农业科学与技术(英文版)
農業科學與技術(英文版)
농업과학여기술(영문판)
AGRICULTURAL SCIENCE & TECHNOLOGY
2010年
6期
112-114,182
,共4页
李拥军%李太坤%张丽%李文婷%张浩%施健飞
李擁軍%李太坤%張麗%李文婷%張浩%施健飛
리옹군%리태곤%장려%리문정%장호%시건비
海门山羊%MSTN基因%GH基因%多态性%生长性状
海門山羊%MSTN基因%GH基因%多態性%生長性狀
해문산양%MSTN기인%GH기인%다태성%생장성상
Haimen goats%MSTN%GH%Polymorphisms%Growth character
[目的]研究海门山羊群体3个基因位点遗传多态性与生长性状的关系,为提高海门山羊的生长性能以及培育快长型海门山羊新品系提供参考.[方法]采用PCR-RFLP和PCR-SSCP技术对不海门山羊群体3个基因位点进行分析,并进行相关统计分析.[结果]结果表明,在海门山羊群体中,海门山羊MSTN基因内含子Ⅱ、GH基因外显子Ⅰ和GH基因外显子Ⅱ均检测到2种基因型.这些位点不同的基因型对海门山羊的相关生长性状有着不同的影响.[结论] 该研究结果对通过标记辅助选择提高海门山羊体重等生长性状具有重要意义.
[目的]研究海門山羊群體3箇基因位點遺傳多態性與生長性狀的關繫,為提高海門山羊的生長性能以及培育快長型海門山羊新品繫提供參攷.[方法]採用PCR-RFLP和PCR-SSCP技術對不海門山羊群體3箇基因位點進行分析,併進行相關統計分析.[結果]結果錶明,在海門山羊群體中,海門山羊MSTN基因內含子Ⅱ、GH基因外顯子Ⅰ和GH基因外顯子Ⅱ均檢測到2種基因型.這些位點不同的基因型對海門山羊的相關生長性狀有著不同的影響.[結論] 該研究結果對通過標記輔助選擇提高海門山羊體重等生長性狀具有重要意義.
[목적]연구해문산양군체3개기인위점유전다태성여생장성상적관계,위제고해문산양적생장성능이급배육쾌장형해문산양신품계제공삼고.[방법]채용PCR-RFLP화PCR-SSCP기술대불해문산양군체3개기인위점진행분석,병진행상관통계분석.[결과]결과표명,재해문산양군체중,해문산양MSTN기인내함자Ⅱ、GH기인외현자Ⅰ화GH기인외현자Ⅱ균검측도2충기인형.저사위점불동적기인형대해문산양적상관생장성상유착불동적영향.[결론] 해연구결과대통과표기보조선택제고해문산양체중등생장성상구유중요의의.
[Objective] The paper aims to study the relationship between genetic polymorphisms of three gene loci and growth character of Haimen Goats to provide reference to promote Haimen Goat growth character and cultivate Haimen Goat new strain of fast-growth. [Method] We processed analysis and related statistical analysis on three gene loci of Haimen Goats with PCR-RFLP and PCR-SSCP. [Result] The results suggested that there were two genotypes in MSTN gene IntronⅡ, GH gene exonⅠ and GH gene exonⅡ in Haimen Goats. Different genotypes of these gene loci had different effects on relative growth character of Haimen Goats. [Conclusion] This study had a significant meaning about promoting Haimen Goat avoirdupois by marker assistant option.