CAJ | 학술논문

目的探讨胰腺癌的细胞遗传学特征.方法采用光谱核型分析技术对中国人胰腺癌细胞系P2的染色体核型进行分析,并选择EGFR/CEP 7双色荧光原位杂交(FISH)探针,对比分析10例胰腺癌和10例慢性胰腺炎石蜡标本的EGFR基因拷贝数,验证光谱核型分析结果.结果 P2细胞系为亚三倍体核型,共发现26种染色体异常,其中重复出现的染色体异常改变为染色体4、9、18、19、22、Y、10p、15p、8p、6q和12p缺失,染色体7和12q增加,以及染色体结构畸变der(9;15)(q10;q10)、der(10)(3;10)(?;q26)和der(12)(8;12)(?;p13).EGFR-FISH阳性为4/10.结论胰腺癌细胞系的染色体重排非常复杂,进一步扩大样本量进行相关分析,包括了解胰腺癌的EGFR-FISH阳性率非常有必要.
목적 탐토이선암적세포유전학특정.방법 채용광보핵형분석기술대중국인이선암세포계P2적염색체핵형진행분석,병선택EGFR/CEP 7쌍색형광원위잡교(FISH)탐침,대비분석10례이선암화10례만성이선염석사표본적EGFR기인고패수,험증광보핵형분석결과.결과 P2세포계위아삼배체핵형,공발현26충염색체이상,기중중복출현적염색체이상개변위염색체4、9、18、19、22、Y、10p、15p、8p、6q화12p결실,염색체7화12q증가,이급염색체결구기변der(9;15)(q10;q10)、der(10)(3;10)(?;q26)화der(12)(8;12)(?;p13).EGFR-FISH양성위4/10.결론 이선암세포계적염색체중배비상복잡,진일보확대양본량진행상관분석,포괄료해이선암적EGFR-FISH양성솔비상유필요.
Objective To investigate the chromosomal characteristics of pancreatic ductal adenocarcinomas by spectral karyotyping. Methods Cytogenetic aberrations of pancreatic cancer cell line P2 established from a Chinese patient was investigated by spectral karyotyping (SKY). Chromosomal alterations were further evaluated in 10 cases of pancreatic cancer and 10 cases of chronic pancreatitis by two color fluorescence in situ hybridization (FISH) by using EGFR/CEP7 probe and paraffin embedded tissue samples. Results Hypotriploid and 26 chromosomal aberrations were revealed in cell line P2. Recurrent chromosomal numerical alterations included loss of chromosome 4,9, 18,19,22, Y, 10p, 15p, 8p, 6q and 12p, with gain of chromosome 7 and 12q. Frequent chromosomal structural abnormalities included der (9;15) (q10;q10), der(10) (3;10) (?;q26) and der(12) (8;12) (?;p13). Four of 10 cases showed EGFR copy number changes by FISH. Conclusions Highly complex chromosomal rearrangements occur in pancreatic cancers. Additional studies of more cases are needed, including FISH analysis of EGFR copy number changes, to reach a conclusion.