中华生物医学工程杂志
中華生物醫學工程雜誌
중화생물의학공정잡지
CHINESE JOURNAL OF BIOMEDICAL ENGINEERING
2008年
6期
449-452
,共4页
陈雁君%金永久%武文华%张建萍%张雪梅%闫慧芳
陳雁君%金永久%武文華%張建萍%張雪梅%閆慧芳
진안군%금영구%무문화%장건평%장설매%염혜방
灌流%有机磷化合物%阿托品%包膜活性炭%HA230树脂%色谱法,高压液相
灌流%有機燐化閤物%阿託品%包膜活性炭%HA230樹脂%色譜法,高壓液相
관류%유궤린화합물%아탁품%포막활성탄%HA230수지%색보법,고압액상
Perfusion%Organophosphorus compounds%Atropine%Membrane coated activated charcoal%HA230 absorption resin%Chromatography,high pressure liquid
目的 定量研究血液灌流对有机磷农药硫线磷和其解毒药阿托品的吸附作用.方法 模拟临床血液灌流装置,对含硫线磷和硫酸阿托品的血样进行灌流吸附,分别用毛细管气相色谱法和高效液相色谱法测定硫线磷和硫酸阿托品的残留量.结果 吸附剂用量为0.5、1.0和1.5 g,包膜活性炭在灌流2.0 h后硫线磷的清除率均能达到90%以上,硫酸阿托品的清除率依次为61.9%、84.9%和88.9%;HA230树脂在灌流1.5 h后硫线磷清除率都达到90%以上,硫酸阿托品的清除率也依次高达88.0%、97.2%和98.4%;包膜活性炭灌流3.0h后,硫酸阿托品与硫线磷的比值最高为灌流前的10.1倍,而HA230树脂灌流后,此比值最高为灌流前的6.7倍.结论 包膜活性炭和HA230吸附树脂血液灌流1.5～2.0 h均能清除血中大部分硫线磷,而且均能增加血中硫酸阿托品和硫线磷浓度的比值.
目的 定量研究血液灌流對有機燐農藥硫線燐和其解毒藥阿託品的吸附作用.方法 模擬臨床血液灌流裝置,對含硫線燐和硫痠阿託品的血樣進行灌流吸附,分彆用毛細管氣相色譜法和高效液相色譜法測定硫線燐和硫痠阿託品的殘留量.結果 吸附劑用量為0.5、1.0和1.5 g,包膜活性炭在灌流2.0 h後硫線燐的清除率均能達到90%以上,硫痠阿託品的清除率依次為61.9%、84.9%和88.9%;HA230樹脂在灌流1.5 h後硫線燐清除率都達到90%以上,硫痠阿託品的清除率也依次高達88.0%、97.2%和98.4%;包膜活性炭灌流3.0h後,硫痠阿託品與硫線燐的比值最高為灌流前的10.1倍,而HA230樹脂灌流後,此比值最高為灌流前的6.7倍.結論 包膜活性炭和HA230吸附樹脂血液灌流1.5～2.0 h均能清除血中大部分硫線燐,而且均能增加血中硫痠阿託品和硫線燐濃度的比值.
목적 정량연구혈액관류대유궤린농약류선린화기해독약아탁품적흡부작용.방법 모의림상혈액관류장치,대함류선린화류산아탁품적혈양진행관류흡부,분별용모세관기상색보법화고효액상색보법측정류선린화류산아탁품적잔류량.결과 흡부제용량위0.5、1.0화1.5 g,포막활성탄재관류2.0 h후류선린적청제솔균능체도90%이상,류산아탁품적청제솔의차위61.9%、84.9%화88.9%;HA230수지재관류1.5 h후류선린청제솔도체도90%이상,류산아탁품적청제솔야의차고체88.0%、97.2%화98.4%;포막활성탄관류3.0h후,류산아탁품여류선린적비치최고위관류전적10.1배,이HA230수지관류후,차비치최고위관류전적6.7배.결론 포막활성탄화HA230흡부수지혈액관류1.5～2.0 h균능청제혈중대부분류선린,이차균능증가혈중류산아탁품화류선린농도적비치.
Objective To study on adsorption effect of cadusafos and atropine sulfate by hemoperfusion.Method Hemoperfusions were performed for sheep blood samples with cadusafos and atropineby through imitated extracorporeal closed circulating perfusion apparatus.Residual cadusafos was determined by gas chromatography and residual atropine was determined by high performance liquid chromatography.Result Dose of adsorption agent was 0.5,1.0 and 1.5 g,respectively.Two hours after hemoperfusion with membrane coated activated charcoal,clearance rate of cadusafos in 3 groups all exceeded 90%.and clearance rate of atropine sulfate was 61.9%,84.9%,88.9%,respectively.One and a half hours after hemoperfusion with HA230 absorption resin,clearance rate of eadusafos in 3 groups all exceeded 90%,and clearance rate of atropine sulfate was 88.0%,97.2%,98.4%,respectively.Three hours after hemoperfusion with membrane coated activated charcoal,The concentration ratio of cadusafos and atropine sulfate in blood promoted to 10.1 times,and the ratio was 6.7 times after hemoperfusion with HA230 absorption resin.Conclusion It suggested that cadusafos were mostly removed from blood after 1.5～2.0hours hemoperfusion with membrane activated charcoal or HA230 absorption resin.The concentration ratio of cadusafos and atropine sulfate in blood will increased after hemoperfusion.
