中华小儿外科杂志
中華小兒外科雜誌
중화소인외과잡지
CHINESE JOURNAL OF PEDIATRIC SURGERY
2009年
11期
789-792
,共4页
朱英坚%蒋君涛%马隆%张捷%洪艳%廖凯%刘国华
硃英堅%蔣君濤%馬隆%張捷%洪豔%廖凱%劉國華
주영견%장군도%마륭%장첩%홍염%료개%류국화
邻苯二甲酸二丁酯%尿道下裂%转化生长因子βr%转化生长因子βr Ⅲ
鄰苯二甲痠二丁酯%尿道下裂%轉化生長因子βr%轉化生長因子βr Ⅲ
린분이갑산이정지%뇨도하렬%전화생장인자βr%전화생장인자βr Ⅲ
Di-n-butyl phthalate%Hypospadias%TGF-β1%TGF-βrⅢ
目的 通过验证大鼠孕期污染邻苯二甲酸二丁酯(DBP)所致尿道下裂雄性仔鼠生殖结节中TGF-β1、TGF-βr Ⅲ的表达异常,探讨其在DBP生殖毒性过程中的作用机制.方法 孕鼠20只,随机分二组,在怀孕(GD)14～18 d期间,每天分别灌胃给予大豆油、DBP750 mg/kg,出生第一天(PND1),统计仔鼠出生数,尿道下裂发生率.PND7,雄性仔鼠称重,测量雄性仔鼠肛门至生殖器距离(AGD),拍摄尿道下裂大体图片,取尿道下裂生殖结节进行病理学切片检查,用实时定量逆转录聚合酶链反应(Real-time quantitative PCR)方法 检测生殖结节中的TGF-β1、TGF-βr Ⅲ的mRNA表达水平.结果DBP染毒后导致新生仔鼠与正常组相比数量明显减少,雄性仔鼠尿道下裂的发生率为:43.75%.PND7时,雄性仔鼠体重明显下降,AGD明显缩短.大体图片和病理切片显示典型尿道下裂改变.同时与对照组相比,DBP导致尿道下裂雄性仔鼠生殖结节中TGF-β1、TGF-βr Ⅲ的tuRNA表达水平明显下降.结论 DBP对母体有着明显的毒性作用,DBP干预了TGF-β1、TGF-βr Ⅲ基因的表达,导致子代的生殖结节发育异常,引起尿道下裂.
目的 通過驗證大鼠孕期汙染鄰苯二甲痠二丁酯(DBP)所緻尿道下裂雄性仔鼠生殖結節中TGF-β1、TGF-βr Ⅲ的錶達異常,探討其在DBP生殖毒性過程中的作用機製.方法 孕鼠20隻,隨機分二組,在懷孕(GD)14～18 d期間,每天分彆灌胃給予大豆油、DBP750 mg/kg,齣生第一天(PND1),統計仔鼠齣生數,尿道下裂髮生率.PND7,雄性仔鼠稱重,測量雄性仔鼠肛門至生殖器距離(AGD),拍攝尿道下裂大體圖片,取尿道下裂生殖結節進行病理學切片檢查,用實時定量逆轉錄聚閤酶鏈反應(Real-time quantitative PCR)方法 檢測生殖結節中的TGF-β1、TGF-βr Ⅲ的mRNA錶達水平.結果DBP染毒後導緻新生仔鼠與正常組相比數量明顯減少,雄性仔鼠尿道下裂的髮生率為:43.75%.PND7時,雄性仔鼠體重明顯下降,AGD明顯縮短.大體圖片和病理切片顯示典型尿道下裂改變.同時與對照組相比,DBP導緻尿道下裂雄性仔鼠生殖結節中TGF-β1、TGF-βr Ⅲ的tuRNA錶達水平明顯下降.結論 DBP對母體有著明顯的毒性作用,DBP榦預瞭TGF-β1、TGF-βr Ⅲ基因的錶達,導緻子代的生殖結節髮育異常,引起尿道下裂.
목적 통과험증대서잉기오염린분이갑산이정지(DBP)소치뇨도하렬웅성자서생식결절중TGF-β1、TGF-βr Ⅲ적표체이상,탐토기재DBP생식독성과정중적작용궤제.방법 잉서20지,수궤분이조,재부잉(GD)14～18 d기간,매천분별관위급여대두유、DBP750 mg/kg,출생제일천(PND1),통계자서출생수,뇨도하렬발생솔.PND7,웅성자서칭중,측량웅성자서항문지생식기거리(AGD),박섭뇨도하렬대체도편,취뇨도하렬생식결절진행병이학절편검사,용실시정량역전록취합매련반응(Real-time quantitative PCR)방법 검측생식결절중적TGF-β1、TGF-βr Ⅲ적mRNA표체수평.결과DBP염독후도치신생자서여정상조상비수량명현감소,웅성자서뇨도하렬적발생솔위:43.75%.PND7시,웅성자서체중명현하강,AGD명현축단.대체도편화병리절편현시전형뇨도하렬개변.동시여대조조상비,DBP도치뇨도하렬웅성자서생식결절중TGF-β1、TGF-βr Ⅲ적tuRNA표체수평명현하강.결론 DBP대모체유착명현적독성작용,DBP간예료TGF-β1、TGF-βr Ⅲ기인적표체,도치자대적생식결절발육이상,인기뇨도하렬.
Objective To evaluate the expression of TGF-β1 and TGF-βrⅢ in genital tubercle (GT) of hypospadiac male rats induced by maternal exposure to DBP. Methods Twenty timed preg-nant rats were randomly divided into 2 groups and were given Di-n-butylphthalate (DBP) by gastric in-tubation at a dose of 0 or 750 mg/kg from gestation day(GD) 14 to GD18. On postnatal day (PND 1), live pups were counted and male pups were examined for hypospadias. On PNDT, The anogenital dis-tance (AGD) and body weight of male pups were measured. The gross image and the histologic chan-ges of hypospadiac genitalia were checked. Meanwhile, real-time quantitative PCR were used to quanti-fy mRNA expression of TGF-β1 and TGF-β rⅢ in GT of hypospadiac male rats. Results The number of live pups in the DBP treating group was significantly decreased and the incidence of hypospadias was 43. 750%. The body weight and the AGD of hypospadiac male rats were significantly decreased on PND7. The general and the pathological changes of hypospadiac genitalia were also observed. Moreo-ver,significantly decreased gene expressions of TGF-β1 and TGF-βr Ⅲ were found. Conclusions There were obviously toxic effects of DBP to the pregnant rats. The down-regulation expression of TGF-β signaling molecules might lead to the development of hypospadias induced by maternal exposure to DBP.
