显升高,且存在显著性筹异(P<0.05):白介素-2IL-2在3d前达到最大值、白介素-4(1L-4)在5d达到最高点、白介素-6(IL-6)在5d达到顶峰、干扰素-γ(IFN-γ)在3d前达到最大值;与对照组相比,瑞士乳杆菌灌胃组小鼠Th1型和Th2型T细胞的不存在显著性差异(P>0.05),肠道免疫功能未出现异常,而大肠杆菌灌胃组Th1型和Th2型T细胞平衡被打乱,免疫功能出现异常.
瞭解服用益生菌小鼠腸道黏膜和組織中細胞因子的變化情況,對闡明其對小鼠腸道黏膜免疫的影響具有重要的價值.本實驗將60隻小鼠隨機分成4組:日食對照組、生理鹽水對照組、瑞士乳桿菌灌餵組和大腸桿菌對照組,連續灌餵15d,採集小鼠小腸利用ELISA法測得各組小鼠腸道黏膜和腸道組織中細胞因子的含量,併作分析.研究結果證實,與對照組相比,瑞士乳桿菌灌胃組小鼠腸黏膜和組織中4種細胞因瞭明顯升高,且存在顯著性籌異(P<0.05):白介素-2IL-2在3d前達到最大值、白介素-4(1L-4)在5d達到最高點、白介素-6(IL-6)在5d達到頂峰、榦擾素-γ(IFN-γ)在3d前達到最大值;與對照組相比,瑞士乳桿菌灌胃組小鼠Th1型和Th2型T細胞的不存在顯著性差異(P>0.05),腸道免疫功能未齣現異常,而大腸桿菌灌胃組Th1型和Th2型T細胞平衡被打亂,免疫功能齣現異常.
료해복용익생균소서장도점막화조직중세포인자적변화정황,대천명기대소서장도점막면역적영향구유중요적개치.본실험장60지소서수궤분성4조:일식대조조、생리염수대조조、서사유간균관위조화대장간균대조조,련속관위15d,채집소서소장이용ELISA법측득각조소서장도점막화장도조직중세포인자적함량,병작분석.연구결과증실,여대조조상비,서사유간균관위조소서장점막화조직중4충세포인료명현승고,차존재현저성주이(P<0.05):백개소-2IL-2재3d전체도최대치、백개소-4(1L-4)재5d체도최고점、백개소-6(IL-6)재5d체도정봉、간우소-γ(IFN-γ)재3d전체도최대치;여대조조상비,서사유간균관위조소서Th1형화Th2형T세포적불존재현저성차이(P>0.05),장도면역공능미출현이상,이대장간균관위조Th1형화Th2형T세포평형피타란,면역공능출현이상.
In order to study the effect of Lactobacillus helveticus on intestinal immunity in mice, cytokines in intestinal mucosa and tissue of mice were analyzed. Totally 60 mice were randomly divided into four groups: normal diet group, normal saline control group, Lactobacillus helveticus group, and E. coli group. All mice except the normal diet control group were orally administered corresponding probiotics for continuously15 days, and then cytokines in intestine mucosa and tissue of mice were detected using ELISA to analyze immunoregulatory function of Lactobacillus helveticus. Lactobacillus helveticus-administered mice had a significantly higher level of four cytokines when compared to control groups (P < 0.05). The highest level of cytokines was observed at 3 days post-administration for IL-2 and IFN-γ, and at 5 days post-administration for IL-4 and IL-6. Meanwhile, no obvious change in population of Th1 and Th2 type T cells in intestinal mucosa and intestinal tissue of Lactobacillus helveticus-administered mice was observed; in contrast, the balance of Th1 and Th2 type T cells in E. coli-administered mice was disturbed, which revealed that Lactobacillus helveticus had a protective effect on intestinal immune.
