CAJ | 학술논문

目的:观察黄芪皂甙和丹参酮ⅡA注射液对大鼠骨骼肌钝挫伤后修复过程中氧化应激的影响.方法:120只雄性SD大鼠随机分为黄芪皂甙组、丹参酮ⅡA注射液组、黄芪皂甙+丹参酮ⅡA注射液组、黄芪丹参注射液组、生理盐水对照组,每组24只.采用打击装置造成右侧腓肠肌中段钝挫伤模型,分别于损伤局部注射黄芪皂甙、丹参酮ⅡA注射液、黄芪皂甙+丹参酮ⅡA注射液(黄芪皂甙0.5ml+丹参酮ⅡA注射液0.5ml混合)、黄芪丹参注射液(黄芪注射液0.5m1+丹参注射液0.5ml混合)及生理盐水,注射剂量均为1ml/只/次,以后每隔3天注射一次,并于损伤后1、4、7和14天取胖肠肌测定丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性、谷胱甘肽过氧化物酶(GSH-px)活性.结果:与生理盐水对照组相比,丹参酮ⅡA注射液组、黄芪皂甙+丹参酮ⅡA注射液组、黄芪丹参注射液组MDA含量在第4天时显著降低(P<0.05),而黄芪皂甙组下降不显著;与生理盐水对照组相比,黄芪皂甙组、丹参酮ⅡA注射液组、黄芪皂甙+丹参酮ⅡA注射液组、黄芪丹参注射液组SOD、GSH-px活性在第4天时显著升高(P<0.05),黄芪皂甙组升高幅度低于丹参酮ⅡA注射液组、黄芪皂甙+丹参酮ⅡA注射液组、黄芪丹参注射液组,与黄芪丹参注射液组相比,丹参酮ⅡA注射液组、黄芪皂甙+丹参酮ⅡA注射液组SOD,GSH-px活性无显著差异(P>0.05).结论:黄芪皂甙和丹参酮ⅡA注射液均可减轻骨骼肌钝挫伤后氧化应激的损伤,并提高骨骼肌SOD和GSH-px的活性,而丹参酮ⅡA注射液作用更为显著.
목적:관찰황기조대화단삼동ⅡA주사액대대서골격기둔좌상후수복과정중양화응격적영향.방법:120지웅성SD대서수궤분위황기조대조、단삼동ⅡA주사액조、황기조대+단삼동ⅡA주사액조、황기단삼주사액조、생리염수대조조,매조24지.채용타격장치조성우측비장기중단둔좌상모형,분별우손상국부주사황기조대、단삼동ⅡA주사액、황기조대+단삼동ⅡA주사액(황기조대0.5ml+단삼동ⅡA주사액0.5ml혼합)、황기단삼주사액(황기주사액0.5m1+단삼주사액0.5ml혼합)급생리염수,주사제량균위1ml/지/차,이후매격3천주사일차,병우손상후1、4、7화14천취반장기측정병이철(MDA)함량、초양화물기화매(SOD)활성、곡광감태과양화물매(GSH-px)활성.결과:여생리염수대조조상비,단삼동ⅡA주사액조、황기조대+단삼동ⅡA주사액조、황기단삼주사액조MDA함량재제4천시현저강저(P<0.05),이황기조대조하강불현저;여생리염수대조조상비,황기조대조、단삼동ⅡA주사액조、황기조대+단삼동ⅡA주사액조、황기단삼주사액조SOD、GSH-px활성재제4천시현저승고(P<0.05),황기조대조승고폭도저우단삼동ⅡA주사액조、황기조대+단삼동ⅡA주사액조、황기단삼주사액조,여황기단삼주사액조상비,단삼동ⅡA주사액조、황기조대+단삼동ⅡA주사액조SOD,GSH-px활성무현저차이(P>0.05).결론:황기조대화단삼동ⅡA주사액균가감경골격기둔좌상후양화응격적손상,병제고골격기SOD화GSH-px적활성,이단삼동ⅡA주사액작용경위현저.