国际中医中药杂志
國際中醫中藥雜誌
국제중의중약잡지
INTERNATIONAL JOURNAL OF TRIDITIONAL CHINESE MEDICINE
2009年
5期
389-390
,共2页
活血化瘀%缺血再灌注%中药预处理%心肌缺血预处理
活血化瘀%缺血再灌註%中藥預處理%心肌缺血預處理
활혈화어%결혈재관주%중약예처리%심기결혈예처리
Activating blood and dissolving stasis%Ischemia reperfusion%TCM preconditioning%Myocardial iscbemic preconditioning
目的 观察活血化瘀中药预处理和心肌缺血预处理对缺血一再灌注24 h大鼠血清NO、心肌HSP70影响.方法 选用雄性Wistar大鼠,随机分为4组,其中3组正常喂食、1组中药悬液灌胃,10 d后选各组健康大鼠行冠脉结扎,建立大鼠心肌缺血模型.假手术组:完成全部手术操作,只穿线不结扎冠脉.心肌缺血-再灌注组(IR组):模型建立后,心肌缺血45 min后再灌注24 h.活血化瘀中药预处理缺血-再灌注组(中药组):中药悬液0.32 g/100 g,连续10 d.模型建立后,心肌缺血45 min后再灌注24 h.预适应处理缺血-再灌注组(IP组):模型建立后,心肌缺血5 min,再灌注5 min,重复3次,之后心肌缺血45 min,再灌注24 h.采用荧光分光光度法查NO、免疫组化查HSP70.结果 IP组、中药组的NO含量、HSP70表达较IR组显著(P<0.05).结论 预适应心肌缺血再灌注、活血化瘀中药对NO含量、HSP70表达增加具有同效性.
目的 觀察活血化瘀中藥預處理和心肌缺血預處理對缺血一再灌註24 h大鼠血清NO、心肌HSP70影響.方法 選用雄性Wistar大鼠,隨機分為4組,其中3組正常餵食、1組中藥懸液灌胃,10 d後選各組健康大鼠行冠脈結扎,建立大鼠心肌缺血模型.假手術組:完成全部手術操作,隻穿線不結扎冠脈.心肌缺血-再灌註組(IR組):模型建立後,心肌缺血45 min後再灌註24 h.活血化瘀中藥預處理缺血-再灌註組(中藥組):中藥懸液0.32 g/100 g,連續10 d.模型建立後,心肌缺血45 min後再灌註24 h.預適應處理缺血-再灌註組(IP組):模型建立後,心肌缺血5 min,再灌註5 min,重複3次,之後心肌缺血45 min,再灌註24 h.採用熒光分光光度法查NO、免疫組化查HSP70.結果 IP組、中藥組的NO含量、HSP70錶達較IR組顯著(P<0.05).結論 預適應心肌缺血再灌註、活血化瘀中藥對NO含量、HSP70錶達增加具有同效性.
목적 관찰활혈화어중약예처리화심기결혈예처리대결혈일재관주24 h대서혈청NO、심기HSP70영향.방법 선용웅성Wistar대서,수궤분위4조,기중3조정상위식、1조중약현액관위,10 d후선각조건강대서행관맥결찰,건립대서심기결혈모형.가수술조:완성전부수술조작,지천선불결찰관맥.심기결혈-재관주조(IR조):모형건립후,심기결혈45 min후재관주24 h.활혈화어중약예처리결혈-재관주조(중약조):중약현액0.32 g/100 g,련속10 d.모형건립후,심기결혈45 min후재관주24 h.예괄응처리결혈-재관주조(IP조):모형건립후,심기결혈5 min,재관주5 min,중복3차,지후심기결혈45 min,재관주24 h.채용형광분광광도법사NO、면역조화사HSP70.결과 IP조、중약조적NO함량、HSP70표체교IR조현저(P<0.05).결론 예괄응심기결혈재관주、활혈화어중약대NO함량、HSP70표체증가구유동효성.
Objective To observe the effects of TCM preconditioning of activating blood and dissolving stasis and myocardial ischemic preconditioning on NO/serum and HSP70/myocardium of rats with ischemia reperfusion for 24 hours by ischemia reperfusion. Methods Male Wistar rats were randomly divided into 4 groups, three groups of which were fed normally, one of which was administered with suspension of Chinese medicine. 10 days later normal rats in each group were operated by coronary artery ligation to establish myocardial ischemia model. The rats of sham-operated group were performed in the same way as myocardial ischemia rats except the coronary artery was not ligated. The rats of IR group were reperfused for 24 hours after 45 min of myocardial ischemia. The rats of TCM group were treated with suspension of Chinese medicine administration of 0.32g/100g for 10 days before the same ischemia and reperfusion as IR group. And the rats of IP group were also performed the same ischemia and reperfusion as IR group after 5 min of ischemia and 5 min of reperfusion for three times. The NO/serum of rats was observed by fluorospectrophotometry and the HSP70/myocardium by immunohistochemistry. Results The results showed that the contents of NO/serum and expression of HSP70/myocardium in the IP group and TCM group of activating blood and dissolving stasis were expressed more significantly than the IP group (P<0.05) . Conclusion The group of myocardial ischemic preconditioning for ischemia reperfusion and the TCM group of activating blood and dissolving stasis have the similar effects on the contents of NO and expression of HSP70 increasing.
