CAJ | 학술논문

目的观察不同剂量粒-巨噬细胞集落刺激因子(GM-CSF)对小鼠树突状细胞(DC)形态、纯度、成熟程度的影响,为不同用途的DC疫苗制备选择合适的GM-CSF质量浓度提供理论依据.方法用低剂量(5 ng/ml)、常规剂量(20 ng/ml)、高剂量(50 ng/ml)GM-CSF联合重组小鼠白细胞介素-4(mIL-4)诱导小鼠骨髓细胞体外分化为DC,流式细胞术测定CD_(11c)、CD_(80)和CD_(86)表达率.结果低剂量GM-CSF组诱导的DC不具有典型的DC形态,低表达CD_(11c)、CD_(80)和CD_(86).常规剂量GM-CSF组诱导的DC高表达CD_(11c)、CD_(80)和CD_(86),具有更强的刺激同种异体淋巴细胞增殖能力.高剂量GM-CSF组CD_(11c)、CD_(80)和CD_(86)表达率最高,并具有最强的刺激同种异体淋巴细胞增殖的能力.结论 GM-CSF剂量影响诱导分化的DC成熟程度.低剂量的GM-CSF诱导的DC为不成熟的DC,常规剂量和高剂量GM-CSF诱导分化的DC为细胞表型和功能成熟的DC.高剂最GM-CSF诱导的DC细胞表型和功能最成熟.
목적 관찰불동제량립-거서세포집락자격인자(GM-CSF)대소서수돌상세포(DC)형태、순도、성숙정도적영향,위불동용도적DC역묘제비선택합괄적GM-CSF질량농도제공이론의거.방법 용저제량(5 ng/ml)、상규제량(20 ng/ml)、고제량(50 ng/ml)GM-CSF연합중조소서백세포개소-4(mIL-4)유도소서골수세포체외분화위DC,류식세포술측정CD_(11c)、CD_(80)화CD_(86)표체솔.결과 저제량GM-CSF조유도적DC불구유전형적DC형태,저표체CD_(11c)、CD_(80)화CD_(86).상규제량GM-CSF조유도적DC고표체CD_(11c)、CD_(80)화CD_(86),구유경강적자격동충이체림파세포증식능력.고제량GM-CSF조CD_(11c)、CD_(80)화CD_(86)표체솔최고,병구유최강적자격동충이체림파세포증식적능력.결론 GM-CSF제량영향유도분화적DC성숙정도.저제량적GM-CSF유도적DC위불성숙적DC,상규제량화고제량GM-CSF유도분화적DC위세포표형화공능성숙적DC.고제최GM-CSF유도적DC세포표형화공능최성숙.
Objective To observe the effects of different dosages of granulocyte-macrophage colony-stimulating factor (GM-CSF) on generating the routine bone marrow dendritic cells, and supply suitable dosage of GM-CSF on preparation of dendritic cell vaccines used for different purpose. Methods Using low (5 ng/ml) and conventional (20 ng/ml) and high dosage( 50 ng/ml ) of GM-CSF combined interleukin-4 ( IL-4 ) to induce murine bone marrow dendritic cells were performed, The phenotypes (CD_(11c), CD_(80), CD_(86)) and functional properties of the DC were compared by FACS analysis and MLR. Results The DC induced by low dosage of GM-CSF were immature DC, expressing low CD_(11c), CD_(80), and CD_(86). DC induced by conventional dosage were functional mature, expressing higher CD_(11c), CD_(80), CD_(86), which could induce allogeneic T lymphocyte responses. DC induced by the high dosage GM-CSF were the most phetotypicaUy and functional mature cells, expressing the highest CD_(11c), CD_(80) CD_(86), which could induce the strongest allogeneic T lymphocyte responses. Conclusion The dosages of GM-CSF affect the maturation stage of dendritic cells. Low dosage of GM-CSF generated immature dendritic cells, but conventional dosage and high dosage generated mature dendritic cells. DC generated through high dosage of GM-CSF were the most mature in phenotype and function.