国际检验医学杂志
國際檢驗醫學雜誌
국제검험의학잡지
INTERNATIONAL JOURNAL OF LABORATORY MEDICINE
2008年
5期
385-387,391
,共4页
姜伟%王开正%白克镇%周明术%代碧珍%蔡美珠
薑偉%王開正%白剋鎮%週明術%代碧珍%蔡美珠
강위%왕개정%백극진%주명술%대벽진%채미주
精神分裂症%应激障碍,创伤性,急性%抑郁症%血蛋白质类%质谱分析法
精神分裂癥%應激障礙,創傷性,急性%抑鬱癥%血蛋白質類%質譜分析法
정신분렬증%응격장애,창상성,급성%억욱증%혈단백질류%질보분석법
Schizophrenia%Stress Disorders,Traumatic,Acute%Depressive Disorder%Blood Proteins%Mass Spectrometry
目的 研究精神分裂症、急性应激障碍、抑郁症患者血清蛋白质谱的表达情况,筛选差异表达的标志蛋白并探讨其临床价值.方法 采用表面增强激光解吸电离飞行时间质谱技术(SELDI-TOF-MS)对122例精神分裂症、急性应激障碍、抑郁症患者及健康患者血清蛋白质谱图进行检测,用Biomarker Wizard 3.1版本软件分析质谱数据,筛选血清差异表达的标志蛋白并评价其临床价值.结果 精神分裂症患者与健康者血清有56个差异蛋白质峰,其中5个蛋白质峰作为标志蛋白具有潜在的临床价值;精神分裂症与急性应激障碍者之间差异表达的蛋白质峰有15个;精神分裂症与抑郁症患者血清有21个差异蛋白质峰.结论 精神分裂症、急性应激障碍、抑郁症患者血清中具有差异表达的标志蛋白,可能对其鉴别诊断和病因学研究,以及进一步研究蛋白质组学改变及其临床应用具有重要意义.
目的 研究精神分裂癥、急性應激障礙、抑鬱癥患者血清蛋白質譜的錶達情況,篩選差異錶達的標誌蛋白併探討其臨床價值.方法 採用錶麵增彊激光解吸電離飛行時間質譜技術(SELDI-TOF-MS)對122例精神分裂癥、急性應激障礙、抑鬱癥患者及健康患者血清蛋白質譜圖進行檢測,用Biomarker Wizard 3.1版本軟件分析質譜數據,篩選血清差異錶達的標誌蛋白併評價其臨床價值.結果 精神分裂癥患者與健康者血清有56箇差異蛋白質峰,其中5箇蛋白質峰作為標誌蛋白具有潛在的臨床價值;精神分裂癥與急性應激障礙者之間差異錶達的蛋白質峰有15箇;精神分裂癥與抑鬱癥患者血清有21箇差異蛋白質峰.結論 精神分裂癥、急性應激障礙、抑鬱癥患者血清中具有差異錶達的標誌蛋白,可能對其鑒彆診斷和病因學研究,以及進一步研究蛋白質組學改變及其臨床應用具有重要意義.
목적 연구정신분렬증、급성응격장애、억욱증환자혈청단백질보적표체정황,사선차이표체적표지단백병탐토기림상개치.방법 채용표면증강격광해흡전리비행시간질보기술(SELDI-TOF-MS)대122례정신분렬증、급성응격장애、억욱증환자급건강환자혈청단백질보도진행검측,용Biomarker Wizard 3.1판본연건분석질보수거,사선혈청차이표체적표지단백병평개기림상개치.결과 정신분렬증환자여건강자혈청유56개차이단백질봉,기중5개단백질봉작위표지단백구유잠재적림상개치;정신분렬증여급성응격장애자지간차이표체적단백질봉유15개;정신분렬증여억욱증환자혈청유21개차이단백질봉.결론 정신분렬증、급성응격장애、억욱증환자혈청중구유차이표체적표지단백,가능대기감별진단화병인학연구,이급진일보연구단백질조학개변급기림상응용구유중요의의.
Objective To study the expression statue of mass spectrum of serum protein from patients with schizophrenia, acute stress disorder and depressive disorder, screen differential expressed protein, and to explore its clinical significance.Methods Surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) was applied to detecting serum protein mass chromatogram for 122 subjects including 84 patients with schizophrenia, acute stress disorder, or depressive disorder and 38 healthy controls. Biomarker Wizard 3.1 software was adopted to analyze mass spectrum data, screen differential expressed markers, and further to evaluate its clinical value.Results Totally 56 differential expressed protein peaks were detected in patients with schizophrenia and healthy controls, in which 5 specific marker peaks had potential clinical value.In the groups of schizophrenia and acute stress disorder, 15 differential expressed protein peaks were detected. While in the groups of schizophrema and depressive disorder, 21 differential expressed protein peaks were obtained.Conclusion There exist differential expressed protein markers in sera from patients with schizophrenia, acute stress disorder and depressive disorder, which is of important clinical value in their differential diagnosis, etiological study, proteomic research and its clinical application.
