CAJ | 학술논문

设计旨在研制一种新型的手持式PCR扩增仪,利用微电子机械系统(MEMS)技术制作PCR微加热芯片,采用聚二甲基硅氧烷(PDMS)材料制作一次性使用的微反应腔阵列,以C8051F020微控制器为核心搭建一个可以同时进行多路扩增的温控系统.它从根本上克服了传统台式PCR扩增仪的缺点,具有体积小、重量轻、反应速率快、节省反应试剂等诸多优点.
설계지재연제일충신형적수지식PCR확증의,이용미전자궤계계통(MEMS)기술제작PCR미가열심편,채용취이갑기규양완(PDMS)재료제작일차성사용적미반응강진렬,이C8051F020미공제기위핵심탑건일개가이동시진행다로확증적온공계통.타종근본상극복료전통태식PCR확증의적결점,구유체적소、중량경、반응속솔쾌、절성반응시제등제다우점.
A new type handheld PCR amplification is designed, in which PCR microchip is fabricated with MEMS technology and the one-off microarray is made of PDMS material. C8051F020 microcontroller is made as a core to build a temperature-controlled system, which can carry out several amplifications synchronously. It can overcome the defects of traditional desktop PCR amplification and its advantages include small volume,light weight, fast reaction velocity and saving reaction reagent.