CAJ | 학술논문

目的探讨连续培养的牙乳头细胞的功能学特征.方法连续培养钟状期猪牙乳头细胞,应用间接法免疫组化检测各代细胞的牙本质涎蛋白(DSP)、核心结合因子1(Cbfa1)、Ⅰ型胶原(ColⅠ)、Ⅲ型胶原(ColⅢ)、骨桥蛋白(OPN)、骨粘蛋白(ON)、骨钙蛋白(OCN)等的表达情况,并以第4代猪骨髓基质细胞及人成纤维细胞作为对照组.结果免疫组化结果可见连续培养的各代猪牙乳头细胞均表达DSP、ColⅠ及ON,所有各代猪牙乳头细胞均未见OCN表达,ColⅢ只在第1代的少量细胞中有表达,Cbfa1及OPN在各代细胞中均有表达.结论体外培养的猪牙乳头细胞具有旺盛的分泌能力,可能具有形成钙化组织的潜能和形成牙本质样结构的可能性.
목적 탐토련속배양적아유두세포적공능학특정.방법 련속배양종상기저아유두세포,응용간접법면역조화검측각대세포적아본질연단백(DSP)、핵심결합인자1(Cbfa1)、Ⅰ형효원(ColⅠ)、Ⅲ형효원(ColⅢ)、골교단백(OPN)、골점단백(ON)、골개단백(OCN)등적표체정황,병이제4대저골수기질세포급인성섬유세포작위대조조.결과 면역조화결과가견련속배양적각대저아유두세포균표체DSP、ColⅠ급ON,소유각대저아유두세포균미견OCN표체,ColⅢ지재제1대적소량세포중유표체,Cbfa1급OPN재각대세포중균유표체.결론 체외배양적저아유두세포구유왕성적분비능력,가능구유형성개화조직적잠능화형성아본질양결구적가능성.
Objective To investigate the characteristics of protein expression of the porcine dental papilla cells (pDPCs) cultured in vitro.Methods pDPCs were continuous cultured by enzyme digestion method. Then immunochemistry method was performed to detect the expression of DSP, Cbfa1, Col Ⅰ, Col Ⅲ, OPN, ON, and OCN.Results The immunohistochemistry results show that DSP, ColⅠ and ON were postively expressed in every generation pDPCs. There was no OCN expression in the cells of every generation. As to ColⅢ, only weak positive expression was detected in the cells of1st generation. Cbfa1 and OPN were positively expression in every generation cells.Conclusion The pDPCs cultured in vitro have strong hearty ability of protein secretion and possibility of forming calcification tissues or dentin-like hard tissues.