CAJ | 학술논문

目的:观察在体外共培养系统中RPE对Müller细胞的影响.方法:Transwell小室共培养RPE和Müller细胞,MTT法、细胞计数法,测定Müller增殖和迁移的情况.结果:Müller 细胞增殖的实验:Müller 细胞的增殖,除了3h与6h,24h与48h之外,在其他各时间点之间差别有统计学意义.Müller细胞与RPE共培养组和Müller细胞单独培养组两组之间差别有统计学意义.Müller 细胞迁移的实验:Müller 细胞迁移,除了3h和6h之外,在其他各时间点之间差别有统计学意义.Müller细胞与RPE共培养组和Müller细胞单独培养组两组之间差别有统计学意义.在析因设计实验中,与RPE共培养、缺氧条件,这两个因素都可以分别作为独立的因素促进Müller细胞的增殖和迁移,而两者不存在协同作用.结论:正常和缺氧条件下RPE促进Müller的增殖、迁移,随共培养时间的延长RPE促进Müller增殖、迁移的作用加强.缺氧条件下RPE与Müller细胞间的相互作用在视网膜增殖性疾病中起到了重要的作用.
목적:관찰재체외공배양계통중RPE대Müller세포적영향.방법:Transwell소실공배양RPE화Müller세포,MTT법、세포계수법,측정Müller증식화천이적정황.결과:Müller 세포증식적실험:Müller 세포적증식,제료3h여6h,24h여48h지외,재기타각시간점지간차별유통계학의의.Müller세포여RPE공배양조화Müller세포단독배양조량조지간차별유통계학의의.Müller 세포천이적실험:Müller 세포천이,제료3h화6h지외,재기타각시간점지간차별유통계학의의.Müller세포여RPE공배양조화Müller세포단독배양조량조지간차별유통계학의의.재석인설계실험중,여RPE공배양、결양조건,저량개인소도가이분별작위독립적인소촉진Müller세포적증식화천이,이량자불존재협동작용.결론:정상화결양조건하RPE촉진Müller적증식、천이,수공배양시간적연장RPE촉진Müller증식、천이적작용가강.결양조건하RPE여Müller세포간적상호작용재시망막증식성질병중기도료중요적작용.
AIM: To investigate the role of retinal pigment epithelium (RPE) in the growth of Müller cells using a co-culture system in vitro . METHODS: Müller cells were cocultured with RPE cells under both normoxic and hypoxic conditions in Transwell chamber culture system. Müller cell proliferation was evaluated by MTT assay. The number of cells which migrate through micropores and stay on the outer bottom side of insert systems were observed and counted. RESULTS: The activities of proliferation and migration of Müller cells when cocultured with RPE cells were significantly higher than those of the Müller cells when cultured alone at all time points under both normoxic and hypoxic conditions. However, for both the coculture and control groups, there is no significant difference between the measurements at 3 and 6 hours. CONCLUSION: Evidence suggests that RPE, when co-cultured with Müller cells, can stimulate migration and proliferation of Müller cells under both hypoxic and normoxic conditions in a time-dependent manner; how-ever, there is no evidence to support the synergetic interaction of RPE and Müller cells co-cultured under hypoxic conditions.