中国组织工程研究与临床康复
中國組織工程研究與臨床康複
중국조직공정연구여림상강복
JOURNAL OF CLINICAL REHABILITATIVE TISSUE ENGINEERING RESEARCH
2010年
14期
2485-2491
,共7页
周长辉%田毅%杨波%胡祥%焦红亮%周云帆%王成春%谷晨熙%雷宁静%关方霞
週長輝%田毅%楊波%鬍祥%焦紅亮%週雲帆%王成春%穀晨熙%雷寧靜%關方霞
주장휘%전의%양파%호상%초홍량%주운범%왕성춘%곡신희%뢰저정%관방하
沃顿胶间充质干细胞%T淋巴细胞%免疫调节%γ-干扰素%转化生长因子β1
沃頓膠間充質榦細胞%T淋巴細胞%免疫調節%γ-榦擾素%轉化生長因子β1
옥돈효간충질간세포%T림파세포%면역조절%γ-간우소%전화생장인자β1
背景:骨髓源性间充质干细胞具有低免疫原性和免疫调节作用,但有关人脐带沃顿胶间充质干细胞的免疫调节作用及其机制研究较少.目的:观察脐带沃顿胶间充质干细胞对异体外周血T淋巴细胞的免疫调节作用及机制.方法:从人脐带沃顿胶组织块培养中分离出间充质干细胞.将脐带沃顿胶间充质干细胞及脐带沃顿胶间充质干细胞培养上清分别与植物凝集素刺激下的人外周血T淋巴细胞共培养,多功能酶标仪测量脐带沃顿胶间充质干细胞及脐带沃顿胶间充质干细胞培养上清与植物凝集素刺激下的人外周血T淋巴细胞共培养72 h后A值:ELISA试剂盒检测培养上清中转化生长因子β1、γ-干扰素的表达.结果与结论:沃顿胶间充质干细胞对植物凝集素刺激下的T淋巴细胞增殖有抑制作用,增殖抑制率为56%(P<0.01),沃顿胶间充质干细胞培养上清对植物凝集素刺激下的T淋巴细胞增殖也具有抑制作用,且这种抑制呈剂量依赖性.含50%沃顿胶间充质干细胞培养上清组和100%培养上清组的增殖抑制率分别为8.3%、27%(P<0.05);沃顿胶间充质干细胞能显著降低T淋巴细胞分泌的γ-干扰素(P<0.05):可分泌转化生长因子β1,沃顿胶间充质干细胞与T淋巴细胞共培养组的转化生长因子β1分泌量低于沃顿胶间充质干细胞单独培养组(P<0.05).提示沃顿胶间充质干细胞及沃顿胶间充质干细胞培养上清对植物凝集素刺激下的丁淋巴细胞增殖均有抑制作用,其作用机制可能与细胞间的接触及抑制T淋巴细胞分泌的γ-干扰素有关.
揹景:骨髓源性間充質榦細胞具有低免疫原性和免疫調節作用,但有關人臍帶沃頓膠間充質榦細胞的免疫調節作用及其機製研究較少.目的:觀察臍帶沃頓膠間充質榦細胞對異體外週血T淋巴細胞的免疫調節作用及機製.方法:從人臍帶沃頓膠組織塊培養中分離齣間充質榦細胞.將臍帶沃頓膠間充質榦細胞及臍帶沃頓膠間充質榦細胞培養上清分彆與植物凝集素刺激下的人外週血T淋巴細胞共培養,多功能酶標儀測量臍帶沃頓膠間充質榦細胞及臍帶沃頓膠間充質榦細胞培養上清與植物凝集素刺激下的人外週血T淋巴細胞共培養72 h後A值:ELISA試劑盒檢測培養上清中轉化生長因子β1、γ-榦擾素的錶達.結果與結論:沃頓膠間充質榦細胞對植物凝集素刺激下的T淋巴細胞增殖有抑製作用,增殖抑製率為56%(P<0.01),沃頓膠間充質榦細胞培養上清對植物凝集素刺激下的T淋巴細胞增殖也具有抑製作用,且這種抑製呈劑量依賴性.含50%沃頓膠間充質榦細胞培養上清組和100%培養上清組的增殖抑製率分彆為8.3%、27%(P<0.05);沃頓膠間充質榦細胞能顯著降低T淋巴細胞分泌的γ-榦擾素(P<0.05):可分泌轉化生長因子β1,沃頓膠間充質榦細胞與T淋巴細胞共培養組的轉化生長因子β1分泌量低于沃頓膠間充質榦細胞單獨培養組(P<0.05).提示沃頓膠間充質榦細胞及沃頓膠間充質榦細胞培養上清對植物凝集素刺激下的丁淋巴細胞增殖均有抑製作用,其作用機製可能與細胞間的接觸及抑製T淋巴細胞分泌的γ-榦擾素有關.
배경:골수원성간충질간세포구유저면역원성화면역조절작용,단유관인제대옥돈효간충질간세포적면역조절작용급기궤제연구교소.목적:관찰제대옥돈효간충질간세포대이체외주혈T림파세포적면역조절작용급궤제.방법:종인제대옥돈효조직괴배양중분리출간충질간세포.장제대옥돈효간충질간세포급제대옥돈효간충질간세포배양상청분별여식물응집소자격하적인외주혈T림파세포공배양,다공능매표의측량제대옥돈효간충질간세포급제대옥돈효간충질간세포배양상청여식물응집소자격하적인외주혈T림파세포공배양72 h후A치:ELISA시제합검측배양상청중전화생장인자β1、γ-간우소적표체.결과여결론:옥돈효간충질간세포대식물응집소자격하적T림파세포증식유억제작용,증식억제솔위56%(P<0.01),옥돈효간충질간세포배양상청대식물응집소자격하적T림파세포증식야구유억제작용,차저충억제정제량의뢰성.함50%옥돈효간충질간세포배양상청조화100%배양상청조적증식억제솔분별위8.3%、27%(P<0.05);옥돈효간충질간세포능현저강저T림파세포분비적γ-간우소(P<0.05):가분비전화생장인자β1,옥돈효간충질간세포여T림파세포공배양조적전화생장인자β1분비량저우옥돈효간충질간세포단독배양조(P<0.05).제시옥돈효간충질간세포급옥돈효간충질간세포배양상청대식물응집소자격하적정림파세포증식균유억제작용,기작용궤제가능여세포간적접촉급억제T림파세포분비적γ-간우소유관.
BACKGROUND:Bone marrow mesenchymal stem cells have low immunogenicity and immunomodulatory effect,but there are seldom reports concerning the immunomodulatory effect of Wharton's jelly-derived mesenchymal stem cells of human umbilical cord and its mechanims.OBJECTIVE:To investigate the immunomodulatory effects and mechanisms of Wharton's jelly-derived mesenchymal stem cells of human umbilical cord on varient peripheral blood T lymphocytes.METHODS:Mesenchymal stem cells were isolateded from Wharton's jelly of human umbilical cord by tissue culture.T lymphocytes from human peripheral blood were stimulated by phytohemagglutinin and co-cultured with umbilical cord Wharton's jelly-derived mesenchymal stem cells and umbilical cord Wharton's jelly-derived mesenchymal stem cells supernatant respectively to measure A value following 72 hours of coculture using multifunctional microplate reader.Expression of cytokines including transforming growth factor-beta 1(TGF-β1)and interferon-y(IFN-γ)was evaluated by enzyme-labeled immunosorbent assay.RESULTS AND CONCLUSION:Wharton's jelly-derived mesenchymal stem cells could inhibite the proliferation of T lymphocytes induced by phytohemagglutinin.The proliferation inhibition rate was 56%(P<0.01).Wharton's jelly-derived mesenchymal stem cells supernatant also had inhibitory effects on proliferation of T lymphocytes induced by phytohemagglutinin,in a dose-dependent fashion.The proliferation inhibition rates were 8.3% and 27% respectively in the 50% Wharton's jelly-derived mesenchymal stem cells supernatant and 100% Wharton's jelly-derived mesenchymal stem cells supematant groups(P<0.05).Wharton's jelly-derived mesenchymal stem cells significantly decreased γ-interferon secrted from T-lymphocytes(P<0.05).The secretion of TGF-β1 was lower in the coculture of Wharton's jelly-derived mesenchymal stem cells and T lymphocytes group than Wharton's jelly-derived mesenchymal stem cells alone group(P<0.05).These indicated that Wharton's jelly-derived mesenchymal stem cells and Wharton's jelly-derived mesenchymal stem cells supernatant have inhibitory effects on proliferation of T lymphocytes induced by phytohemagglutinin.The mechanims may be associated with cell contant and inhibition of v-interferon secrted from T-lymphocytes.