CAJ | 학술논문

背景:自体松质骨因具有促进骨再生的3大要素:良好的骨引导性、骨诱导件和骨细胞,目前仍然足骨缺损修复的最佳移植材料.目的:观察自主研发的仿松质骨纳米人工骨修复股骨头坏死的效果.设计、时间及地点:随机对照动物实验,于2007-05/08在广东省医学实验动物中心完成.材料:新西兰大白兔10只,体质量1.5～2.0 kg,用注入液氮的方法制备股骨头坏死模型.方法:10只大白兔随机分为2组,每组5只.实验组:骨坏死处植入HA/CO纳米人工骨,对照组:坏死处不加任何植入物.主要观察指标:在植入材料即刻及之后的7,14 d摄X射线观察、大体积及组织学观察评判骨坏死的发展及治愈情况.结果:实验组骨坏死破抑制并被修复,所钻骨孔因松质骨和皮质骨的再生而完全愈合,纳米人工骨被逐渐降解吸收;而空白对照组骨坏死逐渐恶化,所钻骨孔处无任何骨组织再生.结论:该纳米人工骨不仅能替代自体骨作为移植物,而且还具有抑制骨坏死发展的功能,可望成为临床治疗Ⅱ期及较早期股骨头坏死的替代疗法.
배경:자체송질골인구유촉진골재생적3대요소:량호적골인도성、골유도건화골세포,목전잉연족골결손수복적최가이식재료.목적:관찰자주연발적방송질골납미인공골수복고골두배사적효과.설계、시간급지점:수궤대조동물실험,우2007-05/08재광동성의학실험동물중심완성.재료:신서란대백토10지,체질량1.5～2.0 kg,용주입액담적방법제비고골두배사모형.방법:10지대백토수궤분위2조,매조5지.실험조:골배사처식입HA/CO납미인공골,대조조:배사처불가임하식입물.주요관찰지표:재식입재료즉각급지후적7,14 d섭X사선관찰、대체적급조직학관찰평판골배사적발전급치유정황.결과:실험조골배사파억제병피수복,소찬골공인송질골화피질골적재생이완전유합,납미인공골피축점강해흡수;이공백대조조골배사축점악화,소찬골공처무임하골조직재생.결론:해납미인공골불부능체대자체골작위이식물,이차환구유억제골배사발전적공능,가망성위림상치료Ⅱ기급교조기고골두배사적체대요법.
BACKGROUND: Autologous cancellous bone graft remains the most effective grafting material for bone defects because it provides the three elements required for bone regeneration: osteoconduction, osteoinduction, and osteogenic cell. OBJECTIVE: To determine the indications for a cancellous bone like nano-hydroxyapatite/collagen (nano-HA/CO) composite for treatment of osteonecrosis of the femoral head (ONFH). DESIGN, TIME AND SETTING: A randomized controlled animal study was performed at Guangdong Medical Laboratory Animal Center from May to August 2007. MATERIALS: Ten New-zealand rabbits, weighing 1.5-2.0 kg, were prepared into models of ONFH by injection of liquid nitrogen. METHODS: Ten rabbits were randomly, evenly divided into 2 groups: nano-HA/CO composites group, insertion of nano-HA/CO bone substitutes into bone defects, and control group, without any implantation. MAIN OUTCOME MEASURES: The healing processes were assessed with macroscopic examination, X-ray photography and histological examination at days 7 and 14 after surgery. RESULTS: In the nano-HA/CO composites group, osteonecrosis was halted, holes were repaired, and nano-HA/CO bone substitute was gradually degraded and absorbed; but in the control group, osteonecrosis was deteriorated and holes were not repaired, remained the same drilled at the beginning. CONCLUSION: These nano-HA/CO composites can not only replace autologous cancellous bone, but also can halt the progress of osteonecrosis insulted by liquid nitrogen. Experimental results show potent alternative of this nano-HA/CO composites in treatment of ONFH in stage II and earlier stage.