CAJ | 학술논문

目的研究淋病奈瑟菌表面蛋白A(NspA)的抗原性及其在淋病快速诊断技术中的应用价值.方法用PCR技术克隆淋病奈瑟菌NspA基因,构建其原核表达载体,在大肠杆菌中表达重组NspA,表达产物复性后免疫小鼠,Western blot、ELISA分别检测免疫血清与淋病奈瑟菌细胞裂解液中NspA及与淋病奈瑟菌全细胞的结合能力.结果 NspA基因可在大肠杆菌中表达,纯化并复性的重组NspA(rrNspA)可在小鼠体内刺激产生高效价特异性抗体,抗rrNspA抗体可与淋病奈瑟菌完整细胞及细胞裂解液中NspA特异性结合.结论获得的rrNspA及抗rrNspA抗体在建立淋病奈瑟菌抗体或抗原快速检测方法中具有潜在的应用价值.
목적 연구임병내슬균표면단백A(NspA)적항원성급기재임병쾌속진단기술중적응용개치.방법 용PCR기술극륭임병내슬균NspA기인,구건기원핵표체재체,재대장간균중표체중조NspA,표체산물복성후면역소서,Western blot、ELISA분별검측면역혈청여임병내슬균세포렬해액중NspA급여임병내슬균전세포적결합능력.결과 NspA기인가재대장간균중표체,순화병복성적중조NspA(rrNspA)가재소서체내자격산생고효개특이성항체,항rrNspA항체가여임병내슬균완정세포급세포렬해액중NspA특이성결합.결론 획득적rrNspA급항rrNspA항체재건립임병내슬균항체혹항원쾌속검측방법중구유잠재적응용개치.
Objective To develop fast detection techniques for the diagnosis of gonococcal infections.Methods Prokaryotic expression vector for Neisserial surface protein A (NspA) was constructed using the NspA gene cloned by PCR.Mice were immunized with the renatured recombinant NspA (rrNspA)to prepare antibodies against NspA.Western blot and ELISA was used to analyze the binding of NspA antibodies to lysate of gonococcal cells or to intact gonococci.Results NspA antibodies that were prepared by the rrNspA expressed in E.coli could bind to rrNspA,the natural NspA existing in gonococcal cells,or intact gonococci.Conclusion RrNspA and its antibodies have potential value in developing fast diagnostic kits for gonococcal diseases.