激光生物学报
激光生物學報
격광생물학보
ACTA LASER BIOLOGY SINICA
2009年
6期
751-754
,共4页
Tris%碳重离子%辐照%质粒DNA%SSB%DSB
Tris%碳重離子%輻照%質粒DNA%SSB%DSB
Tris%탄중리자%복조%질립DNA%SSB%DSB
Tris%carbon beam%radiation%plasmid DNA%SSB%DSB
利用100 keV/μm碳离子束(初始能量为290 MeV/u)照射溶解于纯水、10 mmol/L Tris、1 mmol/L EDTA及TE 缓冲液中的pUC19质粒DNA.通过琼脂糖凝胶电泳技术分析了不同溶液中各种形态DNA分子所占份额,并计算得到不同剂量下平均每个质粒分子中单链断裂(SSB)及双链断裂(DSB)的数目.发现Tris通过抑制SSB和DSB的产生对碳重离子辐照下的质粒DNA有明显的保护作用,而EDTA能够加剧SSB的产生而抑制DSB的形成.
利用100 keV/μm碳離子束(初始能量為290 MeV/u)照射溶解于純水、10 mmol/L Tris、1 mmol/L EDTA及TE 緩遲液中的pUC19質粒DNA.通過瓊脂糖凝膠電泳技術分析瞭不同溶液中各種形態DNA分子所佔份額,併計算得到不同劑量下平均每箇質粒分子中單鏈斷裂(SSB)及雙鏈斷裂(DSB)的數目.髮現Tris通過抑製SSB和DSB的產生對碳重離子輻照下的質粒DNA有明顯的保護作用,而EDTA能夠加劇SSB的產生而抑製DSB的形成.
이용100 keV/μm탄리자속(초시능량위290 MeV/u)조사용해우순수、10 mmol/L Tris、1 mmol/L EDTA급TE 완충액중적pUC19질립DNA.통과경지당응효전영기술분석료불동용액중각충형태DNA분자소점빈액,병계산득도불동제량하평균매개질립분자중단련단렬(SSB)급쌍련단렬(DSB)적수목.발현Tris통과억제SSB화DSB적산생대탄중리자복조하적질립DNA유명현적보호작용,이EDTA능구가극SSB적산생이억제DSB적형성.
To probe into the effects of tris (hydroxymethyl) aminomethane on DNA irradiated by carbon beam, the plasmid pUC19 in water, 10 mmol/L Tris, 1 mmol/L EDTA or TE Buffer, was irradiated with 100 keV/μm ~(12) C~(6+) ions (original energy was 290 MeV/u). Through agarose gel electrophoresis and quantification, Tris was found to have obvious protective effects on plasmid DNA irradiated by carbon beam by inhibiting the formation of both DNA single strand breaks (SSBs) and double strand breaks (DSBs). EDTA, another component of TE Buffer, was found to promote the formation of SSBs while inhibit the formation of DSBs.
