CAJ | 학술논문

目的观察骨髓间充质干细胞(BMSCs)及其分化的神经样细胞与聚乳酸-羟基乙酸(PLGA)支架材料的相容性,并探讨两者复合修复周围神经损伤的效果.方法 (1)在体外,通过荧光显微镜、流式细胞仪、扫描电镜等技术观察BMSCs在PLGA上的生长及分化成神经样细胞的情况；(2)在体内,取右侧坐骨神经损伤的SD大鼠,随即分为4组,分别移植单纯PLGA、BMSCs-PLGA、神经样细胞-PLGA及直接缝合神经,术后斜板试验观察4组动物下肢功能情况；60d后处死大鼠,取神经标本做病理切片.结果 (1)在体外,BMSCs在PLGA上生长良好,与同期培养板里面的细胞比较,吸光度值(A)差异无统计学意义(P＞0.05)；(2)在体内,BMSCs及其神经样细胞复合PLGA后能修复损伤的坐骨神经,促进大鼠下肢运动功能的恢复.术后3周,各组大鼠斜板试验的角度分别是:直接缝合组(38.32 ±5.51)度、单纯PLGA组(40.27 ±2.74)度、BMSCs-PLGA组(45.14±3.43)度、神经样细胞-PLGA组(60.56±4.70)度,且神经样细胞-PLGA组显著高于其他组(P＜0.01)；病理切片显示支架与宿主神经组织有良好的生物相容性.结论 BMSCs能诱导分化生成神经样细胞,而PLGA与两者有良好的生物相容性.
목적 관찰골수간충질간세포(BMSCs)급기분화적신경양세포여취유산-간기을산(PLGA)지가재료적상용성,병탐토량자복합수복주위신경손상적효과.방법 (1)재체외,통과형광현미경、류식세포의、소묘전경등기술관찰BMSCs재PLGA상적생장급분화성신경양세포적정황；(2)재체내,취우측좌골신경손상적SD대서,수즉분위4조,분별이식단순PLGA、BMSCs-PLGA、신경양세포-PLGA급직접봉합신경,술후사판시험관찰4조동물하지공능정황；60d후처사대서,취신경표본주병리절편.결과 (1)재체외,BMSCs재PLGA상생장량호,여동기배양판리면적세포비교,흡광도치(A)차이무통계학의의(P＞0.05)；(2)재체내,BMSCs급기신경양세포복합PLGA후능수복손상적좌골신경,촉진대서하지운동공능적회복.술후3주,각조대서사판시험적각도분별시:직접봉합조(38.32 ±5.51)도、단순PLGA조(40.27 ±2.74)도、BMSCs-PLGA조(45.14±3.43)도、신경양세포-PLGA조(60.56±4.70)도,차신경양세포-PLGA조현저고우기타조(P＜0.01)；병리절편현시지가여숙주신경조직유량호적생물상용성.결론 BMSCs능유도분화생성신경양세포,이PLGA여량자유량호적생물상용성.
Objective To study separately the compatibility between bone marrow mesenchymal stem cells (BMSCs) and polylactic glycolic acid (PLGA) scaffolds,or between differentiated neural-like cells and PLGA,and to explore the effects of both composites repairing peripheral nerve injury.Methods ( 1 ) In vitro,BMSCs were cultured by differential adherence,and composited PLGA,via fluorescence microscope,flow cytometry(FCM) and scanning electron microscope was also applied to investigate the information of BMSCs' adhesion and induction into neural-like cells in the scaffolds.(2) In vivo,SD rat models with the injury for right sciatic nerve,were randomly divided into 4 groups.Bold scaffold,BMSCs-PLGA complex,and neural-like cells-PLGA complex were separately transplanted into SD rat models,and direct never suture.The ramp test was used to obsvered lower extremity motor function of SD rat models after operation.Nerve biopsy specimens was assayed after SD rat were killed in 60 days.Results ( 1 ) In vitro,BMSCs can be exactly grow on the PLGA.It is have no different in optical density value(OD) compared to cells inside the culture plates ( P ＞ O.05 ).②In vivo,BMSCs and nerve-like cells combined with PLGA,which can restore to peripheral nerve injury,and promote the recovery of lower extremity motor function of SD rat models.After operation for 3 weeks,the angle of ramp test in direct suture groups,bold scaffold groups,BMSCs-PLGA groups,and neural-like cells-PLGA groups was (38.32 ± 5.51 )°,(40.27 ± 2.74)°,(45.14 ±3.43 ) °,(60.56 ± 4.70 ) °,and neural-like cells-PLGA groups was significantly higher than other groups.Pathological examination showed nerve tissue with the host stand a good biocompatibility.Conclusion BMSCs can be induction into neural-like cells;PLGA have good compatibility with them.