CAJ | 학술논문

目的探讨蛋氨酸合成酶还原酶(MTRR)基因多态性与中青年脑梗死的关系. 方法采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)分析技术检测105例中青年脑梗死患者(郑州大学第一附属医院神经内科自2008年5月至2009年10月收治)和116例对照者MTRRA66G的基因型,应用高效液相色谱荧光法检测2组对象血浆同型半胱氨酸(Hey)水平. 结果脑梗死组和对照组MTRRA 66G基因型及等位基因频率比较差异无统计学意义(P＞0.05).将脑梗死组按是否合并高血压、糖尿病和冠心病进行分层分析,发现脑梗死无合并症组GG基因型及G等位基因频率明显高于对照组(36.4％ vs 23.3％,62.1％ vs 52.2％),但差异无统计学意义(P＞0.05)；脑梗死合并疾病组基因型及等位基因频率与对照组相比差异亦无统计学意义(P＞0.05).脑梗死合并疾病组、脑梗死无合并症组及对照组GG基因型血浆Hcy水平均明显高于从基因型,差异均有统计学意义(P＜0.05). 结论 MTRR A66G基因多态性与中青年脑梗死发病无关,但MTRR A66G基因纯合子突变可引起血浆Hcy水平明显升高.
목적 탐토단안산합성매환원매(MTRR)기인다태성여중청년뇌경사적관계. 방법 채용취합매련반응-한제성편단장도다태성(PCR-RFLP)분석기술검측105례중청년뇌경사환자(정주대학제일부속의원신경내과자2008년5월지2009년10월수치)화116례대조자MTRRA66G적기인형,응용고효액상색보형광법검측2조대상혈장동형반광안산(Hey)수평. 결과 뇌경사조화대조조MTRRA 66G기인형급등위기인빈솔비교차이무통계학의의(P＞0.05).장뇌경사조안시부합병고혈압、당뇨병화관심병진행분층분석,발현뇌경사무합병증조GG기인형급G등위기인빈솔명현고우대조조(36.4％ vs 23.3％,62.1％ vs 52.2％),단차이무통계학의의(P＞0.05)；뇌경사합병질병조기인형급등위기인빈솔여대조조상비차이역무통계학의의(P＞0.05).뇌경사합병질병조、뇌경사무합병증조급대조조GG기인형혈장Hcy수평균명현고우종기인형,차이균유통계학의의(P＜0.05). 결론 MTRR A66G기인다태성여중청년뇌경사발병무관,단MTRR A66G기인순합자돌변가인기혈장Hcy수평명현승고.
Objective To explore the relationship between methionine synthase reductase (MTRR) gene polymorphism and cerebral infarction in young and middle-aged patients. Methods The genotype of MTRR A66G was analyzed by polymerase chain reaction-restrictive fragment length polymorphism (PCR-RFLP) and the plasma homocysteine (Hcy) level was measured by high performance liquid chromatography in 105 young and middle-aged patients with cerebral infarction and 116 age-matched healthy controls. Results The genotype distribution and allele frequencies of MTRR A 66G gene between the 2 groups had no statistical significance (P＞0.05).Stratified analysis,performed according to whether cerebral infarction was complicated with hypertension,diabetes or coronary heart disease,indicated that the frequencies ofGG genotype and G allele in cerebral infarction patients without complications were obviously higher than those in controls (36.4％ vs.23.3％,62.1％ vs.52.2％),but no statistical significance was noted between them (P＞0.05).No statistical difference was observed between cerebral infarction patients with complications and controls (P＞0.05). The mean plasma Hcy level in patients and controls with GG genotype was significantly higher than that in patients and controls with AA genotype (P＜0.05). Conclusion No association between MTRR A 66G polymorphism and cerebral infarction is noted in young and middle-aged patients, while GG mutant homozygous ofMTRR A66G gene can significantly raise the plasma Hcy level.