CAJ | 학술논문

目的探讨山东省沿海地区中国汉族男性群体的白细胞介素(IL)-1β启动子区rs1143627(-31C/T)基因多态性的分布状况及其与痛风易感性之间的关系.方法选取208例痛风患者和210名健康对照者,应用聚合酶链反应限制性片段长度多态性(RFLP)技术,检测中国汉族男性群体的IL-1β启动子区rs1143627(-31C/T)位点基因多态性与痛风发病的遗传易感性的关系.采用Hardy-Weinberg检验确认标本的群体代表性,数据分析采用χ2检验和t检验.结果痛风组中IL-1β启动子区-31C/T位点CC,CT和TT基因型分别为32.7%,43.3%和24.0%,健康对照组分别为31.9%,46.2%和21.9%,2组比较差异无统计学意义(χ2=0.427,P>0.05);2组的等位基因频率C和T间差异也无统计学意义(分别为54.3%,55.0%;45.7%,45.0%;χ2=0.038,P>0.05).经χ2检验,IL-1β基因-31C/T位点基因多态性与痛风病的危险因素无显著性关联.结论尚不能认为中国沿海地区汉族男性人群中IL-1β启动子区rs1143627(-31C/T)基因多态性与痛风有关联性.
목적 탐토산동성연해지구중국한족남성군체적백세포개소(IL)-1β계동자구rs1143627(-31C/T)기인다태성적분포상황급기여통풍역감성지간적관계.방법 선취208례통풍환자화210명건강대조자,응용취합매련반응한제성편단장도다태성(RFLP)기술,검측중국한족남성군체적IL-1β계동자구rs1143627(-31C/T)위점기인다태성여통풍발병적유전역감성적관계.채용Hardy-Weinberg검험학인표본적군체대표성,수거분석채용χ2검험화t검험.결과 통풍조중IL-1β계동자구-31C/T위점CC,CT화TT기인형분별위32.7%,43.3%화24.0%,건강대조조분별위31.9%,46.2%화21.9%,2조비교차이무통계학의의(χ2=0.427,P>0.05);2조적등위기인빈솔C화T간차이야무통계학의의(분별위54.3%,55.0%;45.7%,45.0%;χ2=0.038,P>0.05).경χ2검험,IL-1β기인-31C/T위점기인다태성여통풍병적위험인소무현저성관련.결론 상불능인위중국연해지구한족남성인군중IL-1β계동자구rs1143627(-31C/T)기인다태성여통풍유관련성.
Objective To explore gene polymorphism of the C/T genotype of rs1143627 in the promoter of IL-1β gene in male population living in the coastal area of Shandong, and thus to investigate the relationship between the gene polymorphism of IL-1β and gout. Methods A total of 208 gout patients and 210 healthy controls were enrolled. The possible association between the polymorphism of IL-1 β -3 1C/T and gout in Chinese were investigated and genotype frequencies and allelic frequencies was calculated by polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) method. Hardy-Weinberg was used to verify the representativeness of the sample. Comparisons between the groups were performed with χ2 test and t-test. Results The frequencies of CC, CT, and TT genotypes were 32.7%, 43.3% and 24.0%,respectively among gout patients, while they were 31.9%,46.2% and 21.9%, respectively among the controls.There was no statistically difference in IL-1β -31C/T genotype frequencies between gout patients and controls (χ2=0.427, P>0.05). The allele frequencies of C and T in gout cases were different from those in the controls (54.3%, 55.0%; 45.7%, 45.0%; χ2=0.038, P>0.05). Moreover, no association between IL- I β-31 C/T genotypes and risk factors for gout were observed in gout cases by χ2 test. Conclusion Results of the present study suggest that the C/T genotype of rs1143627 in the promoter of IL-1β gene is not associated with gout in male population living in the coastal area of Shandong.