国际检验医学杂志
國際檢驗醫學雜誌
국제검험의학잡지
INTERNATIONAL JOURNAL OF LABORATORY MEDICINE
2008年
12期
1077-1079
,共3页
刘利明%陈蓉%詹鹏飞%邹志强%杨湘辉%江顺琴%陈雪丽
劉利明%陳蓉%詹鵬飛%鄒誌彊%楊湘輝%江順琴%陳雪麗
류리명%진용%첨붕비%추지강%양상휘%강순금%진설려
单采血小板%采集后6小时%72小时%冰冻血小板%质量
單採血小闆%採集後6小時%72小時%冰凍血小闆%質量
단채혈소판%채집후6소시%72소시%빙동혈소판%질량
Blood platelets gathered by machine%6 hours after collection%72 hours after collection%Frozen platelets%Quality
目的 通过比较采集后6 h和72 h制备的冰冻单采血小板制品的多项质量参数,为冰冻单采血小板的制备标准提供依据.方法 以美国产Trims血细胞分离机配套全密闭7 d保存袋采集的单采血小板,分别于采集后6 h和72 h制备为冰冻血小板制品,1周后复苏留样,分别检测血小板计数(PLT,MPV,PCT,PDW)、血小板粘附性、血小板P选择蛋白、PF3A、FF4、pH值、血块收缩试验(血浆法).结果 采集后6 h和72 h制备的冰冻单采血小板,两者相比差异无统计学意义(t<0.01,P>0.05).单采血小板在不同保存条件下,PF3A均保持了良好的PF3活性,单采血小板在常规或冰冻保存条件下,PF4及P选择蛋白的表达均明显增加,采集后6 h和72 h制备的冰冻单采血小板相比,差异无统计学意义(t<0.01,P>0.05);单采血小板在常规保存3 d内,粘附功能和血块收缩试验没有明显变化,而冰冻保存的单采血小板这两项指标呈明显减退现象,血小板的活力下降明显.结论 以美国产Trims血细胞分离机配套全密闭7 d保存袋采集的单采血小板,分别于采集后6 h和72 h制备的冰冻血小板制品,两者的体外指标分析结果相比差异无显著性,且都能有效的改善和控制急性大出血患者的出血倾向,用于抢救急性大出血患者疗效是可靠的.
目的 通過比較採集後6 h和72 h製備的冰凍單採血小闆製品的多項質量參數,為冰凍單採血小闆的製備標準提供依據.方法 以美國產Trims血細胞分離機配套全密閉7 d保存袋採集的單採血小闆,分彆于採集後6 h和72 h製備為冰凍血小闆製品,1週後複囌留樣,分彆檢測血小闆計數(PLT,MPV,PCT,PDW)、血小闆粘附性、血小闆P選擇蛋白、PF3A、FF4、pH值、血塊收縮試驗(血漿法).結果 採集後6 h和72 h製備的冰凍單採血小闆,兩者相比差異無統計學意義(t<0.01,P>0.05).單採血小闆在不同保存條件下,PF3A均保持瞭良好的PF3活性,單採血小闆在常規或冰凍保存條件下,PF4及P選擇蛋白的錶達均明顯增加,採集後6 h和72 h製備的冰凍單採血小闆相比,差異無統計學意義(t<0.01,P>0.05);單採血小闆在常規保存3 d內,粘附功能和血塊收縮試驗沒有明顯變化,而冰凍保存的單採血小闆這兩項指標呈明顯減退現象,血小闆的活力下降明顯.結論 以美國產Trims血細胞分離機配套全密閉7 d保存袋採集的單採血小闆,分彆于採集後6 h和72 h製備的冰凍血小闆製品,兩者的體外指標分析結果相比差異無顯著性,且都能有效的改善和控製急性大齣血患者的齣血傾嚮,用于搶救急性大齣血患者療效是可靠的.
목적 통과비교채집후6 h화72 h제비적빙동단채혈소판제품적다항질량삼수,위빙동단채혈소판적제비표준제공의거.방법 이미국산Trims혈세포분리궤배투전밀폐7 d보존대채집적단채혈소판,분별우채집후6 h화72 h제비위빙동혈소판제품,1주후복소류양,분별검측혈소판계수(PLT,MPV,PCT,PDW)、혈소판점부성、혈소판P선택단백、PF3A、FF4、pH치、혈괴수축시험(혈장법).결과 채집후6 h화72 h제비적빙동단채혈소판,량자상비차이무통계학의의(t<0.01,P>0.05).단채혈소판재불동보존조건하,PF3A균보지료량호적PF3활성,단채혈소판재상규혹빙동보존조건하,PF4급P선택단백적표체균명현증가,채집후6 h화72 h제비적빙동단채혈소판상비,차이무통계학의의(t<0.01,P>0.05);단채혈소판재상규보존3 d내,점부공능화혈괴수축시험몰유명현변화,이빙동보존적단채혈소판저량항지표정명현감퇴현상,혈소판적활력하강명현.결론 이미국산Trims혈세포분리궤배투전밀폐7 d보존대채집적단채혈소판,분별우채집후6 h화72 h제비적빙동혈소판제품,량자적체외지표분석결과상비차이무현저성,차도능유효적개선화공제급성대출혈환자적출혈경향,용우창구급성대출혈환자료효시가고적.
Objective To offer evidence for preparation criteria of frozen platelet preparation by means of comparison of a number of quality parameters between the frozen platelets prepared at 6 hour and 72 hour time point after collection.Methods Frozen platelet preparation was made 6 h and 72 h after collection of blood platelets gathered in bags by Trima Blood Corpuscle Machine.A week later, the frozen platelet preparation was dissolved and sampled for detection of PLT,MPV,PCT,PDW, platelet adhesiveness,P-selectin,PF3A,PF4,pH value,blood clot contractibility(clot retraction test).Results There were no significant differences in a series of quality parameters between the frozen platelets prepared at 6 hours and 72 hours after collection.Under the different conservation conditions.PF3A kept available activity.When the blood platelets were conserved under the conditions at22℃or at-80℃,the expressions of PF3A and p-Selection were increased,there was no difference of PF3A and p-Selection expression between the frozen platelets prepared at 6 hours and 72 hours after collection.During three days of routine conservation,no change appeared in the conglutinate function and clot contractibility;however,the above parameters were significantly decreased,and the activity of platelets was markedly datined under congealing.Conclusion There are no significant differences in a series of quality parameters between the frozen platelet preparation prepared with gathered blood by Trima Blood Corpuscle Machine at 6 hours and 72 hours after collection,and both of the frozen platelet preparation can effectively improve or control the hemorrhagic tendency of patients suffered urgent massive hemorrhage.