中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2010年
5期
309-311
,共3页
薛耀华%郑和平%杨斌%周细玉%吴兴中%黄进梅%曾维英
薛耀華%鄭和平%楊斌%週細玉%吳興中%黃進梅%曾維英
설요화%정화평%양빈%주세옥%오흥중%황진매%증유영
衣原体%沙眼%基因型%微生物敏感性试验
衣原體%沙眼%基因型%微生物敏感性試驗
의원체%사안%기인형%미생물민감성시험
Chlamydia trachomatis%Genotype%Microbial sensitivity tests
目的 探讨广州地区沙眼衣原体临床株对6种抗菌药物的敏感性和基因型分布,以及两者之间的关系.方法 细胞培养法分离衣原体临床株,测定6种抗菌药物对沙眼衣原体的最低抑菌浓度(MIC)和最低杀菌浓度(MBC).巢式PCR扩增沙眼衣原体主要外膜蛋白基因(omp1)的VS1-2片段,核酸测序分型.结果 346例患者的泌尿生殖道标本接种后,获得76株沙眼衣原体临床分离株,其中40株传代后达到药敏试验所需菌量.药敏试验结果显示克拉霉素、阿奇霉素、罗红霉素、多西环素、四环素和氧氟沙星的MIC50/MIC90(μg/ml)分别为0.008/0.032、0.08/0.16、0.125/0.5、0.032/0.064、0.25/0.5、0.5/1.0.40株共检出7种基因型,其中E型有14株(35%),其次为J型10株(25%)和F型6株(15%).7种基因型衣原体菌株对阿奇霉素的MIC50完全相同,而罗红霉素、克拉霉素和氧氟沙星的MIC50在不同型别菌株间有1~4倍的差异.结论 克拉霉素、多西环素和阿奇霉素显示较强的抗衣原体活性,7种基因型衣原体对阿奇霉素显示一致的敏感性.
目的 探討廣州地區沙眼衣原體臨床株對6種抗菌藥物的敏感性和基因型分佈,以及兩者之間的關繫.方法 細胞培養法分離衣原體臨床株,測定6種抗菌藥物對沙眼衣原體的最低抑菌濃度(MIC)和最低殺菌濃度(MBC).巢式PCR擴增沙眼衣原體主要外膜蛋白基因(omp1)的VS1-2片段,覈痠測序分型.結果 346例患者的泌尿生殖道標本接種後,穫得76株沙眼衣原體臨床分離株,其中40株傳代後達到藥敏試驗所需菌量.藥敏試驗結果顯示剋拉黴素、阿奇黴素、囉紅黴素、多西環素、四環素和氧氟沙星的MIC50/MIC90(μg/ml)分彆為0.008/0.032、0.08/0.16、0.125/0.5、0.032/0.064、0.25/0.5、0.5/1.0.40株共檢齣7種基因型,其中E型有14株(35%),其次為J型10株(25%)和F型6株(15%).7種基因型衣原體菌株對阿奇黴素的MIC50完全相同,而囉紅黴素、剋拉黴素和氧氟沙星的MIC50在不同型彆菌株間有1~4倍的差異.結論 剋拉黴素、多西環素和阿奇黴素顯示較彊的抗衣原體活性,7種基因型衣原體對阿奇黴素顯示一緻的敏感性.
목적 탐토엄주지구사안의원체림상주대6충항균약물적민감성화기인형분포,이급량자지간적관계.방법 세포배양법분리의원체림상주,측정6충항균약물대사안의원체적최저억균농도(MIC)화최저살균농도(MBC).소식PCR확증사안의원체주요외막단백기인(omp1)적VS1-2편단,핵산측서분형.결과 346례환자적비뇨생식도표본접충후,획득76주사안의원체림상분리주,기중40주전대후체도약민시험소수균량.약민시험결과현시극랍매소、아기매소、라홍매소、다서배소、사배소화양불사성적MIC50/MIC90(μg/ml)분별위0.008/0.032、0.08/0.16、0.125/0.5、0.032/0.064、0.25/0.5、0.5/1.0.40주공검출7충기인형,기중E형유14주(35%),기차위J형10주(25%)화F형6주(15%).7충기인형의원체균주대아기매소적MIC50완전상동,이라홍매소、극랍매소화양불사성적MIC50재불동형별균주간유1~4배적차이.결론 극랍매소、다서배소화아기매소현시교강적항의원체활성,7충기인형의원체대아기매소현시일치적민감성.
Objective To assess the vitro susceptibility to 6 antimicrobial agents and genotypes of
clinical isolates of Chlamydia trachomatis (Ct) from Guangzhou region. Methods Ct was isolated from clinical
specimens by using McCoy cell culture and subjected to propagation. The minimum inhibitory concentration
(MIC) and minimal bactericidal concentration (MBC) of 6 antimicrobial agents (clarithromycin, roxithromycin,
azithromycin, doxycycline, tetracycline, ofloxacin) against Ct isolates were determined in McCoy cell culture.
Nested PCR was performed to amplify the outer membrane protein 1 (omp1) VS1-2 gene followed by sequencing.
Results Seventy-six Ct strains were isolated from 346 urogenital specimens, and 40 strains met the require
ments for susceptibility testing after serial propagation. The MIC50/MIC90 of clarithromycin, azithromycin, roxi
thromycin, doxycycline, tetracycline and ofloxacin were as follows: 0.008/0.032, 0.080/0.160, 0.125/0.500,
0.032/0.064, 0.250/0.500 and 0.500/1.000 mg/L. Seven genotypes were observed. The most prevalent geno
types in decreasing order were E (14, 35%), J (10, 25%)and F (6, 15%). The MIC50 was consistent for
azithromycin among the 7 genotypes, but varied by 1 - 4 folds for doxycycline, ofloxacin and roxithromycin.
Conclusions Clarithromycin, doxycycline and azithromycin exhibit an excellent activity against Ct, and the
activity of azithromycin is consistent among the 7 genotypes of Ct.