中国医师进修杂志
中國醫師進脩雜誌
중국의사진수잡지
CHINESE JOURNAL OF POSTGRADUATES OF MEDICINE
2012年
12期
4-6
,共3页
基因,抑制%乳头状瘤病毒感染%宫颈肿瘤%宫颈上皮内瘤样病变
基因,抑製%乳頭狀瘤病毒感染%宮頸腫瘤%宮頸上皮內瘤樣病變
기인,억제%유두상류병독감염%궁경종류%궁경상피내류양병변
Genes,suppressor%Papillomavirus infections%Uterine cervical neoplasms%Cervical intraepithelial neoplasia
目的 研究宫颈癌及癌前病变中凋亡抑制基因Survivin的表达及高危型-人乳头瘤病毒(HR-HPV)的感染情况,探讨两者在宫颈癌发病机制中的作用.方法 入选宫颈上皮内瘤变(CIN)Ⅰ 25例、CINⅡ～Ⅲ23例及宫颈癌34例,另取20例正常宫颈作为对照.采用免疫组织化学SP法检测Survivin的表达,以聚合酶链反应技术检测HR-HPV感染情况.结果 Survivin阳性表达率和HR-HPV感染率均为宫颈癌[85.3％(29/34)和88.2％ (30/34)]高于CIN [52.1％(25/48)和54.2％(26/48) ](P＜ 0.05),CIN高于正常宫颈[0和10.0％(2/20)](P＜0.05),CINⅡ～Ⅲ[65.2％(15/23)和73.9％(17/23)]高于CIN I[32.0％(8/25)和28.0％(7/25)](P＜ 0.05).在宫颈癌中Survivin阳性表达与组织学分级相关(P＜0.05),而与年龄、临床分期及病理分型无关(P＞0.05).HR-HPV感染与年龄、临床分期、组织学分级及病理分型均无关(P＞0.05).在宫颈癌中Survivin阳性表达与HR-HPV感染呈正相关(r=0.403,P＜ 0.05).结论 Survivin阳性表达及HR-HPV感染可能与宫颈癌的发生、发展密切相关.两者在宫颈癌的发病机制中可能起着协同作用.
目的 研究宮頸癌及癌前病變中凋亡抑製基因Survivin的錶達及高危型-人乳頭瘤病毒(HR-HPV)的感染情況,探討兩者在宮頸癌髮病機製中的作用.方法 入選宮頸上皮內瘤變(CIN)Ⅰ 25例、CINⅡ～Ⅲ23例及宮頸癌34例,另取20例正常宮頸作為對照.採用免疫組織化學SP法檢測Survivin的錶達,以聚閤酶鏈反應技術檢測HR-HPV感染情況.結果 Survivin暘性錶達率和HR-HPV感染率均為宮頸癌[85.3％(29/34)和88.2％ (30/34)]高于CIN [52.1％(25/48)和54.2％(26/48) ](P＜ 0.05),CIN高于正常宮頸[0和10.0％(2/20)](P＜0.05),CINⅡ～Ⅲ[65.2％(15/23)和73.9％(17/23)]高于CIN I[32.0％(8/25)和28.0％(7/25)](P＜ 0.05).在宮頸癌中Survivin暘性錶達與組織學分級相關(P＜0.05),而與年齡、臨床分期及病理分型無關(P＞0.05).HR-HPV感染與年齡、臨床分期、組織學分級及病理分型均無關(P＞0.05).在宮頸癌中Survivin暘性錶達與HR-HPV感染呈正相關(r=0.403,P＜ 0.05).結論 Survivin暘性錶達及HR-HPV感染可能與宮頸癌的髮生、髮展密切相關.兩者在宮頸癌的髮病機製中可能起著協同作用.
목적 연구궁경암급암전병변중조망억제기인Survivin적표체급고위형-인유두류병독(HR-HPV)적감염정황,탐토량자재궁경암발병궤제중적작용.방법 입선궁경상피내류변(CIN)Ⅰ 25례、CINⅡ～Ⅲ23례급궁경암34례,령취20례정상궁경작위대조.채용면역조직화학SP법검측Survivin적표체,이취합매련반응기술검측HR-HPV감염정황.결과 Survivin양성표체솔화HR-HPV감염솔균위궁경암[85.3％(29/34)화88.2％ (30/34)]고우CIN [52.1％(25/48)화54.2％(26/48) ](P＜ 0.05),CIN고우정상궁경[0화10.0％(2/20)](P＜0.05),CINⅡ～Ⅲ[65.2％(15/23)화73.9％(17/23)]고우CIN I[32.0％(8/25)화28.0％(7/25)](P＜ 0.05).재궁경암중Survivin양성표체여조직학분급상관(P＜0.05),이여년령、림상분기급병리분형무관(P＞0.05).HR-HPV감염여년령、림상분기、조직학분급급병리분형균무관(P＞0.05).재궁경암중Survivin양성표체여HR-HPV감염정정상관(r=0.403,P＜ 0.05).결론 Survivin양성표체급HR-HPV감염가능여궁경암적발생、발전밀절상관.량자재궁경암적발병궤제중가능기착협동작용.
Objective To investigate the expressions of Survivin and high risk-human papillomavirus (HR-HPV) in cervical carcinoma and premalignant lesion,and explore their roles in the pathogenesis of cervical carcinoma.Methods Eighty-two patients of cervical intraepithelial neoplasia(CIN)and cervical carcinoma were enrolled,including 25 CIN Ⅰ,23 CIN Ⅱ - Ⅲ and 34 cervical carcinoma.Twenty normal cervicals were chosen as control.The expression of Survivin was examined by immunohistochemical assay (SP),and the infection of HR-HPV was measured by polymerase chain reaction (PCR).Results Expressions of Survivin and HR-HPV of patients with cervical carcinoma [ 85.3％(29/34)and 88.2％ (30/34) ]were higher than those in CIN [ 52.1％ (25/48) and 54.2％ (26/48) ](P ＜ 0.05 ),CIN were higher than those in normal cervicals [ 0 and 10.0％ ( 2/20 ) ](P ＜ 0.05 ),CIN Ⅱ - Ⅲ [ 65.2％ ( 15/23 ) and 73.9％ ( 17/23 ) ]were higher than those in CIN Ⅰ [ 32.0％ ( 8/25 ) and 28.0％ (7/25) ](P ＜ 0.05 ).Survivin positive expression in cervical carcinoma was correlation with histological grade,but was no correlation with (P ＜ 0.05),age,clinical stage and pathology typing (P ＞ 0.05 ).HR-HPV infection was no correlation with age,clinical stage,histological grade and pathology typing (P ＞ 0.05).Positive correlation between the Survivin positive expression and HR-HPV infection was observed in cervical carcinoma (r =0.403,P ＜0.05).Conclusion It suggests that Survivin may play an important role in the occurrence and development of cervical carcinoma together with HR-HPV.
