中华普通外科杂志
中華普通外科雜誌
중화보통외과잡지
CHINESE JOURNAL OF GENERAL SURGERY
2009年
5期
390-393
,共4页
余强锋%周剑寅%尹震宇%赵文秀%王效民
餘彊鋒%週劍寅%尹震宇%趙文秀%王效民
여강봉%주검인%윤진우%조문수%왕효민
肝功能衰竭,急性%干细胞移植%胚胎干细胞%动物实验
肝功能衰竭,急性%榦細胞移植%胚胎榦細胞%動物實驗
간공능쇠갈,급성%간세포이식%배태간세포%동물실험
Liver failure,acute%Stem cell transplantation%Embryonic stem celia%Animal experimentation
目的 探讨胚胎肝脏原始/干细胞移植治疗急性肝功能损伤裸鼠的可能性与有效性.方法 酶消化法分离孕13.5 d的BALB/C小鼠的胚胎肝脏原始/干细胞,免疫荧光染色法及RT-PCR法鉴定AFP、Albumin、CK19、c-Met等肝系和胆系标志物,给四氯化碳(CCl4)诱发的肝毒性损伤的裸鼠尾静脉注射4×105个干细胞,于注射前和注射后第1、2、4、7天取裸鼠血清,测定肝功能指标,观察肝脏组织学的改善情况.结果 经分离可得到具有干细胞特点的胚肝原始细胞,治疗组裸鼠在干细胞移植后肝功能指标逐渐降低,第1、2、4天均低于对照组,治疗组裸鼠肝脏组织学的病理损伤的恢复比对照组快. 结论酶消化法能够分离得到较多胚胎肝脏原始/干细胞,并且具有一定的改善急性肝损伤裸鼠的肝功能及肝脏病理指标的作用.
目的 探討胚胎肝髒原始/榦細胞移植治療急性肝功能損傷裸鼠的可能性與有效性.方法 酶消化法分離孕13.5 d的BALB/C小鼠的胚胎肝髒原始/榦細胞,免疫熒光染色法及RT-PCR法鑒定AFP、Albumin、CK19、c-Met等肝繫和膽繫標誌物,給四氯化碳(CCl4)誘髮的肝毒性損傷的裸鼠尾靜脈註射4×105箇榦細胞,于註射前和註射後第1、2、4、7天取裸鼠血清,測定肝功能指標,觀察肝髒組織學的改善情況.結果 經分離可得到具有榦細胞特點的胚肝原始細胞,治療組裸鼠在榦細胞移植後肝功能指標逐漸降低,第1、2、4天均低于對照組,治療組裸鼠肝髒組織學的病理損傷的恢複比對照組快. 結論酶消化法能夠分離得到較多胚胎肝髒原始/榦細胞,併且具有一定的改善急性肝損傷裸鼠的肝功能及肝髒病理指標的作用.
목적 탐토배태간장원시/간세포이식치료급성간공능손상라서적가능성여유효성.방법 매소화법분리잉13.5 d적BALB/C소서적배태간장원시/간세포,면역형광염색법급RT-PCR법감정AFP、Albumin、CK19、c-Met등간계화담계표지물,급사록화탄(CCl4)유발적간독성손상적라서미정맥주사4×105개간세포,우주사전화주사후제1、2、4、7천취라서혈청,측정간공능지표,관찰간장조직학적개선정황.결과 경분리가득도구유간세포특점적배간원시세포,치료조라서재간세포이식후간공능지표축점강저,제1、2、4천균저우대조조,치료조라서간장조직학적병리손상적회복비대조조쾌. 결론매소화법능구분리득도교다배태간장원시/간세포,병차구유일정적개선급성간손상라서적간공능급간장병리지표적작용.
Objective To isolate and indentify fetal hepatic progenitor/stem cells, and study the feasibility and effectiveness of their transplantation on acute liver injury in nude mice. Methods The primitive cells isolated from 13.5dpc pregnant mouse fetal hver by way of enzyme digesting were cultured in vitro and liver specific markers as AFP, CK19, Albumin, c-Met, were identified by immunofluorescence and RT-PCR on colonies. 4 × 105 cells were transplanted into nude mice with carbon tetrachloride induced acute liver injury. Hapatic functions were measured pre- and -post transplantation on days 1,2,4, 7. Meanwhile, hepatic pathology was studied. Results Compared to control group the hepatic functions gradually recovered in transplant group, on days 1,2,4 after fetal hepatic stem cell transplantation. The hepatic pathology significantly improved in stem cells transplantation group. Conclution Fetal hepatic progenitor/stem cell were successfully yielded by enzyme digest. Stem cells transplantation improved the hepatic function and pathology in acute hapatic injury model of nude mice.