CAJ | 학술논문

目的探讨人胎盘提取液对高脂饮食性脂肪性肝病的治疗作用及其机理.方法 42只Wistar大鼠,雄性,分为正常对照组(n=8,予普通标准饲料)和模型组(n=34,予高脂饮食,建立大鼠脂肪肝模型)喂饲12周后,将模型组随机分为造模组(n=8)、人胎盘提取液高剂量组(n=9)、人胎盘提取液低剂量组(n=9)、易善复对照组(n=8).4周后取血和肝组织.肝组织HE染色观察病理学改变,用放免法检测各组大鼠血浆及肝组织中Leptin、TNF-α、IL-6的含量.结果人胎盘提取液低剂量组和易善复组较造模组有好转,但无明显差异(P>0.05),人胎盘提取液高剂量组脂肪浸润状态较造模组有明显好转,且差异有统计学意义(P<0.05).造模组大鼠血浆中Leptin、TNF-α、IL-6的含量均高于正常对照组(P<0.05),人胎盘提取液高、低剂量组、易善复对照组较造模组明显降低(P<0.05);血浆及肝组织溶浆中IL-6,TNF-α及Leptin的含量之间均无显著相关性.结论人胎盘提取液对Leptin、TNF-α、IL-6等细胞因子具有明显的抑制作用,对高脂饮食脂肪性肝病动物模型具有明显的治疗作用.
목적 탐토인태반제취액대고지음식성지방성간병적치료작용급기궤리.방법 42지Wistar대서,웅성,분위정상대조조(n=8,여보통표준사료)화모형조(n=34,여고지음식,건립대서지방간모형)위사12주후,장모형조수궤분위조모조(n=8)、인태반제취액고제량조(n=9)、인태반제취액저제량조(n=9)、역선복대조조(n=8).4주후취혈화간조직.간조직HE염색관찰병이학개변,용방면법검측각조대서혈장급간조직중Leptin、TNF-α、IL-6적함량.결과 인태반제취액저제량조화역선복조교조모조유호전,단무명현차이(P>0.05),인태반제취액고제량조지방침윤상태교조모조유명현호전,차차이유통계학의의(P<0.05).조모조대서혈장중Leptin、TNF-α、IL-6적함량균고우정상대조조(P<0.05),인태반제취액고、저제량조、역선복대조조교조모조명현강저(P<0.05);혈장급간조직용장중IL-6,TNF-α급Leptin적함량지간균무현저상관성.결론 인태반제취액대Leptin、TNF-α、IL-6등세포인자구유명현적억제작용,대고지음식지방성간병동물모형구유명현적치료작용.
Objective To investigate the effects and mechanisms of human placenta extracts to rats with nonalcoholic fatty liver disease.Methods Forty-two male Wistar rats were randomly divided into two groups: blank control group (n=8, fed by ordinary standard diet) and fatty-liver group (n=34, the fatty-liver models were established by feeding with high-fatty diet by a term of 12-week).Then the fatty-liver models were randomly scarified for model group (n=8),human placenta high dose group (n=9), human placenta low dose group (n=9) and Essentiale group (n=8).By the end of 4th week, histological examinations of liver were carried out with HE staining; meanwhile, the serum and tissue levels of Leptin、TNF-α、IL-6 were detected with RIA.Results The fatty degeneration in livers with low dose human placenta extracts and Essentiale were slightly milder than that of the models(P>0.05).While high dose human placenta could obviously lessen the fatty degeneration (P<0.05).Compared with the blank control group,the serum and tissue levels of Leptin、TNF-αand IL -6 in the model group were all remarkably increased(P<0.05). And the human placenta and Essentiale could typical relevance between the contents of Leptin、TNF-α and IL -6 both in serum and liver tissue.Conclusion Human placenta extracts is effective in treating fatty liver disease induced by high-fatty diet.This may be due to its effects in lowering the levels of the cytokines such as Leptin、TNF-αand IL-6.