CAJ | 학술논문

以科丰1号×南农1138-2组合衍生的184个重组自交家系(简称RIKY)和(Essex×ZDD2315) ×ZDD2315衍生的114个BC_1F_2家系(简称BIEX)为材料,对蛋白质含量、蛋油总量与油脂含量,11S、7S、11S/7S,11S-1～11S-4,7S-1～7S-6等4组16个性状利用WinQTL Cartographer Ver.2.5的复合区间作图法(CIM)、多区间作图法(MIM)和IciMapping Ver.2.0的完备区间作图法(ICIM)进行QTL分析,结果表明:(1)在RIKY和BIEX群体分别定位到17~+个和21~+个QTL,合计38~+个QTL;在RIKY有蛋白、油脂、蛋油总量QTL11个,在11S和7S亚基组上分别只有1~+和3~+个;在BIEX有前性状QTL2~+个,有后性状QTL分别9~+和6~+个;(2)两群体16个性状上均没有检测到共享的QTL,说明两群体的蛋白质有关性状具有完全不同的遗传基础;RIKY的两个亲本间蛋白、油脂和蛋油总量有明显遗传差异,但在亚基组上遗传差异不大,而BIEX则反之;(3)4组总、分性状中,两群体一致表现出蛋白、油脂和蛋油总量和11S、7S和11S/7S比值两组在总、分性状间共享QTL(共同遗传基础),而11S亚基组和7S亚基组两组性状在总、分性状间无共享的QTL;(4)蛋白质有关性状QTL定位结果和分离分析结果共同表明这类性状主效基因和微效基因均占较大比重,要考虑两者兼用的育种方法.
이과봉1호×남농1138-2조합연생적184개중조자교가계(간칭RIKY)화(Essex×ZDD2315) ×ZDD2315연생적114개BC_1F_2가계(간칭BIEX)위재료,대단백질함량、단유총량여유지함량,11S、7S、11S/7S,11S-1～11S-4,7S-1～7S-6등4조16개성상이용WinQTL Cartographer Ver.2.5적복합구간작도법(CIM)、다구간작도법(MIM)화IciMapping Ver.2.0적완비구간작도법(ICIM)진행QTL분석,결과표명:(1)재RIKY화BIEX군체분별정위도17~+개화21~+개QTL,합계38~+개QTL;재RIKY유단백、유지、단유총량QTL11개,재11S화7S아기조상분별지유1~+화3~+개;재BIEX유전성상QTL2~+개,유후성상QTL분별9~+화6~+개;(2)량군체16개성상상균몰유검측도공향적QTL,설명량군체적단백질유관성상구유완전불동적유전기출;RIKY적량개친본간단백、유지화단유총량유명현유전차이,단재아기조상유전차이불대,이BIEX칙반지;(3)4조총、분성상중,량군체일치표현출단백、유지화단유총량화11S、7S화11S/7S비치량조재총、분성상간공향QTL(공동유전기출),이11S아기조화7S아기조량조성상재총、분성상간무공향적QTL;(4)단백질유관성상QTL정위결과화분리분석결과공동표명저류성상주효기인화미효기인균점교대비중,요고필량자겸용적육충방법.
Soybean processing industry places emphasis on the quality of soybean protein which is related to the protein components, mainly 11S, 7S and 11S/7S, and their subunit constituents. In the improvement of soybean protein quality, the knowledge of genetic structure of the traits related to protein quality is of great importance. Therefore, the present paper was aimed at mapping QTLs of 16 traits, including protein content, protein plus fat content, fat content, 11S, 7S, 11S/7S, and subunit groups. Two populations, RIKY population with 184 recombinant inbred lines derived from Kefeng lxNannong 1138-2 and BIEX population with 114 BC_1F_2 lines derived from (Essex×ZDD2315)×ZDD2315, were used to map QTLs with the softwares of composite interval mapping (CIM), multiple interval mapping (MIM) of WinQTL Cartographer Ver. 2.5 and the inclusive composite interval mapping (ICIM) of IciMapping. The results showed that there were totally 17~+ QTLs detected with 11 for protein content, fat content and total content of protein and fat, and 1~+ and 3~+ for 11S subunit groups and 7S subunit groups, respectively, in RIKY, as well as totally 21~+ detected with only 2~+ for protein content, fat content and protein plus fat content, but 9~+ and 6~+ for 11S subunit groups and 7S subunit groups, respectively, in BIEX. There was no shared QTL detected for all 16 traits in both populations, indicating that the 16 traits between RIKY and BIEX have completely different genetic systems, and there existed obvious genetic differences between two parents of RIKY in protein content, fat content and protein plus fat content but less genetic differences in US subunit groups and 7S subunit groups, and those of BIEX were on the contrary. The group of protein content, fat content and protein plus fat content and the group of 11S, 7S and 11S/7S had common QTLs, showing their common genetic base, but there were no common QTLs in the groups of US subunit and 7S subunit. The results from QTL mapping and segregation analysis showed jointly that both major genes and minor genes contributed a large part of phenotypic variations for all 16 traits, suggesting that both major genes and minor genes should be considered in the breeding for protein-related traits.