CAJ | 학술논문

目的:建立了脑立清丸等6种中成药中黄曲霉毒素G_2、G_1、B_2、B_1的HPLC测定方法.方法:样品经70%甲醇提取、免疫亲和柱净化后,用高效液相色谱-柱后衍生-荧光检测器进行分析测定.结果:黄曲霉毒素G_2、B_2在1.5～60 pg范围内线性关系良好,黄曲霉毒素G_1、B_1,在5～200 pg范围内线性关系良好,r＞0.999 9.回收率在60%～120%之间.结论:该方法快速简便,准确,可作为脑立清丸、人参养荣丸、人参健脾丸、三七片、金水宝胶囊和百令胶囊中黄曲霉毒素的含量测定方法.
목적:건립료뇌립청환등6충중성약중황곡매독소G_2、G_1、B_2、B_1적HPLC측정방법.방법:양품경70%갑순제취、면역친화주정화후,용고효액상색보-주후연생-형광검측기진행분석측정.결과:황곡매독소G_2、B_2재1.5～60 pg범위내선성관계량호,황곡매독소G_1、B_1,재5～200 pg범위내선성관계량호,r＞0.999 9.회수솔재60%～120%지간.결론:해방법쾌속간편,준학,가작위뇌립청환、인삼양영환、인삼건비환、삼칠편、금수보효낭화백령효낭중황곡매독소적함량측정방법.
AIM:To bring forward a method of determining aflatoxin G_2、G_1、B_2、B_1 in six kinds of traditional Chinese drugs by HPLC.METHODS:After being extracted by 70% methanol,purified by immunoaffinity column,aflatoxins were analysed by HPLC with fluorescence detection.RESULTS:Aflatoxin G_2、B_2 showed a good linear relationship at a range of 1.5-60pg,and Aflatoxin G_1、B_1 at a range of 5-200 pg,r＞0.999 9.The recovery was between 60%-120%.CONCLUSION:The method is simple,accurate and can be used to determine aflatoxin G_2、G_1、B_2、B_1 in Naoliqing Pill,Renshen Yangrong Pill,Rensen Jiapi Pill,Sanqi Tablet,Jinshuibao Capsule and Bailine Capsule.