CAJ | 학술논문

为明确改性效果,本文选择适宜的方法对香菇多糖分子结构进行了化学修饰和结构表征.选用氯磺酸-吡啶法和浓硫酸法对香菇多糖硫酸酯化,并进行了比较,利用一氯乙酸法对其进行羧甲基化,借助红外光谱和核磁共振碳谱对样品分别进行了结构表征.结果表明,氯磺酸-吡啶法硫酸化取代度达1.38,产率达79.23%,硫含量达14.59%,效果优于浓硫酸法;1247和807 cm~(-1)的特征吸收峰证明了硫酸基的引入,碳谱δ 79.06附近的峰证明了C2和C4被部分硫酸基取代;羧甲基化碳谱中C2和C4位附近出峰说明其位上羟基被取代,且异头碳为单一β-D-吡喃环糖苷键构型.通过红外光谱和核磁共振碳谱对香菇多糖结构表征证实,硫酸酯化和羧甲基化二种化学修饰方法对香菇多糖分子结构改性是可行的,且氯磺酸-吡啶法更适合香菇多糖硫酸化.
위명학개성효과,본문선택괄의적방법대향고다당분자결구진행료화학수식화결구표정.선용록광산-필정법화농류산법대향고다당류산지화,병진행료비교,이용일록을산법대기진행최갑기화,차조홍외광보화핵자공진탄보대양품분별진행료결구표정.결과표명,록광산-필정법류산화취대도체1.38,산솔체79.23%,류함량체14.59%,효과우우농류산법;1247화807 cm~(-1)적특정흡수봉증명료류산기적인입,탄보δ 79.06부근적봉증명료C2화C4피부분류산기취대;최갑기화탄보중C2화C4위부근출봉설명기위상간기피취대,차이두탄위단일β-D-필남배당감건구형.통과홍외광보화핵자공진탄보대향고다당결구표정증실,류산지화화최갑기화이충화학수식방법대향고다당분자결구개성시가행적,차록광산-필정법경괄합향고다당류산화.
The processes of sulfation modification of lentinan were conducted with chlosulfonic-pyridine and concentrated sulfuric acid methods,which were compared correspondingly.Carboxymethyl lentinans were treated with monochloroacetic acid and then the structure characterization was employed to identify the modification effect by using IR and 13C NMR.The substitution degree of it with chlosulfonic-pyridine method was better than that of concentrated sulfuric acid method with 79.23% yield and 14.59% sulfur contents.Sulfate groups were introduced successfully with the absorbance of 1247 and 807 cm-1 IR spectrum.The absorbance of δ79.06 showed that hydroxyl groups of C2 and C4 were partly substituted by sulfate groups.The absorbance nearby C2 and C4 identified that hydroxyl groups had been replaced by carboxymethyl group,which also elucidated the β-D-pyranoside configuration of its anomeric carbon.It' s feasible to modify the molecular structure of lentinan by sulfate and carboxymethyl reagents through IR and ~(13)C NMR spectral studies.