CAJ | 학술논문

目的通过比较采集后6 h和72 h制备的冰冻单采血小板制品的多项质量参数,为冰冻单采血小板的制备标准提供依据.方法以美国产Trims血细胞分离机配套全密闭7 d保存袋采集的单采血小板,分别于采集后6 h和72 h制备为冰冻血小板制品,1周后复苏留样,分别检测血小板计数(PLT,MPV,PCT,PDW)、血小板粘附性、血小板P选择蛋白、PF3A、FF4、pH值、血块收缩试验(血浆法).结果采集后6 h和72 h制备的冰冻单采血小板,两者相比差异无统计学意义(t<0.01,P>0.05).单采血小板在不同保存条件下,PF3A均保持了良好的PF3活性,单采血小板在常规或冰冻保存条件下,PF4及P选择蛋白的表达均明显增加,采集后6 h和72 h制备的冰冻单采血小板相比,差异无统计学意义(t<0.01,P>0.05);单采血小板在常规保存3 d内,粘附功能和血块收缩试验没有明显变化,而冰冻保存的单采血小板这两项指标呈明显减退现象,血小板的活力下降明显.结论以美国产Trims血细胞分离机配套全密闭7 d保存袋采集的单采血小板,分别于采集后6 h和72 h制备的冰冻血小板制品,两者的体外指标分析结果相比差异无显著性,且都能有效的改善和控制急性大出血患者的出血倾向,用于抢救急性大出血患者疗效是可靠的.
목적 통과비교채집후6 h화72 h제비적빙동단채혈소판제품적다항질량삼수,위빙동단채혈소판적제비표준제공의거.방법 이미국산Trims혈세포분리궤배투전밀폐7 d보존대채집적단채혈소판,분별우채집후6 h화72 h제비위빙동혈소판제품,1주후복소류양,분별검측혈소판계수(PLT,MPV,PCT,PDW)、혈소판점부성、혈소판P선택단백、PF3A、FF4、pH치、혈괴수축시험(혈장법).결과 채집후6 h화72 h제비적빙동단채혈소판,량자상비차이무통계학의의(t<0.01,P>0.05).단채혈소판재불동보존조건하,PF3A균보지료량호적PF3활성,단채혈소판재상규혹빙동보존조건하,PF4급P선택단백적표체균명현증가,채집후6 h화72 h제비적빙동단채혈소판상비,차이무통계학의의(t<0.01,P>0.05);단채혈소판재상규보존3 d내,점부공능화혈괴수축시험몰유명현변화,이빙동보존적단채혈소판저량항지표정명현감퇴현상,혈소판적활력하강명현.결론 이미국산Trims혈세포분리궤배투전밀폐7 d보존대채집적단채혈소판,분별우채집후6 h화72 h제비적빙동혈소판제품,량자적체외지표분석결과상비차이무현저성,차도능유효적개선화공제급성대출혈환자적출혈경향,용우창구급성대출혈환자료효시가고적.
Objective To offer evidence for preparation criteria of frozen platelet preparation by means of comparison of a number of quality parameters between the frozen platelets prepared at 6 hour and 72 hour time point after collection.Methods Frozen platelet preparation was made 6 h and 72 h after collection of blood platelets gathered in bags by Trima Blood Corpuscle Machine.A week later, the frozen platelet preparation was dissolved and sampled for detection of PLT,MPV,PCT,PDW, platelet adhesiveness,P-selectin,PF3A,PF4,pH value,blood clot contractibility(clot retraction test).Results There were no significant differences in a series of quality parameters between the frozen platelets prepared at 6 hours and 72 hours after collection.Under the different conservation conditions.PF3A kept available activity.When the blood platelets were conserved under the conditions at22℃or at-80℃,the expressions of PF3A and p-Selection were increased,there was no difference of PF3A and p-Selection expression between the frozen platelets prepared at 6 hours and 72 hours after collection.During three days of routine conservation,no change appeared in the conglutinate function and clot contractibility;however,the above parameters were significantly decreased,and the activity of platelets was markedly datined under congealing.Conclusion There are no significant differences in a series of quality parameters between the frozen platelet preparation prepared with gathered blood by Trima Blood Corpuscle Machine at 6 hours and 72 hours after collection,and both of the frozen platelet preparation can effectively improve or control the hemorrhagic tendency of patients suffered urgent massive hemorrhage.