CAJ | 학술논문

背景:近年来研究结果表明经颅磁刺激对学习记忆能力产生影响,一些研究也开始探讨在模拟脑电节律条件下的磁场对记忆能力的影响.目的:探讨磁场对记忆再现能力的影响及其作用机制.设计:随机对照实验.单位:天津医科大学医学影像学系和天津大学精密仪器及光电子工程学院.材料:实验于2002-02/2004-02在天津大学生物医学工程实验室及天津医科大学电镜中心完成,25只Wistar大鼠和5只家猫由天津医科大学实验动物中心提供.干预:将25只Wister大鼠,随机分为5组,4个实验组和1个对照组,每组5只大鼠.实验1～4组接受调制脉冲磁场刺激,但各组作用时间及调制频率变化规律不同.实验1组接受α节律(10Hz)调制脉冲磁场刺激;实验2组接受β节律(20Hz)调制脉冲磁场刺激;实验3组采用先α节律后β节律波刺激;实验4组采用先β节律后α节律波刺激.记录大鼠记忆再现能力和脑内神经递质的变化.5只家猫先采用先α节律后β节律的脉冲磁场作用20 min,比较磁场作用前后家猫的脑电节律的变化.主要观察指标:①采用改良"十"字迷宫法测试大鼠长时空间记忆再现能力的变化.②用高效液相色谱仪检测磁场作用后大鼠脑海马神经递质.③利用多导生理记录仪记录家猫脑电功率的变化.结果:25只Wistar大鼠和5只家猫均进入结果分析.①大鼠记忆再现能力比较:弱磁场刺激后5 h实验2组、4组明显高于对照组[(11.81±2.80,16.39±3.09,5.77±2.41)s,(P＜0.05,P＜0.01)].②大鼠脑海马多巴胺含量:实验组明显高于对照组[(192.72±18.66,148.17±24.52)ng/g,(P＜0.05)].③家猫β节律变化功率变化:脑电β频率段在功率谱中的面积百分比与刺激前相比显著增加(23.0±3.4,14.2±1.3)%.结论:大多数模拟脑电节律的弱磁场作用明显降低大鼠长时记忆再现能力,但特殊节律的弱磁场可能会提高大鼠记忆再现能力.这些影响与受钙离子内流及突触小泡调控的海马神经递质释放有关. 揹景:近年來研究結果錶明經顱磁刺激對學習記憶能力產生影響,一些研究也開始探討在模擬腦電節律條件下的磁場對記憶能力的影響.目的:探討磁場對記憶再現能力的影響及其作用機製.設計:隨機對照實驗.單位:天津醫科大學醫學影像學繫和天津大學精密儀器及光電子工程學院.材料:實驗于2002-02/2004-02在天津大學生物醫學工程實驗室及天津醫科大學電鏡中心完成,25隻Wistar大鼠和5隻傢貓由天津醫科大學實驗動物中心提供.榦預:將25隻Wister大鼠,隨機分為5組,4箇實驗組和1箇對照組,每組5隻大鼠.實驗1～4組接受調製脈遲磁場刺激,但各組作用時間及調製頻率變化規律不同.實驗1組接受α節律(10Hz)調製脈遲磁場刺激;實驗2組接受β節律(20Hz)調製脈遲磁場刺激;實驗3組採用先α節律後β節律波刺激;實驗4組採用先β節律後α節律波刺激.記錄大鼠記憶再現能力和腦內神經遞質的變化.5隻傢貓先採用先α節律後β節律的脈遲磁場作用20 min,比較磁場作用前後傢貓的腦電節律的變化.主要觀察指標:①採用改良"十"字迷宮法測試大鼠長時空間記憶再現能力的變化.②用高效液相色譜儀檢測磁場作用後大鼠腦海馬神經遞質.③利用多導生理記錄儀記錄傢貓腦電功率的變化.結果:25隻Wistar大鼠和5隻傢貓均進入結果分析.①大鼠記憶再現能力比較:弱磁場刺激後5 h實驗2組、4組明顯高于對照組[(11.81±2.80,16.39±3.09,5.77±2.41)s,(P＜0.05,P＜0.01)].②大鼠腦海馬多巴胺含量:實驗組明顯高于對照組[(192.72±18.66,148.17±24.52)ng/g,(P＜0.05)].③傢貓β節律變化功率變化:腦電β頻率段在功率譜中的麵積百分比與刺激前相比顯著增加(23.0±3.4,14.2±1.3)%.結論:大多數模擬腦電節律的弱磁場作用明顯降低大鼠長時記憶再現能力,但特殊節律的弱磁場可能會提高大鼠記憶再現能力.這些影響與受鈣離子內流及突觸小泡調控的海馬神經遞質釋放有關.
배경:근년래연구결과표명경로자자격대학습기억능력산생영향,일사연구야개시탐토재모의뇌전절률조건하적자장대기억능력적영향.목적:탐토자장대기억재현능력적영향급기작용궤제.설계:수궤대조실험.단위:천진의과대학의학영상학계화천진대학정밀의기급광전자공정학원.재료:실험우2002-02/2004-02재천진대학생물의학공정실험실급천진의과대학전경중심완성,25지Wistar대서화5지가묘유천진의과대학실험동물중심제공.간예:장25지Wister대서,수궤분위5조,4개실험조화1개대조조,매조5지대서.실험1～4조접수조제맥충자장자격,단각조작용시간급조제빈솔변화규률불동.실험1조접수α절률(10Hz)조제맥충자장자격;실험2조접수β절률(20Hz)조제맥충자장자격;실험3조채용선α절률후β절률파자격;실험4조채용선β절률후α절률파자격.기록대서기억재현능력화뇌내신경체질적변화.5지가묘선채용선α절률후β절률적맥충자장작용20 min,비교자장작용전후가묘적뇌전절률적변화.주요관찰지표:①채용개량"십"자미궁법측시대서장시공간기억재현능력적변화.②용고효액상색보의검측자장작용후대서뇌해마신경체질.③이용다도생리기록의기록가묘뇌전공솔적변화.결과:25지Wistar대서화5지가묘균진입결과분석.①대서기억재현능력비교:약자장자격후5 h실험2조、4조명현고우대조조[(11.81±2.80,16.39±3.09,5.77±2.41)s,(P＜0.05,P＜0.01)].②대서뇌해마다파알함량:실험조명현고우대조조[(192.72±18.66,148.17±24.52)ng/g,(P＜0.05)].③가묘β절률변화공솔변화:뇌전β빈솔단재공솔보중적면적백분비여자격전상비현저증가(23.0±3.4,14.2±1.3)%.결론:대다수모의뇌전절률적약자장작용명현강저대서장시기억재현능력,단특수절률적약자장가능회제고대서기억재현능력.저사영향여수개리자내류급돌촉소포조공적해마신경체질석방유관.
BACKGROUND: Recently, many researches suggest that transcranial magnetic stimulation has effect on learning and memory. Moreover, the effects of magnetic fields simulating electroencephalogram (EEG) rhythm on memory have been investigated.OBJECTIVE: To explore the effect of magnetic field simulating EEG rhythm on memory retrieval ability and its mechanism.DESIGN: Randomized controlled study.SETTING: Department of Medical Imaging, Tianjin Medical University,and School of Precision Instrument & Photoelectronic Engineering, Tianjin University.MATERIALS: The experiment was conducted at the Electroscopic Center of TianjSn Medical University and Laboratory of Biomedical Engineering Department of Tianjin University from February 2002 to February 2004.Totally 25 Wistar rats and 5 domestic cats were selected from the Experimental Animal Center of Tianjin Medical University.INTERVENTIONS: All the 25 Wister rats were randomly divided into 5 group, 4 experimental groups and one control group with 5 rats in each group. Magnetic field simulating EEG rhythm was used to stimulate Wistar rats in the 4 experimental groups, but the time and frequency rate were different. Group 1 was stimulated by weak magnetic field with frequency in α rhythm (10 Hz); Group 2 was stimulated by weak magnetic field with frequency in β rhythm (20 Hz); Group 3 was stimulated by weak magnetic field with frequency changing from α to β rhythm; and Group 4 was stimulated by weak magnetic field with frequency changing from β to α rhythm. Memory retrieval ability and changes of neurotransmitters in the brain were recorded. Five cats were treated with weak magnetic field with frequency changing from α to β rhythm for 20 minutes, and the changes of EEG power spectra before and after stimulation were compared.information from long-term memory was tested with modified cross maze;hippocampus of the rats was measured with high performance liquid multiple-channel electrophysiological recording device.Comparison of memory retrieval ability: Memory retrieval ability in Group 2 and Group 4 was obviously higher than that in control group 5 hours after Content ofdopamine (DA) in rats' cerebral hippocampus: It was obviously higher in the 4 experimental groups than in control group [(192.72±18.66,percentage of power spectrum of β rhythm was increased after stimulation [(23.0±3.4, 14.2±1.3) %.].CONCLUSION: Most of the weak magnetic fields simulating EEG rhythm of human brain can decrease the retrieval of long-term memory, but magnetic field with special rhythm may increase memory retrieval ability.All the effects are related to the release of hippocampal neurotransmitters which is mediated by the change of intracellular calcium ([Ca2+]i) and synaptic vesicle.