时珍国医国药
時珍國醫國藥
시진국의국약
LISHIZHEN MEDICINE AND MATERIA MEDICA RESEARCH
2010年
3期
524-526
,共3页
张凤%安利国%李福荣%赵晓民%文今福%杨桂文
張鳳%安利國%李福榮%趙曉民%文今福%楊桂文
장봉%안리국%리복영%조효민%문금복%양계문
反相高效液相色谱%表没食子儿茶素没食子酸酯%胃癌细胞%细胞凋亡
反相高效液相色譜%錶沒食子兒茶素沒食子痠酯%胃癌細胞%細胞凋亡
반상고효액상색보%표몰식자인다소몰식자산지%위암세포%세포조망
RP-HPLC%EGCG%Gastric cancer cell%Cell apoptosis
目的 分析表没食子儿茶素没食子酸酯(EGCG)在绿茶提取物中的含量,探讨EGCG对人胃癌细胞BGC823增殖和凋亡的影响,为阐明EGCG抗肿瘤作用机制提供实验基础.方法 反相高效液相色谱法(RP-HPLC)测定绿茶提取物中EGCG的含量.MTT法检测EGCG对体外培养的人胃癌细胞BGC823增殖的影响;DNA ladder法检测EGCG处理各组DNA梯度的变化.所有数据均采用SPSS12.0进行统计分析,均数比较采用t-test.结果 通过绘制标准曲线确定绿茶提取物中EGCG 的含量为9%.MTT法检测结果显示EGCG能够呈现剂量(5×10~(-3),1×10~(-2),2×10~(-2),4×10~(-2))g/L依赖性地抑制人胃癌细胞BGC823的增殖;DNA ladder法显示EGCG处理组细胞DNA呈现梯状条带,而对照组则没有这种带纹.结论 绿茶提取物中EGCG的含量为9%;EGCG能够通过诱导细胞凋亡而抑制人胃癌细胞BGC823的生长和增殖.
目的 分析錶沒食子兒茶素沒食子痠酯(EGCG)在綠茶提取物中的含量,探討EGCG對人胃癌細胞BGC823增殖和凋亡的影響,為闡明EGCG抗腫瘤作用機製提供實驗基礎.方法 反相高效液相色譜法(RP-HPLC)測定綠茶提取物中EGCG的含量.MTT法檢測EGCG對體外培養的人胃癌細胞BGC823增殖的影響;DNA ladder法檢測EGCG處理各組DNA梯度的變化.所有數據均採用SPSS12.0進行統計分析,均數比較採用t-test.結果 通過繪製標準麯線確定綠茶提取物中EGCG 的含量為9%.MTT法檢測結果顯示EGCG能夠呈現劑量(5×10~(-3),1×10~(-2),2×10~(-2),4×10~(-2))g/L依賴性地抑製人胃癌細胞BGC823的增殖;DNA ladder法顯示EGCG處理組細胞DNA呈現梯狀條帶,而對照組則沒有這種帶紋.結論 綠茶提取物中EGCG的含量為9%;EGCG能夠通過誘導細胞凋亡而抑製人胃癌細胞BGC823的生長和增殖.
목적 분석표몰식자인다소몰식자산지(EGCG)재록다제취물중적함량,탐토EGCG대인위암세포BGC823증식화조망적영향,위천명EGCG항종류작용궤제제공실험기출.방법 반상고효액상색보법(RP-HPLC)측정록다제취물중EGCG적함량.MTT법검측EGCG대체외배양적인위암세포BGC823증식적영향;DNA ladder법검측EGCG처리각조DNA제도적변화.소유수거균채용SPSS12.0진행통계분석,균수비교채용t-test.결과 통과회제표준곡선학정록다제취물중EGCG 적함량위9%.MTT법검측결과현시EGCG능구정현제량(5×10~(-3),1×10~(-2),2×10~(-2),4×10~(-2))g/L의뢰성지억제인위암세포BGC823적증식;DNA ladder법현시EGCG처리조세포DNA정현제상조대,이대조조칙몰유저충대문.결론 록다제취물중EGCG적함량위9%;EGCG능구통과유도세포조망이억제인위암세포BGC823적생장화증식.
Objective To analyze the content of Epigallocatechin gallate(EGCG)in green tea extraction and to investigate the effect of EGCG on the proliferation and apoptosis of human gastric cancer cells BGC823 cultured in vitro,which provide experimental basis for clarifying the mechanism of antitumor effect of EGCG.Methods In the condition of mobile phase double distilled water/acetonitrile/ethyl acetate (86:12:2 v/v/v), a flow rate of 1.0 ml/min,RP-HPLC Chromatogram was used to study EGCG in green tea. Human gastric cancer cells BGC823 cultured in vitro were treated with different concentrations of EGCG, and then the absorbencyon at 490 nm of the cells in each group was examined by MTT method. DNA ladder assay was used to detect the apoptosis of BGC823.Statistical analysis was performed by SPSS12.0 and t test was used to compare the mean value between the different groups.Results There was 9% EGCG in green tea extraction calculating by drawing standard cure. After treatment with different concentrations of EGCG, as measured by MTT assay ,cell viability decreased in a dose- (5×10~(-3),1×10~(-2),2×10~(-2),4×10~(-2)g/L) dependent manner(showed as figure 2). DNA ladder assay showed that DNA of the cells treated with EGCG showed the characteristics of apoptosis-DNA ladder,while the control group did not have such strip.Conclusion There was 9% EGCG in green tea.EGCG can inhibit the growth and proliferation of gastric cancer cells BGC823 by inducing its apoptosis.