中华胰腺病杂志
中華胰腺病雜誌
중화이선병잡지
CHINESE JOURNAL OF PANCREATOLOGY
2010年
3期
180-183
,共4页
唐丙喜%邓芝云%孔祥才%陈嘉屿%张方信
唐丙喜%鄧芝雲%孔祥纔%陳嘉嶼%張方信
당병희%산지운%공상재%진가서%장방신
胰腺炎,急性坏死性%大黄甘草汤%肺损伤%Toll样受体4%细胞因子类
胰腺炎,急性壞死性%大黃甘草湯%肺損傷%Toll樣受體4%細胞因子類
이선염,급성배사성%대황감초탕%폐손상%Toll양수체4%세포인자류
Pancreatitis,acute necrotizing%Dahuang gancao decoction%Lung injury%Toil-like receptor 4%Cytokines
目的 观察大黄甘草汤对急性坏死性胰腺炎(ANP)大鼠并发肺损伤的治疗效果,探讨其作用机制.方法 90只Wistar大鼠按完全随机法分为假手术组、ANP组和大黄甘草汤治疗(治疗)组,每组30只.采用逆行胰胆管注射4%牛磺胆酸钠方法制备ANP模型.治疗组在制模后给予大黄甘草汤(0.25 g/ml)0.6 ml/100 g体重灌胃,1次/12 h;假手术组与ANP组予等量生理盐水灌胃.术后6、12、24 h分批处死大鼠,取胰腺及肺组织行病理学检查并评分;测血清及肺组织IL-6、IL-10、TNF-α水平;免疫组化法检测肺组织Toll样受体4(TLR4)的表达量.结果 制模后12 h,假手术组、ANP组和治疗组的血IL-6水平分别为(14.4±4.0)pg/ml、(171.4±41.3)pg/ml、(156.9±34.7)pg/ml,IL-10水平为(13.7±4.5)pg/ml、(120.5±23.7)pg/ml、(148.3±44.4)pg/ml,TNF-α水平为(22.4±4.7)pg/ml、(261.3±51.4)pg/ml、(235.3±45.9)pg/ml;肺组织IL-6水平为(257.3±55.9)pg/g、(2578.3±403.0)pg/g、(2370.0±491.0)pg/g,IL-10水平为(80.8±20.8)Pg/g、(642.0±107.3)pg/g、(695.3±151.7)Pg/g,TNF-α水平为(207.6±98.6)pg/g、(1769.1±635.6)Pg/g、(1401.1±450.5)pg/g;胰腺病理评分为0、7.0±1.3、6.3±1.0;肺脏病理评分为0、6.3±1.4、5.6±1.0;肺组织TLR4表达量为0.09 ±0.03、0.59±0.09、0.52±0.08.ANP组和治疗组的上述指标均显著高于假手术组(P值均＜0.01);除IL-10外,治疗组的指标又显著低于ANP组(P值均＜0.05).肺组织病理学损伤与胰腺组织损伤呈正相关(r=0.807,P＜0.01),与TLR4表达水平亦呈正相关(r=0.519,P＜0.01).结论 大黄甘草汤可快速改善ANP并发的肺损伤,其机制可能与抑制TLR4的表达、降低IL-6和TNF-α、升高IL-10水平有关.
目的 觀察大黃甘草湯對急性壞死性胰腺炎(ANP)大鼠併髮肺損傷的治療效果,探討其作用機製.方法 90隻Wistar大鼠按完全隨機法分為假手術組、ANP組和大黃甘草湯治療(治療)組,每組30隻.採用逆行胰膽管註射4%牛磺膽痠鈉方法製備ANP模型.治療組在製模後給予大黃甘草湯(0.25 g/ml)0.6 ml/100 g體重灌胃,1次/12 h;假手術組與ANP組予等量生理鹽水灌胃.術後6、12、24 h分批處死大鼠,取胰腺及肺組織行病理學檢查併評分;測血清及肺組織IL-6、IL-10、TNF-α水平;免疫組化法檢測肺組織Toll樣受體4(TLR4)的錶達量.結果 製模後12 h,假手術組、ANP組和治療組的血IL-6水平分彆為(14.4±4.0)pg/ml、(171.4±41.3)pg/ml、(156.9±34.7)pg/ml,IL-10水平為(13.7±4.5)pg/ml、(120.5±23.7)pg/ml、(148.3±44.4)pg/ml,TNF-α水平為(22.4±4.7)pg/ml、(261.3±51.4)pg/ml、(235.3±45.9)pg/ml;肺組織IL-6水平為(257.3±55.9)pg/g、(2578.3±403.0)pg/g、(2370.0±491.0)pg/g,IL-10水平為(80.8±20.8)Pg/g、(642.0±107.3)pg/g、(695.3±151.7)Pg/g,TNF-α水平為(207.6±98.6)pg/g、(1769.1±635.6)Pg/g、(1401.1±450.5)pg/g;胰腺病理評分為0、7.0±1.3、6.3±1.0;肺髒病理評分為0、6.3±1.4、5.6±1.0;肺組織TLR4錶達量為0.09 ±0.03、0.59±0.09、0.52±0.08.ANP組和治療組的上述指標均顯著高于假手術組(P值均＜0.01);除IL-10外,治療組的指標又顯著低于ANP組(P值均＜0.05).肺組織病理學損傷與胰腺組織損傷呈正相關(r=0.807,P＜0.01),與TLR4錶達水平亦呈正相關(r=0.519,P＜0.01).結論 大黃甘草湯可快速改善ANP併髮的肺損傷,其機製可能與抑製TLR4的錶達、降低IL-6和TNF-α、升高IL-10水平有關.
목적 관찰대황감초탕대급성배사성이선염(ANP)대서병발폐손상적치료효과,탐토기작용궤제.방법 90지Wistar대서안완전수궤법분위가수술조、ANP조화대황감초탕치료(치료)조,매조30지.채용역행이담관주사4%우광담산납방법제비ANP모형.치료조재제모후급여대황감초탕(0.25 g/ml)0.6 ml/100 g체중관위,1차/12 h;가수술조여ANP조여등량생리염수관위.술후6、12、24 h분비처사대서,취이선급폐조직행병이학검사병평분;측혈청급폐조직IL-6、IL-10、TNF-α수평;면역조화법검측폐조직Toll양수체4(TLR4)적표체량.결과 제모후12 h,가수술조、ANP조화치료조적혈IL-6수평분별위(14.4±4.0)pg/ml、(171.4±41.3)pg/ml、(156.9±34.7)pg/ml,IL-10수평위(13.7±4.5)pg/ml、(120.5±23.7)pg/ml、(148.3±44.4)pg/ml,TNF-α수평위(22.4±4.7)pg/ml、(261.3±51.4)pg/ml、(235.3±45.9)pg/ml;폐조직IL-6수평위(257.3±55.9)pg/g、(2578.3±403.0)pg/g、(2370.0±491.0)pg/g,IL-10수평위(80.8±20.8)Pg/g、(642.0±107.3)pg/g、(695.3±151.7)Pg/g,TNF-α수평위(207.6±98.6)pg/g、(1769.1±635.6)Pg/g、(1401.1±450.5)pg/g;이선병리평분위0、7.0±1.3、6.3±1.0;폐장병리평분위0、6.3±1.4、5.6±1.0;폐조직TLR4표체량위0.09 ±0.03、0.59±0.09、0.52±0.08.ANP조화치료조적상술지표균현저고우가수술조(P치균＜0.01);제IL-10외,치료조적지표우현저저우ANP조(P치균＜0.05).폐조직병이학손상여이선조직손상정정상관(r=0.807,P＜0.01),여TLR4표체수평역정정상관(r=0.519,P＜0.01).결론 대황감초탕가쾌속개선ANP병발적폐손상,기궤제가능여억제TLR4적표체、강저IL-6화TNF-α、승고IL-10수평유관.
Objective To investigate the treatment effects of dahuang gancao decoction on lung Injury complicated by acute necrotizing pancreatitis and explore its mechanism. Methods 90 Wistar rats were randomly divided into 3 groups, namely sham-operated group (SO), ANP group (ANP), dahuang gancao decoction treatment group (DG). 4% sodium taurocholate was injected into the pancreatic duct to induce ANP. Dahuang gancao decoction(0.25 g/ml) 0.6 ml/100 g weight was gavaged in the DG group, the same volume of normal saline was gavaged in the SO and ANP group, which was repeated 12 h later. After 6 h, 12 h, 24 h, all rats were sacrificed, pancreas and lung tissues were harvested to observe the pathological changes and the pathological changes were scored. IL-6, IL-10, TNF-α levels of serum and lung tissue were measured,and the changes of toll-like receptor 4 (TLR4) expression in lung tissue was determined by immunohistochemical method. Results 12 h after ANP induction, the serum levels of IL-6 in SO, ANP, DG group were (14.4±4.0)pg/ml, (171.4 ±41.3)pg/ml, (156.9 ±34.7)pg/ml; the serum levels of IL-10 were (13.7 ±4.5)pg/ml, (120.5 ±23.7)pg/ml, (148.3 ±44.4)pg/ml; the serum levels of TNF-α were (22.4 ±4.7)pg/ml, (261.3 ±51.4)pg/ml, (235.3 ±45.9)pg/ml; the lung tissue levels of IL-6 were (257.3 ±55.9)pg/ml,(2578.3 ±403.0)pg/ml,(2370.0 ±491.0)pg/ml; the lung tissue levels of IL-10 were (80.8 ±20. 8)pg/g, (642.0 ± 107.3)pg/g, (695.3 ± 151.7) pg/g, the lung tissue levels of TNF-α were (207.6 ±98.6)pg/g, (1769.1 ±635.6) pg/g, (1401.1 ±450.5) pg/g; the pancreas pathological scores were 0, 7.00 ±1.33, 6.30 ± 0.95; the lung pathological scores were 0, 6.30 ± 1.42, 5.60 ± 0.97; the expressions of TLR4in lung tissue were 0.09 ± 0. 03, 0.59 ± 0. 09, 0. 52 ± 0. 08. The values in the ANP and DG groups were significantly higher than those in SO group (P < 0. 01 ); except for IL-10, all values in the DG groups were significantly lower than those in ANP group (P < 0.05); there was a positive association between lung and pancreas scores (r =0.807, P<0.01), and lung score was positively associated with the expression of TLR4(r = 0.519, P < 0.01 ). Conclusions Dahuang ganeao decoction may quickly improve lung injury complivated by ANP. The mechanism may involve inhibiting the expression of TLR4 and down-regulating the expression of IL-6, TNF-α, up-regulating the. Expression of IL-10.
