肿瘤研究与临床
腫瘤研究與臨床
종류연구여림상
CANCER RESEARCH AND CLINIC
2012年
5期
291-294
,共4页
崔莹莹%冯盼盼%张祥宇%秦迎松
崔瑩瑩%馮盼盼%張祥宇%秦迎鬆
최형형%풍반반%장상우%진영송
癌症疫苗%抗原,肿瘤%肿瘤细胞,培养的%冻融肿瘤细胞%细胞培养技术
癌癥疫苗%抗原,腫瘤%腫瘤細胞,培養的%凍融腫瘤細胞%細胞培養技術
암증역묘%항원,종류%종류세포,배양적%동융종류세포%세포배양기술
Cancer vaccines%Antigens,neoplasm%Tumor cells,cultured%Freeze-thaw tumor cell%Cell culture techniques
目的 比较冻融肿瘤细胞和肿瘤细胞培养上清对淋巴细胞活化能力影响的差异.方法 常规冻融法制备恶性黑色素瘤B16细胞全肿瘤细胞抗原,收集培养不同时间的肿瘤细胞培养上清；利用Transwell法检测冻融肿瘤细胞以及肿瘤细胞培养上清对淋巴细胞的趋化能力；用CCK-8法检测各组趋化淋巴细胞的肿瘤杀伤活性.结果 冻融肿瘤细胞及培养2h以上的肿瘤上清液对淋巴细胞均有趋化作用；培养4h以上的肿瘤上清液的趋化能力明显强于冻融瘤细胞.各组趋化的淋巴细胞均具有肿瘤杀伤活性；培养4h以上的肿瘤上清液组淋巴细胞的杀伤能力明显强于冻融肿瘤细胞组.在一定范围内,培养上清对淋巴细胞的趋化和活化能力随时间延长而增强.结论 培养一定时间的肿瘤上清液对淋巴细胞的趋化和活化能力均强于冻融肿瘤细胞,用培养上清液代替冻融肿瘤细胞抗原作为抗原活化淋巴细胞可获得更好的免疫效果.
目的 比較凍融腫瘤細胞和腫瘤細胞培養上清對淋巴細胞活化能力影響的差異.方法 常規凍融法製備噁性黑色素瘤B16細胞全腫瘤細胞抗原,收集培養不同時間的腫瘤細胞培養上清；利用Transwell法檢測凍融腫瘤細胞以及腫瘤細胞培養上清對淋巴細胞的趨化能力；用CCK-8法檢測各組趨化淋巴細胞的腫瘤殺傷活性.結果 凍融腫瘤細胞及培養2h以上的腫瘤上清液對淋巴細胞均有趨化作用；培養4h以上的腫瘤上清液的趨化能力明顯彊于凍融瘤細胞.各組趨化的淋巴細胞均具有腫瘤殺傷活性；培養4h以上的腫瘤上清液組淋巴細胞的殺傷能力明顯彊于凍融腫瘤細胞組.在一定範圍內,培養上清對淋巴細胞的趨化和活化能力隨時間延長而增彊.結論 培養一定時間的腫瘤上清液對淋巴細胞的趨化和活化能力均彊于凍融腫瘤細胞,用培養上清液代替凍融腫瘤細胞抗原作為抗原活化淋巴細胞可穫得更好的免疫效果.
목적 비교동융종류세포화종류세포배양상청대림파세포활화능력영향적차이.방법 상규동융법제비악성흑색소류B16세포전종류세포항원,수집배양불동시간적종류세포배양상청；이용Transwell법검측동융종류세포이급종류세포배양상청대림파세포적추화능력；용CCK-8법검측각조추화림파세포적종류살상활성.결과 동융종류세포급배양2h이상적종류상청액대림파세포균유추화작용；배양4h이상적종류상청액적추화능력명현강우동융류세포.각조추화적림파세포균구유종류살상활성；배양4h이상적종류상청액조림파세포적살상능력명현강우동융종류세포조.재일정범위내,배양상청대림파세포적추화화활화능력수시간연장이증강.결론 배양일정시간적종류상청액대림파세포적추화화활화능력균강우동융종류세포,용배양상청액대체동융종류세포항원작위항원활화림파세포가획득경호적면역효과.
Objective To compare the effect of freeze-thaw tumor cells and the supernatant from tumor cell culture on the activation of lymphocytes. Methods Malignant melanoma B16 cells were prepared as tumor cell vaccine and the supernatant from tumor cell culture was collected at different time point.Transwell method was used to determine the chemotaxis of lymphocyte attracted by freeze-thaw tumor cells and the supernatant from tumnor cell culture. The cytotoxic activity of lymphocyte was detected by CCK-8 method. Results Freeze-thaw tumor cells and the supernatant from more than 2 h of tumor cell culture were found to show chemotaxis of lymphocyte. The chemotaxis of tumor cell culture more than 4 h was stronger than freeze-thaw tumor cells. Each group of chemotactic lymphocytes demonstrated to have the activity of killing tumor cells. The ability of killing tumor cells induced by the tumor cell culture more than 4 h was stronger than that induced by freeze-thaw tumor cells.In a certain range,the ability of lymphocyte chemotaxis and activation were enhanced over time. Conclusion The chemotaxis and cytotoxic activation of lymphocyte induced by the supernatant from tumor cell culture for a certain time are stronger than those by freeze-thaw tumor cells. The supernatant from tumor cell culture can be used as tumor antigen to get better immune activation instead of the freeze-thaw tumor cells.
