中国医药
中國醫藥
중국의약
CHINA MEDICINE
2010年
11期
1060-1062
,共3页
紫癜,过敏性%免疫共刺激分子%单核细胞
紫癜,過敏性%免疫共刺激分子%單覈細胞
자전,과민성%면역공자격분자%단핵세포
Purpura,Henoch-Schonlein%4-1BB%Monocytes
目的 探讨免疫共刺激分子4-1BB/4-1BBL在过敏性紫癜(HSP)患儿外周血单个核细胞(PBMC)中的表达及其意义. 方法 34例仞发HSP患儿以临床表现有无明显肾脏损害分为紫癜性肾炎(HSPN)组13例,过敏性紫癜无肾脏受累(NO-HSPN)组21例,同期体检健康儿童20例为正常对照组.应用流式细胞仪(FCM)检测3组入选者PBMC中4-1BB和4-1BBL的蛋白表达水平. 结果 HSP患儿4-1BB[(1.32±0.51)%、(1.36±0.62)%]、4-1BBL[(6.07±1.67)%、(7.14±1.94)%]蛋白的表达明显高于正常对照组[(0.70±0.25)%、(3.64±1.28)%],差异均有统计学意义(P＜0.01).加入植物血凝素(PHA)刺激后,3组4-1BB、4-1BBL蛋白水平的表达均明显增高[(46.54±8.19)%比(1.32±0.51)%,(50.31±5.17)%比(1.36±0.62)%;(16.31±3.80)%比(6.07±1.67)%,(17.79±2.73)%比(7.14±1.94)%;均P＜0.01],且NO-HSPN及HSPN组较正常对照组升高更明显.加入地塞米松后,HSP患儿4-1BB、4-1BBL蛋白水平的表达均明显减少[(0.62±0.29)%比(1.32±0.51)%,(0.68±0.33)%比(1.36±0.62)%;(3.06±1.33)%比(6.07±1.67)%,(3.71±1.27)%比(7.14±1.94)%;均P＜0.01],正常对照组的表达减少不明显. 结论 HSP患儿PBMC共刺激分子4-1BB、4-1BBL蛋白水平的表达异常增高,说明4-1BB、4-1BBI这一对共刺激分子可能参与HSP的起病,提示两者可以作为监测HSP患儿病情以及检测早期肾脏损害的免疫学指标.
目的 探討免疫共刺激分子4-1BB/4-1BBL在過敏性紫癜(HSP)患兒外週血單箇覈細胞(PBMC)中的錶達及其意義. 方法 34例仞髮HSP患兒以臨床錶現有無明顯腎髒損害分為紫癜性腎炎(HSPN)組13例,過敏性紫癜無腎髒受纍(NO-HSPN)組21例,同期體檢健康兒童20例為正常對照組.應用流式細胞儀(FCM)檢測3組入選者PBMC中4-1BB和4-1BBL的蛋白錶達水平. 結果 HSP患兒4-1BB[(1.32±0.51)%、(1.36±0.62)%]、4-1BBL[(6.07±1.67)%、(7.14±1.94)%]蛋白的錶達明顯高于正常對照組[(0.70±0.25)%、(3.64±1.28)%],差異均有統計學意義(P＜0.01).加入植物血凝素(PHA)刺激後,3組4-1BB、4-1BBL蛋白水平的錶達均明顯增高[(46.54±8.19)%比(1.32±0.51)%,(50.31±5.17)%比(1.36±0.62)%;(16.31±3.80)%比(6.07±1.67)%,(17.79±2.73)%比(7.14±1.94)%;均P＜0.01],且NO-HSPN及HSPN組較正常對照組升高更明顯.加入地塞米鬆後,HSP患兒4-1BB、4-1BBL蛋白水平的錶達均明顯減少[(0.62±0.29)%比(1.32±0.51)%,(0.68±0.33)%比(1.36±0.62)%;(3.06±1.33)%比(6.07±1.67)%,(3.71±1.27)%比(7.14±1.94)%;均P＜0.01],正常對照組的錶達減少不明顯. 結論 HSP患兒PBMC共刺激分子4-1BB、4-1BBL蛋白水平的錶達異常增高,說明4-1BB、4-1BBI這一對共刺激分子可能參與HSP的起病,提示兩者可以作為鑑測HSP患兒病情以及檢測早期腎髒損害的免疫學指標.
목적 탐토면역공자격분자4-1BB/4-1BBL재과민성자전(HSP)환인외주혈단개핵세포(PBMC)중적표체급기의의. 방법 34례인발HSP환인이림상표현유무명현신장손해분위자전성신염(HSPN)조13례,과민성자전무신장수루(NO-HSPN)조21례,동기체검건강인동20례위정상대조조.응용류식세포의(FCM)검측3조입선자PBMC중4-1BB화4-1BBL적단백표체수평. 결과 HSP환인4-1BB[(1.32±0.51)%、(1.36±0.62)%]、4-1BBL[(6.07±1.67)%、(7.14±1.94)%]단백적표체명현고우정상대조조[(0.70±0.25)%、(3.64±1.28)%],차이균유통계학의의(P＜0.01).가입식물혈응소(PHA)자격후,3조4-1BB、4-1BBL단백수평적표체균명현증고[(46.54±8.19)%비(1.32±0.51)%,(50.31±5.17)%비(1.36±0.62)%;(16.31±3.80)%비(6.07±1.67)%,(17.79±2.73)%비(7.14±1.94)%;균P＜0.01],차NO-HSPN급HSPN조교정상대조조승고경명현.가입지새미송후,HSP환인4-1BB、4-1BBL단백수평적표체균명현감소[(0.62±0.29)%비(1.32±0.51)%,(0.68±0.33)%비(1.36±0.62)%;(3.06±1.33)%비(6.07±1.67)%,(3.71±1.27)%비(7.14±1.94)%;균P＜0.01],정상대조조적표체감소불명현. 결론 HSP환인PBMC공자격분자4-1BB、4-1BBL단백수평적표체이상증고,설명4-1BB、4-1BBI저일대공자격분자가능삼여HSP적기병,제시량자가이작위감측HSP환인병정이급검측조기신장손해적면역학지표.
Objective To investigate the expression of costimulatory molecules 4-1BB and 4-1BBL in patients with Henoch-Schonlein purpura(HSP)and explore the possible significance. Methods Thiroy-four HSP patients as research objects were devided into two groups:the first group is 13 Henoch-Schonlein purpura nephritis (HSPN)patients,the second is 21 NO-HSPN patients and the concrol is 20 healthy children.Applying flow cytometry(FCM)technique and phytohaemagglutinin(PHA),dexamethasone(DEX)as intervention to quantitatively analyze 4-1BB and 4-1BBL expression in PBMC(peripheral blood mononuclear cells)of 34 HSP patients. Results Compaired with normal controls,the expression of 4-1BB,4-1BBL positive expression cell rate(PECR)increase obviously in HSP especially in HSPN.The expression of PECR in all objections both increase obviously especially in HSP after add in PHA(P ＜0.01).The expression of 4-1BB,4-1BBL PECR of the control in PBMC decrease little but decrease obviously in NO-HSPN and HSPN after add in Dex(P ＜ 0.01). Conclusions The abnormal expression of 4-1BB and 4-1BBL may play a critical role in the pathogenesis and development of HSP.4-1BB and 4-1BBL may be immunological marker of evaluating the condition of patients with HSP.
