中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND OCCUPATIONAL DISEASES
2010年
1期
21-24
,共4页
邹吉敏%袁宝军%王冬梅%李超%吴俊艳
鄒吉敏%袁寶軍%王鼕梅%李超%吳俊豔
추길민%원보군%왕동매%리초%오준염
矽肺%基质金属蛋白酶%基质金属蛋白酶抑制因子
矽肺%基質金屬蛋白酶%基質金屬蛋白酶抑製因子
석폐%기질금속단백매%기질금속단백매억제인자
Silicosis%Matrix metalloproteinases%Tissue inhibitors of metaslloproteinases
目的 探讨煤工尘肺患者血清中基质金属蛋白酶(MMP-9)及基质金属蛋白酶抑制剂(TIMP-9)水平的变化及意义.方法 采用双抗体夹心ELISA法检测188例煤工尘肺(煤肺组53例,煤矽肺组67例,矽肺组68例)、57例0期(0~+期组)、64例接尘对照组和50例健康对照组血清中MMP-9及TIMP-9含量;分析血清中MMP-9和TIMP-9水平在各煤工尘肺组及各对照组间的变化,以及二者的相关性;并根据煤工尘肺期别、是否有并发症以及接尘年限等因素进行分析.结果 煤肺组血清中MMP-9水平为17.16 ng/ml、煤矽肺组为15.14ng/ml,矽肺组为17.50 ng/ml,与健康对照组比较,差异均有统计学意义(P<0.05)与接尘对照组、0~+组比较,均呈降低趋势,其中矽肺组及煤矽肺组差异有统计学意义(P<0.05).3组煤工尘肺患者血清中MMP-9水平比较,差异无统计学意义(P>0.05);煤肺组、煤矽肺组和矽肺组血清中TIMP-9水平分别为(330.00±108.42),(312.044±120.09),(366.81±135.50)ng/ml,均低于健康对照组,其中矽肺组和煤肺组血清中TIMP-9水平较接尘对照组、1~+组明显增高,差异有统计学意义(P<0.05),3组煤工尘肺组间比较,矽肺组血清中TIMP-9水平较煤矽肺组升高,差异有统计学意义(P<0.05).3组煤工尘肺患者血清中MMP-9和TIMP-9水平按不同的疾病分期、是否有并发症以及接尘年限进行分析,差异均无统计学意义(P>0.05);矽肺组血清中TIMP-9水平与年龄呈正相关(r=0.249,P<0.05),血清中MMP-9和TIMP-9水平在接尘对照组中呈正相关(r=0.294,P<0.05),其他组中未发现相关性.结论 血清中MMP-9与TIMP-9水平变化与粉尘对机体的损害程度有关,MMP-9与TIMP-9共同参与了煤工尘肺的病理生理过程;动态观察0~+期及健康煤矿工人血清中MMP-9和TIMP-9水平,可为煤工尘肺的预防及早期诊断提供参考.
目的 探討煤工塵肺患者血清中基質金屬蛋白酶(MMP-9)及基質金屬蛋白酶抑製劑(TIMP-9)水平的變化及意義.方法 採用雙抗體夾心ELISA法檢測188例煤工塵肺(煤肺組53例,煤矽肺組67例,矽肺組68例)、57例0期(0~+期組)、64例接塵對照組和50例健康對照組血清中MMP-9及TIMP-9含量;分析血清中MMP-9和TIMP-9水平在各煤工塵肺組及各對照組間的變化,以及二者的相關性;併根據煤工塵肺期彆、是否有併髮癥以及接塵年限等因素進行分析.結果 煤肺組血清中MMP-9水平為17.16 ng/ml、煤矽肺組為15.14ng/ml,矽肺組為17.50 ng/ml,與健康對照組比較,差異均有統計學意義(P<0.05)與接塵對照組、0~+組比較,均呈降低趨勢,其中矽肺組及煤矽肺組差異有統計學意義(P<0.05).3組煤工塵肺患者血清中MMP-9水平比較,差異無統計學意義(P>0.05);煤肺組、煤矽肺組和矽肺組血清中TIMP-9水平分彆為(330.00±108.42),(312.044±120.09),(366.81±135.50)ng/ml,均低于健康對照組,其中矽肺組和煤肺組血清中TIMP-9水平較接塵對照組、1~+組明顯增高,差異有統計學意義(P<0.05),3組煤工塵肺組間比較,矽肺組血清中TIMP-9水平較煤矽肺組升高,差異有統計學意義(P<0.05).3組煤工塵肺患者血清中MMP-9和TIMP-9水平按不同的疾病分期、是否有併髮癥以及接塵年限進行分析,差異均無統計學意義(P>0.05);矽肺組血清中TIMP-9水平與年齡呈正相關(r=0.249,P<0.05),血清中MMP-9和TIMP-9水平在接塵對照組中呈正相關(r=0.294,P<0.05),其他組中未髮現相關性.結論 血清中MMP-9與TIMP-9水平變化與粉塵對機體的損害程度有關,MMP-9與TIMP-9共同參與瞭煤工塵肺的病理生理過程;動態觀察0~+期及健康煤礦工人血清中MMP-9和TIMP-9水平,可為煤工塵肺的預防及早期診斷提供參攷.
목적 탐토매공진폐환자혈청중기질금속단백매(MMP-9)급기질금속단백매억제제(TIMP-9)수평적변화급의의.방법 채용쌍항체협심ELISA법검측188례매공진폐(매폐조53례,매석폐조67례,석폐조68례)、57례0기(0~+기조)、64례접진대조조화50례건강대조조혈청중MMP-9급TIMP-9함량;분석혈청중MMP-9화TIMP-9수평재각매공진폐조급각대조조간적변화,이급이자적상관성;병근거매공진폐기별、시부유병발증이급접진년한등인소진행분석.결과 매폐조혈청중MMP-9수평위17.16 ng/ml、매석폐조위15.14ng/ml,석폐조위17.50 ng/ml,여건강대조조비교,차이균유통계학의의(P<0.05)여접진대조조、0~+조비교,균정강저추세,기중석폐조급매석폐조차이유통계학의의(P<0.05).3조매공진폐환자혈청중MMP-9수평비교,차이무통계학의의(P>0.05);매폐조、매석폐조화석폐조혈청중TIMP-9수평분별위(330.00±108.42),(312.044±120.09),(366.81±135.50)ng/ml,균저우건강대조조,기중석폐조화매폐조혈청중TIMP-9수평교접진대조조、1~+조명현증고,차이유통계학의의(P<0.05),3조매공진폐조간비교,석폐조혈청중TIMP-9수평교매석폐조승고,차이유통계학의의(P<0.05).3조매공진폐환자혈청중MMP-9화TIMP-9수평안불동적질병분기、시부유병발증이급접진년한진행분석,차이균무통계학의의(P>0.05);석폐조혈청중TIMP-9수평여년령정정상관(r=0.249,P<0.05),혈청중MMP-9화TIMP-9수평재접진대조조중정정상관(r=0.294,P<0.05),기타조중미발현상관성.결론 혈청중MMP-9여TIMP-9수평변화여분진대궤체적손해정도유관,MMP-9여TIMP-9공동삼여료매공진폐적병리생리과정;동태관찰0~+기급건강매광공인혈청중MMP-9화TIMP-9수평,가위매공진폐적예방급조기진단제공삼고.
Objective To explore the changes and significance of matrix metaslloproteinases (MMP) -9 and tissue inhibitors of metaslloproteinases(TIMP)-9 in serum of patients with coal workers' pneumoconiosis(CWP). Methods Serum MMP-9 and TIMP-9 were determined by sandwich ELISA in 188 cases of coal workers' pneumoconiosis (including 53 cases of coal pneumoconiosis, 67 cases of coal-silica pneumoconiosis and 68 cases of silicosis), 57 cases of 0~+ miner (group 0~+), 64 cases of healthy miner (dust exposed control) and 50 healthy people (healthy control), and were further evaluated according to stage, complication, and the duration of exposure to dust in CWP group. Results The median of serum MMP-9 in coal pneumoconiosis, coal-silica pneumoconiosis and silicosis were 17.16, 15.14 and 17.50 ng/ml respectively. The levels of serum MMP-9 in silicosis and coal-silica pneumoconiosis were lower than that in dust exposed control and 0~+ group (P<0.05), and there were no differences among 3 groups of CWP. The levels of serum TIMP-9 in coal pneumoconiosis, coal-silica pneumoconiosis and silicosis were(330.00±108.42), (312.04±120.09) and (366.81±135.50) ng/ml respectively, and compared with dust exposed control and 0~+ group, it increased in silicosis and coal pneumoconiosis significantly (P<0.05). The levels of serum TIMP-9 in silicosis were higher significantly than that of coal-silica pneumoconiosis(P<0.05). No significant difference was observed in the levels of serum MMP-9 and TIMP-9 in CWP according to CWP stage, complications and the duration of exposure to dust (P>0.05). There was a positive correlation between age and the concentration of T1MP-9 in silicosis (r=0.249, P<0.05). A positive correlation was shown between the levels of serum MMP-9 and TIMP-9 only in dust exposed control (r=0.294.P<0.05). Conclusions The levels of serum MMP-9 and TIMP-9 are associated with the harmful effect to miner resulted from dust. The apparent disturbance of MMP-9 and TIMP-9 might take part in the pathogenesis of CWP. Dynamic observation of its concentration in group 0~+ and health miners might contribute to the prevention, early diagnosis and differential diagnosis of CWP.
