中国实验血液学杂志
中國實驗血液學雜誌
중국실험혈액학잡지
JOURNAL OF EXPERIMENTAL HEMATOLOGY
2004年
4期
498-502
,共5页
细胞因子%T淋巴细胞%同种异体反应%急性移植物抗宿主病
細胞因子%T淋巴細胞%同種異體反應%急性移植物抗宿主病
세포인자%T림파세포%동충이체반응%급성이식물항숙주병
cytokine%T lymphocyte%allogeneic reaction%acute graft versus host disease
本研究目的是测定同种异基因外周血单个核细胞(PBMNCs)刺激后分泌不同细胞因子的T淋巴细胞水平并对其临床意义进行研究,以建立一种监测同种异体反应性T淋巴细胞的新方法.首先,利用新颖的细胞因子分泌检测方法(CKSA)从单细胞水平定量测定了人混合淋巴细胞反应后分泌IFN-γ,IL-4和IL-l0的T淋巴细胞水平;然后根据上述实验结果,分析2例接受异基因骨髓移植后发生急性移植物抗宿主病(aGVHD)的患者外周血中分泌IFN-γ的T细胞水平.结果表明:经同种异体PBMNCs刺激后分泌IFN-γ的T淋巴细胞水平显著升高[(1.12±0.13)%],而分泌IL-4和IL-10的T淋巴细胞则无升高,分别为(0.12±0.03)%和(0.10±0.03)%.患者外周血分泌IFN-γ的T淋巴细胞水平和aGVHD的发生及严重程度有一定的相关性.结论:利用CKSA从单细胞水平定量测定同种异体PBMNCs刺激后分泌IFN-γ的T淋巴细胞水平的技术具有临床应用价值,可以应用于对aGVHD的识别.
本研究目的是測定同種異基因外週血單箇覈細胞(PBMNCs)刺激後分泌不同細胞因子的T淋巴細胞水平併對其臨床意義進行研究,以建立一種鑑測同種異體反應性T淋巴細胞的新方法.首先,利用新穎的細胞因子分泌檢測方法(CKSA)從單細胞水平定量測定瞭人混閤淋巴細胞反應後分泌IFN-γ,IL-4和IL-l0的T淋巴細胞水平;然後根據上述實驗結果,分析2例接受異基因骨髓移植後髮生急性移植物抗宿主病(aGVHD)的患者外週血中分泌IFN-γ的T細胞水平.結果錶明:經同種異體PBMNCs刺激後分泌IFN-γ的T淋巴細胞水平顯著升高[(1.12±0.13)%],而分泌IL-4和IL-10的T淋巴細胞則無升高,分彆為(0.12±0.03)%和(0.10±0.03)%.患者外週血分泌IFN-γ的T淋巴細胞水平和aGVHD的髮生及嚴重程度有一定的相關性.結論:利用CKSA從單細胞水平定量測定同種異體PBMNCs刺激後分泌IFN-γ的T淋巴細胞水平的技術具有臨床應用價值,可以應用于對aGVHD的識彆.
본연구목적시측정동충이기인외주혈단개핵세포(PBMNCs)자격후분비불동세포인자적T림파세포수평병대기림상의의진행연구,이건립일충감측동충이체반응성T림파세포적신방법.수선,이용신영적세포인자분비검측방법(CKSA)종단세포수평정량측정료인혼합림파세포반응후분비IFN-γ,IL-4화IL-l0적T림파세포수평;연후근거상술실험결과,분석2례접수이기인골수이식후발생급성이식물항숙주병(aGVHD)적환자외주혈중분비IFN-γ적T세포수평.결과표명:경동충이체PBMNCs자격후분비IFN-γ적T림파세포수평현저승고[(1.12±0.13)%],이분비IL-4화IL-10적T림파세포칙무승고,분별위(0.12±0.03)%화(0.10±0.03)%.환자외주혈분비IFN-γ적T림파세포수평화aGVHD적발생급엄중정도유일정적상관성.결론:이용CKSA종단세포수평정량측정동충이체PBMNCs자격후분비IFN-γ적T림파세포수평적기술구유림상응용개치,가이응용우대aGVHD적식별.
In order to explore a new way to study allogeneic reactive T lymphocytes, detection of cytokine-secreting T lymphocytes after allogeneic peripheral blood mononuclear cells (PBMNCs) stimulation and investigation of its clinical significance were performed. A novel cytokine secretion assay (CKSA) was first applied to detect T lymphocytes secreting cytokine including IFN-γ, IL-4 and IL-10 at single cell level in human mixed lymphocytes reaction. IFN-γ-secreting T cells from PBMNCs were then evaluated in 2 patients with acute graft versus host disease (aGVHD) after allogeneic bone marrow transplantation. The results showed that compared with IL-4 and IL-10 (which were 0.12 ± 0.03% and 0.10 ±0.03% respectively), a sizable proportion of IFN-γ-secreting T lymphocytes could be detected (1.12 ± 0.13)% after allogeneic PBMNCs stimulation. Preliminary results indicated that frequency of IFN-γ-secreting T lymphocytes correlated with the onset and severity of clinical aGVHD. In conclusion, it is feasible to detect IFN-γ secreting T lymphocytes after allogeneic PBMNCs stimulation and to apply the CKSA technique for clinical identification of aGVHD.
