中华放射肿瘤学杂志
中華放射腫瘤學雜誌
중화방사종류학잡지
CHINESE JOURNAL OF RADIATION ONCOLOGY
2009年
3期
238-242
,共5页
惠周光%骆爱萍%毕楠%张烨%雷明芳%麦伟源%TEH Bin-tean%TEH Bin-sing
惠週光%駱愛萍%畢楠%張燁%雷明芳%麥偉源%TEH Bin-tean%TEH Bin-sing
혜주광%락애평%필남%장엽%뢰명방%맥위원%TEH Bin-tean%TEH Bin-sing
细胞系,肾肿瘤%照射%信号传递和转录活化因子%放射增敏剂
細胞繫,腎腫瘤%照射%信號傳遞和轉錄活化因子%放射增敏劑
세포계,신종류%조사%신호전체화전록활화인자%방사증민제
Cell line,kidney neoplasms%Irradiation%Signal transducer and activator of tran-scription%Radiosenstization agent
目的 研究人肾透明细胞癌信号传递和转录活化因子1(STAT1)表达情况及抑制STAT1对该肿瘤细胞放射敏感性的影响.方法 采用免疫组织化学染色技术对比检测34例人肾透明细胞癌标本和12例正常肾组织标本的STAT1表达.用Western blotting法检测离体培养人肾透明细胞癌细胞(CRL-1932)、纤维母细胞(CCL-116)和wilm's瘤细胞(CRL-1441)的STAT1表达.用氟达拉滨和siRNA抑制CRL-1932细胞STAT1表达,并通过成克隆法和台盼蓝染色计数法研究抑制STAT1对CRL-1932细胞放射敏感性的影响.结果 人肾透明细胞癌标本的总STAT1和磷酸化STAT1表达均明显高于正常肾组织.Western blotting法显示CRL-1932细胞STAT1表达比CRL-1441、CCL-116细胞明显增高;药物氟达拉滨能显著抑制CRL-1932细胞磷酸化STAT1的表达,并显著增加CRL-1932细胞的放射敏感性,且放射增敏程度与药物浓度呈正相关.经STAT1 siRNA处理后CRL-1932细胞总STAT1和磷酸化STAT1的表达均被有效抑制,且细胞在照射后的存活分数显著下降.结论 肾透明细胞癌STAT1呈高表达,抑制STAT1对该细胞有放射增敏作用.
目的 研究人腎透明細胞癌信號傳遞和轉錄活化因子1(STAT1)錶達情況及抑製STAT1對該腫瘤細胞放射敏感性的影響.方法 採用免疫組織化學染色技術對比檢測34例人腎透明細胞癌標本和12例正常腎組織標本的STAT1錶達.用Western blotting法檢測離體培養人腎透明細胞癌細胞(CRL-1932)、纖維母細胞(CCL-116)和wilm's瘤細胞(CRL-1441)的STAT1錶達.用氟達拉濱和siRNA抑製CRL-1932細胞STAT1錶達,併通過成剋隆法和檯盼藍染色計數法研究抑製STAT1對CRL-1932細胞放射敏感性的影響.結果 人腎透明細胞癌標本的總STAT1和燐痠化STAT1錶達均明顯高于正常腎組織.Western blotting法顯示CRL-1932細胞STAT1錶達比CRL-1441、CCL-116細胞明顯增高;藥物氟達拉濱能顯著抑製CRL-1932細胞燐痠化STAT1的錶達,併顯著增加CRL-1932細胞的放射敏感性,且放射增敏程度與藥物濃度呈正相關.經STAT1 siRNA處理後CRL-1932細胞總STAT1和燐痠化STAT1的錶達均被有效抑製,且細胞在照射後的存活分數顯著下降.結論 腎透明細胞癌STAT1呈高錶達,抑製STAT1對該細胞有放射增敏作用.
목적 연구인신투명세포암신호전체화전록활화인자1(STAT1)표체정황급억제STAT1대해종류세포방사민감성적영향.방법 채용면역조직화학염색기술대비검측34례인신투명세포암표본화12례정상신조직표본적STAT1표체.용Western blotting법검측리체배양인신투명세포암세포(CRL-1932)、섬유모세포(CCL-116)화wilm's류세포(CRL-1441)적STAT1표체.용불체랍빈화siRNA억제CRL-1932세포STAT1표체,병통과성극륭법화태반람염색계수법연구억제STAT1대CRL-1932세포방사민감성적영향.결과 인신투명세포암표본적총STAT1화린산화STAT1표체균명현고우정상신조직.Western blotting법현시CRL-1932세포STAT1표체비CRL-1441、CCL-116세포명현증고;약물불체랍빈능현저억제CRL-1932세포린산화STAT1적표체,병현저증가CRL-1932세포적방사민감성,차방사증민정도여약물농도정정상관.경STAT1 siRNA처리후CRL-1932세포총STAT1화린산화STAT1적표체균피유효억제,차세포재조사후적존활분수현저하강.결론 신투명세포암STAT1정고표체,억제STAT1대해세포유방사증민작용.
Objective To study the expression of signal transducer and activator of transcription 1 (STAT1) in human renal clear cell carcinoma (RCC) and the effect of STATI inhibition on the radiosensi-tivity of RCC. Methods The expression of STAT1 in 34 human RCC samples compared with 12 normal kid-ney tissues was examined by immunohistochemistry method. For in vitro experiments, a human RCC cell line, CRL-1932, was used. Western blotting was performed to evaluate the expression of total and phospory-lated STAT1. Fludarabine and siRNA were respectively used to inhibit the expression of STAT1 in CRL-1932 cells. Clonogenic assay and trypan blue staining assay were used to evaluate the radiosensitivity of CRL-1932 cells. Results The expression of both total and phospborylated STAT1 in human RCC samples was signifi-cantly higher when compared to normal kidney tissues. Similarly, the expression of STAT1 was higher in CRL-1932 cells when compared to fibroblast and Wilm's tumor cell lines. STAT1 expression was inhibited by both fludarabine and siRNA. Radiosensitivity of CRL-1932 cells was enhanced by both fludarabine and siRNA induced STAT1 inhibition. Conclusions STAT1 is over-expressed in both human RCC tissue and cell line. Inhibition of STAT1 can enhance the radiosensitivity of RCC cells.
