高等学校化学学报
高等學校化學學報
고등학교화학학보
CHEMICAL JOURNAL OF CHINESE UNIVERSITIES
2010年
3期
484-487
,共4页
王健生%张佳%吴文安%段小艺%王嗣岑%张明鑫%周苏娜%莫非%徐怡庄%石景森%吴瑾光
王健生%張佳%吳文安%段小藝%王嗣岑%張明鑫%週囌娜%莫非%徐怡莊%石景森%吳瑾光
왕건생%장가%오문안%단소예%왕사잠%장명흠%주소나%막비%서이장%석경삼%오근광
胆囊%胆囊癌%细胞膜%胆囊癌细胞株%红外光谱
膽囊%膽囊癌%細胞膜%膽囊癌細胞株%紅外光譜
담낭%담낭암%세포막%담낭암세포주%홍외광보
Gallbladder%Gallbladder cancer%Plasma membrane%Gallbladder cancer cell line%FTIR
研究了不同病理生理状态的胆囊组织、胆囊组织细胞膜及胆囊癌细胞株的傅里叶变换红外光谱的相关性.结果显示,与正常组织比较,胆囊癌组织中脂质相关谱带(2955,2920,2870,2850和1740 cm~(-1))与蛋白相关谱带(1550 cm~(-1))强度降低;胆囊癌细胞株中脂质相关谱带与蛋白相关谱带(1550 cm~(-1))强度明显增加;胆囊癌组织细胞膜中脂质相关谱带强度明显增加;核酸相关吸收谱带强度变化在胆囊癌组织和胆囊癌细胞株中一致,在细胞膜中消失;I_(1460)/I_(1400)比值在组织、细胞株和细胞膜层面变化一致,与正常组织相比均有所减少.结果表明,与胆囊癌组织相比,胆囊癌细胞株和胆囊癌组织细胞膜的红外光谱发生了显著变化: 癌组织的脂类和蛋白质的相对含量降低;癌细胞株的脂质和蛋白相对含量增加;胆囊癌组织细胞膜的脂质和蛋白相对含量升高.从组织、细胞和细胞膜3个层面阐述了癌变的分子光谱变化.I_(1460)/I_(1400)比值的变化在组织、细胞株和细胞膜层面变化一致,对此指标的深入研究有望获得一个良好的临床诊断指标.
研究瞭不同病理生理狀態的膽囊組織、膽囊組織細胞膜及膽囊癌細胞株的傅裏葉變換紅外光譜的相關性.結果顯示,與正常組織比較,膽囊癌組織中脂質相關譜帶(2955,2920,2870,2850和1740 cm~(-1))與蛋白相關譜帶(1550 cm~(-1))彊度降低;膽囊癌細胞株中脂質相關譜帶與蛋白相關譜帶(1550 cm~(-1))彊度明顯增加;膽囊癌組織細胞膜中脂質相關譜帶彊度明顯增加;覈痠相關吸收譜帶彊度變化在膽囊癌組織和膽囊癌細胞株中一緻,在細胞膜中消失;I_(1460)/I_(1400)比值在組織、細胞株和細胞膜層麵變化一緻,與正常組織相比均有所減少.結果錶明,與膽囊癌組織相比,膽囊癌細胞株和膽囊癌組織細胞膜的紅外光譜髮生瞭顯著變化: 癌組織的脂類和蛋白質的相對含量降低;癌細胞株的脂質和蛋白相對含量增加;膽囊癌組織細胞膜的脂質和蛋白相對含量升高.從組織、細胞和細胞膜3箇層麵闡述瞭癌變的分子光譜變化.I_(1460)/I_(1400)比值的變化在組織、細胞株和細胞膜層麵變化一緻,對此指標的深入研究有望穫得一箇良好的臨床診斷指標.
연구료불동병리생리상태적담낭조직、담낭조직세포막급담낭암세포주적부리협변환홍외광보적상관성.결과현시,여정상조직비교,담낭암조직중지질상관보대(2955,2920,2870,2850화1740 cm~(-1))여단백상관보대(1550 cm~(-1))강도강저;담낭암세포주중지질상관보대여단백상관보대(1550 cm~(-1))강도명현증가;담낭암조직세포막중지질상관보대강도명현증가;핵산상관흡수보대강도변화재담낭암조직화담낭암세포주중일치,재세포막중소실;I_(1460)/I_(1400)비치재조직、세포주화세포막층면변화일치,여정상조직상비균유소감소.결과표명,여담낭암조직상비,담낭암세포주화담낭암조직세포막적홍외광보발생료현저변화: 암조직적지류화단백질적상대함량강저;암세포주적지질화단백상대함량증가;담낭암조직세포막적지질화단백상대함량승고.종조직、세포화세포막3개층면천술료암변적분자광보변화.I_(1460)/I_(1400)비치적변화재조직、세포주화세포막층면변화일치,대차지표적심입연구유망획득일개량호적림상진단지표.
12 Cancer tissue samples, 14 inflammatory samples and 10 normal samples of gallbladder and their corresponding tissue membranes and gallbladder cancer cell lines were observed via FTIR. Compared to normal gallbladder tissue, the intensities of bands, related to lipids, at 2955, 2920, 2870, 2850 and 1740 cm~(-1), decreased for gallbladder cancer tissue, the intensity of bands, related to protein, at 1550 cm~(-1) decreased for gallbladder cancer tissue;the intensities of lipids bands and protein bands increased for gallbladder cancer cell line obviously;the intensities of lipids related bands also increased for gallbladder cancer tissue membrane;the intensity of band related to nucleic acid for gallbladder cancer tissue is similar to that for gallbladder cancer cell line and the band disappeared for gallbladder cancer tissue plasma membrane;the ratio of I_(1460)/I_(1400) changed uniformity for tissue, cell line and tissue plasma membrane, which is smaller for cancer ones and bigger for normal ones. These results indicate that compared with those of gallbladder cancer tissue, the FTIR spectra changed a lot for gallbladder cancer cell lines and gallbladder cancer tissue plasma membrane. Protein and lipids relatively decreased in gallbladder cancer tissues but increased in gallbladder cancer cell line and gallbladder cancer tissue plasma membrane. The paper expounds FTIR variation for carcinogenesis from tissue, cell line and tissue plasma membrane. The ratio of I_(1460)/I_(1400) changed uniformity for tissue, cell line and tissue plasma membrane, more studies may get a fine clinical diagnosis index.
