背景:甘蔗蜡作为制糖工业的副产品,在非医药方面已有广泛的用途.有实验证实,甘蔗蜡在降低血胆固醇方面具有良好的作用,但其效应及适用于医药应用的甘蔗蜡的分离手段有待进一步研究.目的:从国产甘蔗蜡中分离适合医药用途的高级烷酸混合物、高级烷醇混合物,并观察其对高脂血症模型大鼠的降胆固醇作用.设计:随机对照动物实验.单位:重庆康尔威药业股份有限公司药物研究所.材料:实验于2005-04/2006-01在重庆康尔威药业股份有限公司药物研究所完成.选用65只成年Wistar雌性大白鼠,体质量180～220 g,鼠龄3,～6个月,SPF级,由重庆中药研究院实验动物中心提供.粗甘蔗蜡购自北京嘉德鸿盛生物化学科技有限公司.26C醇、28C醇、30C醇、32C醇和28C酸、30C酸、32C酸、34C酸为Sigma产品(气相色谱标准品).其余试剂为国产分析纯级.方法:①将粗甘蔗蜡通过乙醇等有机溶剂反复提取后成为精制甘蔗蜡,进一步通过皂化、加钙等,将高级烷酸混合物与高级烷醇混合物分离.用气相色谱分析其主要成分,高级烷醇混合物主要分析C26、C28、C30和C32,高级烷酸混合物主要分析C26、C28、C30和C32.②按照参考文献叙述的方法,将大白鼠适应性饲养3d后,随机摸球法分为空白组(n=10)和实验组(n=55),断尾采血,自动生化分析仪测定所有大鼠三酰甘油、总胆固醇与高密度脂蛋白胆固醇.空白组给予普通颗粒饲料,实验组给予高脂饲料(猪油质量分数0.1、蛋黄粉质量分数0.1、胆固醇质量分数0.01、猪胆盐质量分数0.002、普通饲料质量分数0.788)[3],自由摄食与饮水.7 d后尾尖再次采血测定三酰甘油、总胆固醇与高密度脂蛋白胆固醇.按血清三酰甘油的高低随机将实验组大白鼠分为5个亚组:阴性对照组、高级烷醇低剂量组、高级烷醇高剂量组、高级烷酸低剂量组、高级烷酸高剂量组,每组11只.高级烷醇高、低剂量组实验鼠灌服5,50 mg/(kg·d)高级烷醇,高级烷酸高、低剂量组实验鼠分别给予分别给予灌服20,200 mg/kg·d高级烷酸,阴性对照组及空白组分别给予灌服等体积的0.3%羧甲基纤维素钠及蒸馏水,均为1次/d.连续给药30 d,最后1次给药16 h后,于麻醉状态下心脏采血,取血清测定三酰甘油、总胆固醇与高密度脂蛋白胆固醇.主要观察指标:①分离的高级烷醇混合物、高级烷醇混合物中主要成分含量的百分比.②实验鼠用药前后血胆固醇、高密度脂蛋白和三酰甘油水平.结果:纳入实验鼠65只均进入结果分析.①通过气相色谱分析分析表明,在高级烷醇混合物中以28烷醇(C28醇)含量最多(占73.6%),其主要几种高级烷醇分别为C26醇(5.3%)、C30醇(6.2%)、C32醇(5.1%)4种高级烷醇总量为90.2%.在高级烷酸混合物中,以28碳酸(C28酸)含量最多(占46.6%),其余几种主要高级烷酸分别为C30酸(16.7%)、C32酸(6.8%)、C34酸(9.3%).4种高级烷酸总量为79.3%.②高级烷醇高、低剂量组及高级烷酸高、低剂量组实验鼠血清总胆固醇水平分别为(1.46±0.27),(1.66±0.33),(1.44±0.25),(2.16±0.52)mmol/L,低于阴性对照组[(2.52±0.83)mmol/L,P＜0.01].高血高密度脂蛋白胆固醇水平分别为(0.73±0.09),(0.71±0.07),(0.79±0.10),(0,70±0.08)mmol/L,高于阴性对照组[(0.58±0.13)mmol/L,P＜0.05～0.01].结论:从国产甘蔗蜡中分离的高级烷醇混合物、高级烷酸混合物均有明显的降低血总胆固醇作用,对降低血三酰甘油效果不明显.
揹景:甘蔗蠟作為製糖工業的副產品,在非醫藥方麵已有廣汎的用途.有實驗證實,甘蔗蠟在降低血膽固醇方麵具有良好的作用,但其效應及適用于醫藥應用的甘蔗蠟的分離手段有待進一步研究.目的:從國產甘蔗蠟中分離適閤醫藥用途的高級烷痠混閤物、高級烷醇混閤物,併觀察其對高脂血癥模型大鼠的降膽固醇作用.設計:隨機對照動物實驗.單位:重慶康爾威藥業股份有限公司藥物研究所.材料:實驗于2005-04/2006-01在重慶康爾威藥業股份有限公司藥物研究所完成.選用65隻成年Wistar雌性大白鼠,體質量180～220 g,鼠齡3,～6箇月,SPF級,由重慶中藥研究院實驗動物中心提供.粗甘蔗蠟購自北京嘉德鴻盛生物化學科技有限公司.26C醇、28C醇、30C醇、32C醇和28C痠、30C痠、32C痠、34C痠為Sigma產品(氣相色譜標準品).其餘試劑為國產分析純級.方法:①將粗甘蔗蠟通過乙醇等有機溶劑反複提取後成為精製甘蔗蠟,進一步通過皂化、加鈣等,將高級烷痠混閤物與高級烷醇混閤物分離.用氣相色譜分析其主要成分,高級烷醇混閤物主要分析C26、C28、C30和C32,高級烷痠混閤物主要分析C26、C28、C30和C32.②按照參攷文獻敘述的方法,將大白鼠適應性飼養3d後,隨機摸毬法分為空白組(n=10)和實驗組(n=55),斷尾採血,自動生化分析儀測定所有大鼠三酰甘油、總膽固醇與高密度脂蛋白膽固醇.空白組給予普通顆粒飼料,實驗組給予高脂飼料(豬油質量分數0.1、蛋黃粉質量分數0.1、膽固醇質量分數0.01、豬膽鹽質量分數0.002、普通飼料質量分數0.788)[3],自由攝食與飲水.7 d後尾尖再次採血測定三酰甘油、總膽固醇與高密度脂蛋白膽固醇.按血清三酰甘油的高低隨機將實驗組大白鼠分為5箇亞組:陰性對照組、高級烷醇低劑量組、高級烷醇高劑量組、高級烷痠低劑量組、高級烷痠高劑量組,每組11隻.高級烷醇高、低劑量組實驗鼠灌服5,50 mg/(kg·d)高級烷醇,高級烷痠高、低劑量組實驗鼠分彆給予分彆給予灌服20,200 mg/kg·d高級烷痠,陰性對照組及空白組分彆給予灌服等體積的0.3%羧甲基纖維素鈉及蒸餾水,均為1次/d.連續給藥30 d,最後1次給藥16 h後,于痳醉狀態下心髒採血,取血清測定三酰甘油、總膽固醇與高密度脂蛋白膽固醇.主要觀察指標:①分離的高級烷醇混閤物、高級烷醇混閤物中主要成分含量的百分比.②實驗鼠用藥前後血膽固醇、高密度脂蛋白和三酰甘油水平.結果:納入實驗鼠65隻均進入結果分析.①通過氣相色譜分析分析錶明,在高級烷醇混閤物中以28烷醇(C28醇)含量最多(佔73.6%),其主要幾種高級烷醇分彆為C26醇(5.3%)、C30醇(6.2%)、C32醇(5.1%)4種高級烷醇總量為90.2%.在高級烷痠混閤物中,以28碳痠(C28痠)含量最多(佔46.6%),其餘幾種主要高級烷痠分彆為C30痠(16.7%)、C32痠(6.8%)、C34痠(9.3%).4種高級烷痠總量為79.3%.②高級烷醇高、低劑量組及高級烷痠高、低劑量組實驗鼠血清總膽固醇水平分彆為(1.46±0.27),(1.66±0.33),(1.44±0.25),(2.16±0.52)mmol/L,低于陰性對照組[(2.52±0.83)mmol/L,P＜0.01].高血高密度脂蛋白膽固醇水平分彆為(0.73±0.09),(0.71±0.07),(0.79±0.10),(0,70±0.08)mmol/L,高于陰性對照組[(0.58±0.13)mmol/L,P＜0.05～0.01].結論:從國產甘蔗蠟中分離的高級烷醇混閤物、高級烷痠混閤物均有明顯的降低血總膽固醇作用,對降低血三酰甘油效果不明顯.
배경:감자사작위제당공업적부산품,재비의약방면이유엄범적용도.유실험증실,감자사재강저혈담고순방면구유량호적작용,단기효응급괄용우의약응용적감자사적분리수단유대진일보연구.목적:종국산감자사중분리괄합의약용도적고급완산혼합물、고급완순혼합물,병관찰기대고지혈증모형대서적강담고순작용.설계:수궤대조동물실험.단위:중경강이위약업고빈유한공사약물연구소.재료:실험우2005-04/2006-01재중경강이위약업고빈유한공사약물연구소완성.선용65지성년Wistar자성대백서,체질량180～220 g,서령3,～6개월,SPF급,유중경중약연구원실험동물중심제공.조감자사구자북경가덕홍성생물화학과기유한공사.26C순、28C순、30C순、32C순화28C산、30C산、32C산、34C산위Sigma산품(기상색보표준품).기여시제위국산분석순급.방법:①장조감자사통과을순등유궤용제반복제취후성위정제감자사,진일보통과조화、가개등,장고급완산혼합물여고급완순혼합물분리.용기상색보분석기주요성분,고급완순혼합물주요분석C26、C28、C30화C32,고급완산혼합물주요분석C26、C28、C30화C32.②안조삼고문헌서술적방법,장대백서괄응성사양3d후,수궤모구법분위공백조(n=10)화실험조(n=55),단미채혈,자동생화분석의측정소유대서삼선감유、총담고순여고밀도지단백담고순.공백조급여보통과립사료,실험조급여고지사료(저유질량분수0.1、단황분질량분수0.1、담고순질량분수0.01、저담염질량분수0.002、보통사료질량분수0.788)[3],자유섭식여음수.7 d후미첨재차채혈측정삼선감유、총담고순여고밀도지단백담고순.안혈청삼선감유적고저수궤장실험조대백서분위5개아조:음성대조조、고급완순저제량조、고급완순고제량조、고급완산저제량조、고급완산고제량조,매조11지.고급완순고、저제량조실험서관복5,50 mg/(kg·d)고급완순,고급완산고、저제량조실험서분별급여분별급여관복20,200 mg/kg·d고급완산,음성대조조급공백조분별급여관복등체적적0.3%최갑기섬유소납급증류수,균위1차/d.련속급약30 d,최후1차급약16 h후,우마취상태하심장채혈,취혈청측정삼선감유、총담고순여고밀도지단백담고순.주요관찰지표:①분리적고급완순혼합물、고급완순혼합물중주요성분함량적백분비.②실험서용약전후혈담고순、고밀도지단백화삼선감유수평.결과:납입실험서65지균진입결과분석.①통과기상색보분석분석표명,재고급완순혼합물중이28완순(C28순)함량최다(점73.6%),기주요궤충고급완순분별위C26순(5.3%)、C30순(6.2%)、C32순(5.1%)4충고급완순총량위90.2%.재고급완산혼합물중,이28탄산(C28산)함량최다(점46.6%),기여궤충주요고급완산분별위C30산(16.7%)、C32산(6.8%)、C34산(9.3%).4충고급완산총량위79.3%.②고급완순고、저제량조급고급완산고、저제량조실험서혈청총담고순수평분별위(1.46±0.27),(1.66±0.33),(1.44±0.25),(2.16±0.52)mmol/L,저우음성대조조[(2.52±0.83)mmol/L,P＜0.01].고혈고밀도지단백담고순수평분별위(0.73±0.09),(0.71±0.07),(0.79±0.10),(0,70±0.08)mmol/L,고우음성대조조[(0.58±0.13)mmol/L,P＜0.05～0.01].결론:종국산감자사중분리적고급완순혼합물、고급완산혼합물균유명현적강저혈총담고순작용,대강저혈삼선감유효과불명현.
BACKGROUND:As a by-product in sugar industry, sugar cane wax has been widely used in non-medical field. Some researches indicate that sugar cane wax plays a great role in reducing blood cholesterol; however, the therapeutical effect and clinical application should be studied further.OBJECTIVE: To separate the high-class eicosanoic acid and the high-class alkanols, which are suitable for medical application, and further to observe the effect of them on reducing blood cholesterol of model rets with hyperlipemia.DESIGN: Randomized control animal study.SETTING: Pharmacological Institute, Chongqing Kangerwei Pharmaceutical Co., Ltd.MATERIALS.: The experiment was carried out in the Pharmacological Institute of Chongqing K.E.W Pharmaceutical Go.,Ltd. From April 2005 to January 2006. A total of 65 adult female Wistar rats, aged at 3-6 months, weighing 180-220 g, of SPF grade, were provided by Experimental Animal Center of Chongqing Institute of Traditional Chinese Medicine. Raw sugar cane wax was provided by Beijing Jiade Hongsheng Biochemical Technology Co., Ltd. High-class alkanols C26,C28,C30, C32 and high-class eicosanoic acid C28, C30, C32, C34 were provided by Sigma Company (standard materials of gas phase chromatography), and other reagents were national analytical pure.METHODS: ① Sugar cane wax was extracted from raw sugar cane wax with ethanol and other organic solution and separated from the mixture of high-class eicosanoic acid and the mixture of high-class alkanols with saponification and calcification. Main components were analyzed with gas phase chromatography. The main components of high-class alkanols were C26, C28, C30 and C32 and the main components of high-class eicosanoic acid were C28, C30, C32 and C34, ② Based on references, rats were fed in 3 days and randomly divided into blank group (n =10) and experimental group (n =55).And then, all rats were cut off their tails to collect blood and the triacylglyoerol (TG), total cholesterol (TC) and high-density lipoprotein cholesterol (HDL-C) were measured with automatic biochemistry analyzer. Rats were fed with common granule feeds in blank group or with high-lipid feeds (containing 0.1 mass fraction of oiliness, 0.1 mass fraction of yolk powder, 0.01 mass fraction of cholesterol, 0.002 mass fraction of pig's gall salt, 0.788 mass fraction of common feeds) in experimental group. All rats ate and drank freely. Seven days later, blood was collected again from tail tip to measure the contents of TG, TC and HDL-C. Based on level of serum TG, rats in the experimental group were randomly divided into 5subgroups (n =11): negative control group, low-dosage high-class alkanols group, high-dosage high-class alkanols group,Iowdosage high-class eicosanoic acid and high-dosage high-class eicosanoic acid group. Rats in low-dosage and high-dosage high-class alkanols groups were perfused with 5 and 50 mg/(kg·d) high-class alkanols; meanwhile, rats in low-dosage and high-dosage high-class eicosanoic acid groups were perfused with 20 and 200 mg/ (kg·d) high-class eicosanoic acid. Rats in negative control group and blank group were perfused with the same volume of 0.3% carboxymethylcellulose sodium and distilled water, respectively, once a day for successive 30 days. At 16 hours after the last administration, rats were anesthetized to collect blood from heart to measure contents of TG, TC and HDL-C in serum.MAIN OUTCOME MEASURES: ① Percentage of main component in separated mixtures of high-class eicosanoic acid high-class alkanols; ② levels of serum cholesterol, HDL and TG.RESULTS: A total of 65 experimental rats were involved in the final analysis. ① Gas phase chromatography suggested that the content of C28 high-class alkanols was the most (73.6%), and other three kinds of high-class alkanols were counted for 5.3% (C26), 6.2% (C30) and 5.1% (C32), respectively. The total quantity was 90.2%. In the mixture of high-class eicosanoic acid, content of C28 high-class eicosanoic acid was the most (46.6%) and the other three kinds of high-class eicosanoic acid were counted for 16.7% (C30), 6.8% (C32) and 9.3% (C34), respectively. The total quantity was 79.3%. ②Levels of serum TC were (1.46±0.27), (1.66±0.33), (1.44±0.25) and (2.16±0.52) mmol/L in high-dosage and Iow-dosage high-class alkanols groups and high-dosage and Iow-dosage high-class eicosanoic acid groups, respectively, which were lower than those in negative control group [(2.52±0.83) mmol/L, P＜0.01]. Levels of HDL-C were (0.73±0.09), (0.71±0.07), (0.79±0.10) and (0.70±0.08) mmol/L in the four treatment groups, respectively, which were higher than those in negative control group [(0.58±0.13) mmol/L, P＜0.05-0.01].CONCLUSION: The high-class alkanols and the high-class eicosanoic acids separated from sugar cane wax made in China significantly have the activity of reducing blood cholesterol; however, the effect on decreasing TG is not obvious.
