CAJ | 학술논문

目的探讨间期荧光原位杂交技术(FISH)在急性髓系白血病(AML)M2和M3诊断中的意义.方法对初治的9例AML-M3、12例AML-M3及10例未能确定M2或M3的AML患者,应用FISH和反转录-聚合酶链反应(RT-PCR)检测AMLI/ETO和PML/RARα融合基因,协助诊断和指导治疗.结果 9例AML-M2中AMLI/ETO融合基因阳性率44.4%(4/9);12例AML-M3中PML/RARα融合基因阳性率83.3%(10/12),其中1例AML1/ETO融合基因阳性.确诊为AML-M2;10例AML中AML1/ETO融合基因阳性率30%(3/10),PML/RAR Ot融合基因阳性率50%(5/10),其余2例未检测到以上两种融合基因.结论 FISH是一种敏感、简便的分子遗传学新技术,具有高效、快速、灵敏等优点,对诊断AML的分型具有重要帮助,可进一步指导临床治疗.
목적 탐토간기형광원위잡교기술(FISH)재급성수계백혈병(AML)M2화M3진단중적의의.방법 대초치적9례AML-M3、12례AML-M3급10례미능학정M2혹M3적AML환자,응용FISH화반전록-취합매련반응(RT-PCR)검측AMLI/ETO화PML/RARα융합기인,협조진단화지도치료.결과 9례AML-M2중AMLI/ETO융합기인양성솔44.4%(4/9);12례AML-M3중PML/RARα융합기인양성솔83.3%(10/12),기중1례AML1/ETO융합기인양성.학진위AML-M2;10례AML중AML1/ETO융합기인양성솔30%(3/10),PML/RAR Ot융합기인양성솔50%(5/10),기여2례미검측도이상량충융합기인.결론 FISH시일충민감、간편적분자유전학신기술,구유고효、쾌속、령민등우점,대진단AML적분형구유중요방조,가진일보지도림상치료.
Objective To investigate the value of interphase fluorescence in situ hybridization(FISH)technique and the detection of fusion gene in the diagnosis of acute myeloid leukemia(AML)M2 and M3 Methods FISH was used to detect the AML1/ETO fusion gene and/or PML/RARα fusion gene in incipient cases including 9 AML-M2, 12 AML-M3 and 10 AML undetermined as AML-M2 or AML-M3 primarily diagnosed by routine morphology though bone marrow,cytochemical staining and immunophenotyping,which can help diagnose and guide clinical therapy.Results 4 of 9 AML-M2 cases were AML1/ETO positive.Among 12 AML-M3 cases,10 were PML/RARα positive.1 case was detected AML1/ETO fusion gene.In 10 untonfirmed M3 or M2,3 case8 showed AML1/ETO,5 showed PMIJRARot fusion gene and the rest showed neither of the genes.Conclusion As a new technique of the molecular genetics,FISH is accurate, rapid and efficient.It would be of significance not only at diagnosis of AML,but also for subsequent clinical decision-making.